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2015 | 37 | 01 |
Tytuł artykułu

Differential proteomic analysis of dwarf characteristics in cucumber (Cucumis sativus Linn.) stems

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Dwarfism was one of the most important agronomic traits in cucumber breeding. The current study was conducted to identify dwarf-related proteins using twodimensional electrophoresis. Twenty-two differentially expressed protein spots were detected between dwarf and vine genotypes while 20 of them were successfully identified by MALDI-TOF/TOF MS. Out of 20 identified proteins in dwarf genotype, 14 were up-regulated and six were down-regulated. The classification of differential proteins showed that the identified proteins were functionally involved in photosynthesis, energy metabolism, cytoskeletal functions, transduction and signal regulation, detoxification and redox regulation. Five differentially regulated proteins were analyzed using the technique quantitative real-time PCR (qRT-PCR). The results showed that four proteins, including histone deacetylase, the EIN2- like protein, chlorophyll A/B binding protein, and ubiquitin-conjugating enzyme Spm2 (E2) were up-regulated and one F-box family protein was down-regulated in the dwarf genotype. Further western blot analysis revealed that only E2 protein was detected in the dwarf genotype, indicating its important role in the cucumber dwarf trait.
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Article: 1703 [13 p.], fig.,ref.
  • College of Horticulture, Northeast Agricultural University, Harbin, Heilongjiang, China
  • College of Horticulture, Northeast Agricultural University, Harbin, Heilongjiang, China
  • College of Resources and Environment, Northeast Agricultural University, Harbin, Heilongjiang, China
  • College of Life Science, Northeast Agricultural University, Harbin, Heilongjiang, China
  • College of Horticulture, Northeast Agricultural University, Harbin, Heilongjiang, China
  • College of Horticulture, Northeast Agricultural University, Harbin, Heilongjiang, China
  • Abbasi F, Onodera H, Toki S, Tanaka H, Komatsu S (2004) OsCDPK13, a calcium-dependent protein kinase gene from rice, is induced by cold and gibberellin in rice leaf sheath. Plant Mol Biol 55(4):541–552
  • Boudsocq M, Sheen J (2013) CDPKs in immune and stress signaling. Trends Plant Sci 18:30–40
  • Buda GJ, Barnes WJ, Fich EA, Park S, Yeats TH, Zhao L, Domozych DS, Rose JK (2013) An ATP binding cassette transporter is required for cuticular wax deposition and desiccation tolerance in the moss Physcomitrella patens. Plant Cell 25(10):4000–4013
  • Chae L, Sudat S, Dudoit S, Zhu T, Luan S (2009) Diverse transcriptional programs associated with environmental stress and hormones in the Arabidopsis receptor-like kinase gene family. Mol Plant 2(1):84–107
  • Chattopadhyay A, Subba P, Pandey A, Bhushan D, Kumar R, Datta A, Chakraborty S, Chakraborty N (2011) Analysis of the grasspea proteome and identification of stress-responsive proteins upon exposure to high salinity, low temperature, and abscisic acid treatment. Phytochemistry 72:1293–1307
  • Chen S, Yuan HM, Liu GF, Li HY, Jiang J (2012) A label-free differential quantitative proteomics analysis of a TaLEA-introduced transgenic Populus simonii 9 Populus nigra dwarf mutant. Mol Biol Rep 39(7):7657–7664
  • Degenkolb T, Gams W, Bru¨ckner H (2008) Natural cyclopeptaibiotics and related cyclic tetrapeptides: structural diversity and future prospects. Chem Biodivers 5(5):693–706
  • Ducket CM, Lloyd C (1994) Gibberellic acid-induced microtubule reorientation in dwarf peas is accompanied by rapid modification of an a-tubulin isotype. Plant J 5:363–372
  • Fleet CM, Sun TP (2005) A DELLAcate balance: the role of gibberellin in plant morphogenesis. Curr Opin Plant Biol 8:77–85
  • Franco OL, Pelegrini PB, Gomes CP, Souza A, Costa FT, Domont G, Quirino BF, Eira MT, Mehta A (2009) Proteomic evaluation of coffee zygotic embryos in two different stages of seed development. Plant Physiol Biochem 47(11–12):1046–1050
  • Furlan G, Klinkenberg J, Trujillo M (2012) Regulation of plant immune receptors by ubiquitination. Front Plant Sci 3:238
  • Fu X, Richards DE, Fleck B, Xie D, Burton N, Harberd NP (2004) The Arabidopsis mutant sleepy1gar2-1 protein promotes plant growth by increasing the affinity of the SCFSLY1 E3 ubiquitin ligase for DELLA protein substrates. Plant Cell 16:1406–1418
  • Gao Z, Qian Q, Liu X, Yan M, Feng Q, Dong G, Liu J, Han B (2009) Dwarf 88, a novel putative esterase gene affecting architecture of rice plant. Plant Mol Biol 71(3):265–276
  • Geisler M, Murphy AS (2006) The ABC of auxin transport: the role of p-glycoproteins in plant development. FEBS Lett 580(4):1094–1102
  • Ghosh S, Pal A (2013) Proteomic analysis of cotyledonary explants during shoot organogenesis in Vigna radiate. Plant Cell Tiss Organ Cult 115(1):55–68
  • Gomi K, Sasaki A, Itoh H et al (2004) GID2, an F-box subunit of the SCF E3 complex, specifically interacts with phosphorylated SLR1 protein and regulates the gibberellin-dependent degradation of SLR1 in rice. Plant J 37:626–634
  • Hall AE, Bleecker AB (2003) Analysis of combinatorial loss-of function mutants in the Arabidopsis ethylene receptors reveals that the ers1 etr1 double mutant has severe developmental defects that are EIN2 dependent. Plant Cell 15(9):2032–2041
  • Harrak H, Azelmat S, Baker EN, Tabaeizadeh Z (2001) Isolation and characterization of a gene encoding a drought-induced cysteine protease in tomato (Lycopersicon esculentum).Genome44:368–374
  • He Y, Tang RH, Hao Y, Stevens RD, Cook CW, Ahn SM, Jing L, Yang Z, Chen L, Guo F, Fiorani F, Jackson RB, Crawford NM, Pei ZM (2004) Nitric oxide represses the Arabidopsis floral transition. Science 305(5692):1968–1971
  • Hedden P (2003) The genes of the green revolution. Trends Genet 19:5–9
  • Hou DY, Xu H, Du GY, Lin JT, Duan M, Guo AG (2009) Proteome analysis of chloroplast proteins in stage albinism line of winter wheat (triticum aestivum) FA85. BMB Rep 42(7):450–455
  • Huang J, Tang D, Shen Y, Qin BX, Hong LL, You AQ, Li M, Wang X, Yu HX, Gu MH, Cheng ZK (2010) Activation of gibberellin 2-oxidase 6 decreases active gibberellin levels and creates a dominant semi-dwarf phenotype in rice (Oryza sativa L.). J Genet Genomics 37(1):23–36
  • Itoh H, Matsuoka M, Steber CM (2003) A role for the ubiquitin–26Sproteasome pathway in gibberellin signaling. Trends Plant Sci 8:492–497
  • Kiselev KV, Dubrovina AS, Shumakova OA, Karetin YA, Manyakhin AY (2013) Structure and expression profiling of a novel calciumdependent protein kinase gene, CDPK3a, in leaves, stems, grapes, and cell cultures of wild-growing grapevine Vitis amurensis Rupr. Plant Cell Rep 32(3):431–442
  • Kiyosaki T, Matsumoto I, Asakura T, Funaki J, Kuroda M, Misaka T, Arai S, Abe K (2007) Gliadain, a gibberellin-inducible cysteine proteinase occurring in germinating seeds of wheat, Triticum aestivum L., specifically digests gliadin and is regulated by intrinsic cystatins. FEBS J 274(8):1908–1917
  • Komatsu S, Konishi H (2005) Proteome analysis of rice root proteins regulated by gibberellin. Genomics Proteomics Bioinform 3(3):132–142
  • Konishi H, Yamane H, Maeshima M, Komatsu S (2004) Characterization of fructose-bisphosphate aldolase regulated by gibberellin in roots of rice seedling. Plant Mol Biol 56(6):839–848
  • Konstantin VK, Anna VT, Yuri NZ (2010) Structure and expression profiling of a novel calcium-dependent protein kinase gene PgCDPK1a in roots, leaves, and cell cultures of Panax ginseng. Plant Cell Tiss Organ Cult 103:197–204
  • Lang JM, Eisinger WR, Green PB (1982) Effects of ethylene on the orientation of microtubules and cellulose microfibrils of pea epicotyls with polylamellate cell walls. Protoplasma 110:5–14
  • Lehman A, Black R, Ecker JR (1996) HOOKLESS1, an ethylene response gene, is required for differential cell elongation in the Arabidopsis hypocotyl. Cell 85(2):183–194
  • Li B, Xu W, Xu Y, Zhang Y, Wang T, Bai Y, Han C, Zhang A, Xu Z, Chong K (2010) Integrative study on proteomics, molecular physiology, and genetics reveals an accumulation of cyclophilinlike protein, TaCYP20-2, leading to an increase of Rht protein and dwarf in a novel GA-insensitive mutant (gaid) in Wheat. J Proteome Res 9(8):4242–4253
  • Li YH, Yang LM, Pathak M, Li DW, He XM, Weng YQ (2011) Fine genetic mapping of cp: a recessive gene for compact (dwarf) plant architecture in cucumber, Cucumis sativus L. Theor Appl Genet 123:973–983
  • Muangprom A, Osborn TC (2004) Characterization of a dwarf gene in Brassica rapa, including the identification of a candidate gene. Theor Appl Genet 108:1378–1384
  • Multani DS, Briggs SP, Chamberlin MA, Blakeslee JJ, Murphy AS, Johal GS (2003) Loss of an MDR transporter in compact stalks of maize br2 and sorghum dw3 mutants. Science 302:71–72
  • Nakata M, Yuasa T, Takahashi Y, Ishida S (2009) CDPK1, a calcium dependent protein kinase, regulates transcriptional activator RSG in response to gibberellins. Plant Signal Behav 4(5):372–374
  • Noctor G (2006) Metabolic signalling in defence and stress: the central roles of soluble redox couples. Plant Cell Environ 29(3):409–425
  • Otani M, Meguro S, Gondaira H, Hayashi M, Saito M, Han DS, Inthima P, Supaibulwatana K, Mori S, Jikumaru Y, Kamiya Y, Li T, Niki T, Nishijima T, Koshioka M, Nakano M (2013)
  • Overexpression of the gibberellin 2-oxidase gene from Torenia fournieri induces dwarf phenotypes in the liliaceous monocotyledon Tricyrtis sp. J Plant Physiol 170(16):1416–1423
  • Pacheco-Villalobos D, Sankar M, Ljung K, Hardtke CS (2013) Disturbed local auxin homeostasis enhances cellular anisotropy and reveals alternative wiring of auxin-ethylene crosstalk in Brachypodium distachyon seminal roots. PLoS Genet 9(6):e1003564
  • Peng J, Richards DE, Hartley NM, Murphy GP, Devos KM, Flintham JE, Beales J, Fish LJ, Worland AJ, Pelica F, Sudhakar D, Christou P, Snape JW, Gale MD, Harberd NP (1999) ‘Green revolution’ genes encode mutant gibberellin response modulators. Nature 400:256–261
  • Qi J, Liu X, Shen D, Miao H, Xie B, Li X, Zeng P, Wang S, Shang Y, Gu X, Du Y, Li Y, Lin T, Yuan J, Yang X, Chen J, Chen H, Xiong X, Huang K, Fei Z, Mao L, Tian L, Städler T, Renner SS, Kamoun S, Lucas WJ, Zhang Z, Huang S (2013) A genomic variation map provides insights into the genetic basis of cucumber domestication and diversity. Nat Genet 45(12):1510–1515
  • Qiao H, Chang KN, Yazaki J, Ecker JR (2009) Interplay between ethylene, ETP1/ETP2 F-box proteins, and degradation of EIN2 triggers ethylene responses in Arabidopsis. Genes Dev 23:512–521
  • Ranathunge K, Kotula L, Steudle E, Lafitte R (2004) Water permeability and reflection coefficient of the outer part of young rice roots are differently affected by closure of water channels (aquaporins) or blockage of apoplastic pores. J Exp Bot 55:433–447
  • Ren XF, Sun DF, Guan WW, Sun GL, Li CD (2010) Inheritance and identification of molecular markers associated with a novel dwarfing gene in barley. BMC Genet 11:89
  • Richards DE, King KE, Ait-Ali T, Harberd NP (2001) How gibberellin regulates plant growth and development: a molecular genetic analysis of gibberellin signaling. Ann Rev Plant Physiol Plant Mol Biol 52:67–88
  • Roberts IN, Lloyd CW, Roberts K (1985) Ethylene induced microtubule reorientations: mediation by helical arrays. Planta 116:439–447
  • Sasaki A, Itoh H, Gomi K, Ueguchi-Tanaka M, Ishiyama K, Kobayashi M, Jeong DH, An G, Kitano H, Ashikari M, Matsuoka M (2003) Accumulation of phosphorylated repressor for gibberellin signaling in an F-box mutant. Science 299:1896–1898
  • Schulz B, Kolukisaoglu HU (2006) Genomics of plant ABC transporters: the alphabet of photosynthetic life forms or just holes in membranes? FEBS Lett 580(4):1010–1016
  • Shen S, Sharma A, Komatsu S (2003) Characterization of proteins responsive to gibberellin in the leaf-sheath of rice (Oryza sativa L.) seedling using proteome analysis. Biol Pharm Bull 26(2):129–136
  • Shi YS, Yu YT, Song YC, Liu ZZ, Li Y, Wang TY (2010) Identification of a QTL for plant height in a new dwarf germplasm of maize. Acta Agron Sin 36(2):256–260
  • Sugawara H, Shibuya K, Yoshioka T, Hashiba T, Satoh S (2002) Is a cysteine proteinase inhibitor involved in the regulation of petal wilting in senescing carnation (Dianthus caryophyllus L.) flowers. J Exp Bot 53:407–413
  • Szekeres M, Németh K, Koncz-Kálmán Z, Mathur J, Kauschmann A, Altmann T, Rédei GP, Nagy F, Schell J, Koncz C (1996) Brassinosteroids rescue the deficiency of CYP90, a cytochrome P450, controlling cell elongation and de-etiolation in Arabidopsis. Cell 85(2):171–182
  • Tar’an B, Warkentin T, Somers DJ, Miranda D, Vandenberg A, Blade S, Woods S, Bing D, Xue A, Dekoeyer D, Penner G (2003) Quantitative trait loci for lodging resistance, plant height and partial resistance to mycosphaerella blight in field pea (Pisum sativum L.). Theor Appl Genet 107:1482–1491
  • Ueguchi-Tanaka M, Ashikari M, Nakajima M et al (2005) GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin. Nature 437:693–698
  • Ueguchi-Tanaka M, Nakajima M, Motoyuki A, Matsuoka M (2007) Gibberellin receptor and its role in gibberellin signaling in plants. Annu Rev Plant Biol 58:183–198
  • Varsano T, Wolf SG, Pick U (2006) A chlorophyll a/b-binding protein homolog that is induced by iron deficiency is associated with enlarged photosystem I units in the eucaryotic alga Dunaliella salina. J Biol Chem 281(15):10305–10315
  • Vierstra RD (2009) The ubiquitin- 26S proteasome system at the nexus of plant biology. Nat Rev Mol Cell Biol 10:385–397
  • Wang Q, Wen B, Yan G, Wei J, Xie L, Xu S, Jiang D, Wang T, Lin L, Zi J, Zhang J, Zhou R, Zhao H, Ren Z, Qu N, Lou X, Sun H, Du C, Chen C, Zhang S, Tan F, Xian Y, Gao Z, He M, Chen L, Zhao X, Xu P, Zhu Y, Yin X, Shen H, Zhang Y, Jiang J, Zhang C, Li L, Chang C, Ma J, Yan G, Yao J, Lu H, Ying W, Zhong F, He QY, Liu S (2013) Qualitative and quantitative expression status of the human chromosome 20 genes in cancer tissues and the representative cell lines. J Proteome Res 12(1):151–161
  • Ward SP, Leyser O (2004) Shoot branching. Curr Opin Plant Biol 7:73–78
  • Wrzaczek M, Brosché M, Salojärvi J, Kangasjärvi S, Idänheimo N, Mersmann S, Robatzek S, Karpiński S, Karpińska B, Kangasjärvi J (2010) Transcriptional regulation of the CRK/DUF26 group of receptor-like protein kinases by ozone and plant hormones in Arabidopsis. BMC Plant Biol 10:95
  • Xing S, Rosso MG, Zachgo S (2005) ROY1, a member of the plant glutaredoxin family, is required for petal development in Arabidopsis thaliana. Development 132(7):1555–1565
  • Xu ZF, Chye ML, Li HY, Xu FX, Yao KM (2003) G-box binding coincides with increased Solanum melongena cysteine proteinase expression in senescent fruits and circadian-regulated leaves. Plant Mol Biol 51:9–19
  • Yamori W, Takahashi S, Makino A, Price GD, Badger MR, von Caemmerer S (2011) The roles of ATP synthase and the cytochrome b6/f complexes in limiting chloroplast electron transport and determining photosynthetic capacity. Plant Physiol 155(2):956–962
  • Yang G, Komatsu S (2004) Microarray and proteomic analysis of brassinosteroid- and gibberellin-regulated gene and protein expression in rice. Genomics Proteomics Bioinform 2(2):77–83
  • Yazaki J, Shimatani Z, Hashimoto A, Nagata Y, Fujii F, Kojima K, Suzuki K, Taya T, Tonouchi M, Nelson C, Nakagawa A, Otomo Y, Murakami K, Matsubara K, Kawai J, Carninci P, Hayashizaki Y, Kikuchi S (2004) Transcriptional profiling of genes responsive to abscisic acid and gibberellin in rice: phenotyping and comparative analysis between rice and Arabidopsis. Physiol Genomics 17(2):87–100
  • Yu XC, Li MJ, Gao GF, Feng HZ, Geng XQ, Peng CC, Zhu SY, Wang XJ, Shen YY, Zhang DP (2006) Abscisic acid stimulates a calcium-dependent protein kinase in grape berry. Plant Physiol 140:558–579
  • Yuan HM, Chen S, Lin L, Wei R, Li HY, Liu GF, Jiang J (2012) Genome-wide analysis of a TaLEA-Introduced transgenic Populus simonii 9 Populus nigra dwarf mutant. Int J Mol Sci 13(3):2744–2762
  • Yuan SJ, Wang T, Yin L, Zhao JF, Wan JM, Li XY (2013) Positional cloning and expression analysis of gene responsible for hightillering dwarf phenotype in indica rice mutant gsor23. Rice Sci 20(4):320–328
  • Zhang BS, Tian F, Tan LB, Xie DX, Sun CQ (2011) Characterization of a novel high-tillering dwarf3 mutant in rice. J Genet Genom 38(9):411–418
  • Zhou L, Zeng Y, Zheng W, Tang B, Yang S, Zhang H, Li J, Li Z (2010) Fine mapping a QTL qCTB7 for cold tolerance at the booting stage on rice chromosome 7 using a near-isogenic line. Theor Appl Genet 121(5):895–905
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