Emergence of high-level gentamicin resistance among Enterococci clinical isolates from burn patients in south-west of Iran: vancomycin still working

• Autorzy: Labibzadeh M. , Kaydani G.A. , Savari M. , Ekrami A.
• Języki publikacji: EN

Abstrakty

EN

Enterococcus faecalis and Enterococcus faecium are among the main agents associated with nosocomial infections with high mortality in immunocompromised patients. Antibiotic resistance, especially against gentamicin and vancomycin among Enterococci, is a risk factor that could increase the morbidity and mortality rate. 179 Enterococci isolates from burn patients were included in this study. Antibiotic susceptibility testing was done using the disk diffusion test and minimum inhibitory concentration (MIC) was evaluated by agar microdilution. Vancomycin and gentamicin resistance associated genes including vanA, vanB, vanC, aac (6’)-Ie aph(2’’), aph(3’)-IIIa and ant(4’)-Ia were detected by PCR and their statistical relation with antibiotic resistance was evaluated. E. faecalis was the more prevalent strain among our local isolates and showed a higher antibiotic resistance in comparison to E. faecium. Vancomycin had a good antibacterial effect on the Enterococcus spp. isolates; however, resistance to this antibiotic and a high-level gentamicin resistance (HLGR) phenotype were observed. Among van operon genes, vanA was the most prevalent gene and among the gentamicin resistance genes, aph (3’)-IIIa was more frequent. The HLGR Enterococci are a real challenge in nosocomial infections. Vancomycin is a key antibiotic to treat such infections but emergence of VRE in our region could be a real concern and, therefore, phenotypic and molecular surveillance must be considered.

Słowa kluczowe

EN

gentamicin; drug resistance; enterococcal infection; bacterial infection; Enterococcus faecalis; Enterococcus faecium; risk factor; clinical isolate; burn; patient; vancomycin; Iran

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2018
• Tom: 67
• Numer: 4
• Opis fizyczny: p.401-406,fig.,ref.

Twórcy

autor

Labibzadeh M.

• Department of Clinical Laboratory Sciences, School of Paramedicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

autor

Kaydani G.A.

• Department of Clinical Laboratory Sciences, School of Paramedicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

autor

Savari M.

• Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

autor

Ekrami A.

• Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

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Identyfikatory

DOI

10.21307/pjm-2018-043