First laboratory confirmation of salmonid alphavirus type 2 (SAV2) infection in Poland

• Autorzy: Borzym E. , Maj-Paluch J. , Stachnik M. , Matras M. , Reichert M.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to identify the genotype of Polish isolates of salmonid alphaviruses (SAV) and to find the origin of the virus. Samples for virus isolation included the kidneys, spleen, and liver pooled from 10 fish. A typical cytopathic effect was observed after inoculation of samples on cell lines. Total RNA was extracted from cell culture supernatant and submitted to RT-PCR with primers amplifying two informative regions of the genome: a conserved region in the E2 gene and a variable region in the nsP3 gene. The sequences revealed that the strain from Poland belonged to subtype SAV 2, indicating a very strong genetic identity with isolates from Italy and France.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2014
• Tom: 58
• Numer: 3
• Opis fizyczny: p.341-345,fig.,ref.

Twórcy

autor

Borzym E.

• Department of Fish Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Maj-Paluch J.

• Department of Fish Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Stachnik M.

• Department of Fish Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Matras M.

• Department of Fish Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Reichert M.

• Department of Fish Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

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Identyfikatory

DOI

10.2478/bvip-2014-0053