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2015 | 37 | 01 |
Tytuł artykułu

Identification of salt-tolerant barley varieties by a consolidated physiological and molecular approach

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Soil salinity is a significant international environmental problem and severely affects the yield of cereal crops due to numerous effects on plant–water relations, ion homeostasis and salt toxicity. This work assessed the salt tolerance levels of 16 barley varieties by key physiological and molecular tests. Seedlings were exposed to acute salinity stress and their physiological responses including relative water content, levels of the stress hormone abscisic acid (ABA) and Na?/K? ratio were compared. A significant variation was noted amongst the varieties, with Calmariout, Hindmarsh and Mundah identified as tolerant varieties and Franklin as the most sensitive variety. Further, the differential expression of key genes in the ABAregulated stress response pathway, i.e., pyrabactin resistance (PYR)/PYR-Like (PYL)/regulatory components of ABA receptors (RCAR), protein phosphatase 2C (PP2C), sucrose non-fermenting 1-related kinase (SnRK2) and specific ABC transporters, was analysed by quantitative real-time PCR of leaf RNA. The PP2CA and ABC transporters were up-regulated while the PYR/PYL/RCARs and SnRK2s were down-regulated under salt stress. Importantly, tolerance ranking by gene expression closely correlated that by physiological indices. Thus expression analysis of the ABA pathway may be used for rapid identification of potentially salt-tolerant barley varieties before undertaking physiological studies. The outcomes are relevant for varietal selection and comparing the alleles for genetics of tolerance.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
37
Numer
01
Opis fizyczny
Article: 1716 [12 p.], fig.,ref.
Twórcy
autor
  • Faculty of Science, Engineering and Technology, Swinburne University of Technology, PO Box 218, Hawtborn, WIC 3122, Australia
autor
  • Baker IDI Heart and Diabetes Institute, 75 Commercial Road, Melbourne, VIC 3004, Australia
autor
  • Faculty of Science, Engineering and Technology, Swinburne University of Technology, PO Box 218, Hawtborn, WIC 3122, Australia
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-3b7a5f65-9a3c-4e3c-931e-741aed7d2b50
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