Evaluation of anesthesia and reproductive performance upon diazepam and xylazine injection in rats

• Autorzy: Abd El-Nasser A. Mohammed , A.Abdelnabi M. , Modlinski J. A.
• Języki publikacji: EN

Abstrakty

EN

The aims of the current study were to evaluate the effects of diazepam (D) and/or xylazine (X) on the induction of sedation and anesthesia, performing ovarian transplantation and litter size upon administration during pregnancy. One hundred and five albino rat females were classified randomly into four groups given intraperitoneal (IP) injection of physiological saline (group I), diazepam (6.2 mg /kg body weight - group II), xylazine (13.2 mg/kg/body weight - group III) and 6.2 mg/kg body weight diazepam and 13.2 mg/kg body weight xylazine (group IV). Induction of sedation and anesthesia were monitored. The onset of anesthesia, duration of surgical anesthesia and sleep time after injections was recorded. Rectal temperatures were monitored at 0, 20, 40, 60 and 120 min after injection. Blood samples were collected at 0 and 120 min post injection for determination of hemoglobin, glucose and urea concentrations. Ovarian transplantation was performed in females anesthesized with DX drugs. Mated females were injected with physiological saline, 6.2 mg/kg diazepam and/or 13.2 mg/kg xylazine at day 4, 11 and 18 of pregnancy and litter sizes were recorded. Sedation was obtained after injection of diazepam or xylazine alone whereas surgical anesthesia was obtained upon injection of both diazepam and xylazine. The onset of anesthesia, duration of anesthesia and sleep time after DX injection was 4.6, 144.1 and 79.1 min, respectively.Rectal temperature decreased (P<0.05) after injection of diazepam and/or xylazine. The hemoglobin (P<0.05), glucose and urea blood concentrations increased after injection of diazepam and/or xylazine. Upon ovarian transplantation, all animals recovered and ovarian follicles were found In the transplanted ovaries. Number of offspring per animal did not differ due to diazepam and/or xylazine injection at day 4, 11 and 18 of pregnancy. In conclusion, DX injection induced surgical anesthesia in rats sufficient for performing major surgeries.

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2012
• Tom: 30
• Numer: 3
• Opis fizyczny: p.285-292,fig.,ref.

Twórcy

autor

Abd El-Nasser A. Mohammed

• Department of Animal and Poultry Production, Faculty of Agriculture, University of Assiut, Assiut 71526, Egypt

autor

A.Abdelnabi M.

autor

Modlinski J. A.

Bibliografia

• AVERILL D.,R., Jr., 1970- Treatment of status epilepticus in dogs with diazepam sodium. Journal of the American Veterinary Medical Association 56, 432-434.
• BORKOWSKI G., L., DANNEMAN P., RUSSELL G.,B., LANG C., M., 1990- An evaluation of three intravenous anesthetic regimens in New Zealand rabbits. Laboratory Animal Science 40, 270-276.
• BRIGHT, R.M., 1986- Surgical emergencies. First edition. Churchill, USA, pp: 51.
• CUSTER R., KRAMER L., KENNEDY S., BUSH M., 1977- Hematologic Effects of Xylazine When Used for Restraint of Bactrian Camels. Journal of The American Veterinary Medical Association 171 (9), 899-901.
• DWIVEDI R.K., SHARMA S.P., 2004- Indian Journal of Veterinary and Surgery 25 (1), 42-43.
• DOBRINSKI, I.; LULAI C., BARTH A.D., 1994- Effects of xylazine on early bovine pregnancy. Animal Reproduction Science 36, 25-36.
• DORSCH, M., WEDEKIND D., KAMINO K., HEDRICH H.J., 2004 - Orthotopic transplantation of rat ovaries as a tool for strain rescue. Laboratory Animals 38, 307-312.
• FANI F.A., MEHESARE1 S.P., PAWSHE D.B., KHAN K.M., JADHAV N.D., 2004 – Haematological and Biochemical changes during Epidural Xylazine hydrochloride anaesthesia in Dogs. Veterinary World 1 (6), 175-177.
• FLECKNELL P.A., JOHN M., MITCHELL M., SHUREY C., SIMPKIN S., 1983 – Neuroleptanalgesia in the rabbit. Laboratory Animals 17, 104-109.
• FLECKNELL P., - 2003. Laboratory Animal Anesthesia, 2nd Edition. Elsevier, 2003.
• GHURASHI M.A.H., SERI H.I., BAKHEIT A.H., ASHWAG E.A.M., ABAKAR J.A., 2009-Evaluation of Ketamine/diazepam Anaesthesia for Performing Surgery in Desert.Goats under Field Condition. Australian Journal of Basic and Applied Sciences, 3(2), 455-459
• HALL L.W., CLARKE K.W., TRIM C.M., 2001- Veterinary anaesthesia. 10th edition. W.B. Saunders Co, London.
• HODGSON D.S., DUNLOP C.I., CHAPMAN P.L., SMITH J.A., 2002 - Cardiopulmonary effects of xylazine and acepromazine in pregnant cows in late gestation. American Journal of Veterinary Research 63(12),1695-1699.
• HOFF J., 2000 - Methods of Blood Collection in the Mouse. Laboratory Animals 29 (10), 47-53.
• http://www.patientsville.com/labels/diazepam_label.htm (?)
• JACOBI J., FRASER G., COURSIN D., 2002 - Clinical practice guidelines for the sustained use of sedatives and analgesics in the critically ill adult. Critical Care Medicine 30,119-41.
• LUMB W.E., JONES E.W., 1997- Veterinary anaesthesia, Lea and Febriqer, Philadelphia.
• MOHAMMED A.A., SAYED M.A., ABDELNABI M.A., 2011- New protocol of anesthesia Rusing thiopental, diazepam and xylazine in white New Zealand rabbits. Australian Journal of Basic and Applied Sciences (submitted for publication)
• MUIR, W.W., MASON D.E., 1993 - Effect of diazepam, acepromazine, detomidine and xylazine on thiamylal anaesthesia in horses. Journal of the American Veterinary Medical Association 203(7),1031-1038.
• PEETERS, M.E., GIL D., TESKE E., EYZENBACH V., VD BROM W.E., LUMEIJ J.T., DE VRIES H.W., 1988 - Four methods for general anaesthesia in the rabbit: a comparative study. Laboratory Animals 22, 355-360.
• SAS, 1999 - SAS User’s Guide: Version 6.12. SAS Inst. Inc., Cary, NC.
• SARNOWSKA, A., BERĘSEWICZ M., ZABŁOCKA B., DOMAŃSKA-JANIK K., 2009 -Diazepam neuroprotection in excitotoxic and oxidative stress involves a mitochondrial mechanizm additional to the GABAAR and hypothermic effects. Neurochemistry International 55(1-3), 164-173.
• SYMONDS H.W., 1976 - The Effect of Xylazine Upon Hepatic Glucose Production and Blood Flow Rate in the Lactating Dairy Cow. Veterinary Record 99, 234-236.
• VAN GEIJN, H.P., JONGSMA H.W., DOESBURG W.H., LEMMENS W.A.J.G., DE HAAN J.,ESKES T.K.A.B., 1980 - The effect of diazepam administration during pregnancy or labor on the heart rate variability of the newborn infant. European Journal of Obstetrics & Gynecology and Reproductive Biology 10 (3), 187-201.
• YADAV G.U., THORAT M.G., BEDARKAR S.N., 2008 - Efficacy of Xylazine as a Sedative In Cattle. Veterinary World 1(11), 340.

Uwagi

PL

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