Stress-induced changes in gene expression of catecholamine enzymes and adrenoceptors in heart of sham-operated and PVN-deafferentated rats

• Autorzy: Bohacova V. , Mravec B. , Laukova M. , Kvetnansky R.
• Języki publikacji: EN

Abstrakty

EN

The paraventricular nucleus of the hypothalamus (PVN) regulates neuroendocrine, autonomic and cardiovascular responses to stress. Stressors activate catacholaminergic systems which consequently modulates activity of organism via adrenergic receptors (ARs). Our work was focused on investigation of changes in gene expression of epinephrine-synthesizing enzyme phenylethanolamine-N-methyltransferase (PNMT), neuropeptide Y (NPY) and adrenoceptors in the heart of sham-operated and PVN-deafferentated rats exposed to immobilization stress (IMO). PNMT and NPY mRNA levels in the heart of sham-operated rats were not signifi cantly increased after a single IMO. However, posterolateral deafferentation of the PVN reduced PNMT mRNA, whereas levels of NPY mRNA were elevated. These data suggest a stimulatory role of PVN on PNMT and an inhibitory role on NPY gene expression. Moreover, β1-AR mRNA levels were signifi cantly reduced in heart of PVN-deafferentated animals comparing to sham-operated rats. On other side, after single IMO β1-AR mRNA levels were signifi cantly increased in PVN-deafferentated rats. Gene expression of other ARs (β2, β3, α1B) in hearts of stressed PVN-deafferentated animals were decreased. The found down-regulation of gene expression of ARs might suggest a rise in sympathetic activity induced by PVN deafferentation. Our data suggest that PVN plays an important role in stress-induced activation of cardiac sympathetic system.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 3
• Opis fizyczny: p.357

Twórcy

autor

Bohacova V.

• Laboratory of Stress Research, Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic

autor

Mravec B.

• Laboratory of Functional Morphology, Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic

autor

Laukova M.

• Laboratory of Stress Research, Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic

autor

Kvetnansky R.

• Laboratory of Stress Research, Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic