Wpływ preparatu Cyperkill Super 25 EC na aktywność katalazy w komórkach drożdży Saccharomyces cerevisiae

• Autorzy: Krzepilko A. , Swiecilo A.

Warianty tytułu

EN

Effect of cypermethrin - Cyperkill Super 25 EC active compound on the catalase activity of Saccharomyces cerevisiae yeast

• Języki publikacji: PL

Abstrakty

PL

Badano wpływ insektycydu Cyperkill Super 25 EC na przeżywalność komórek drożdży. Zmiany aktywności katalazy przyjęto jako wskaźnik intensywności odpowiedzi stresowej. Cypermetryna - aktywny składnik preparatu Cyperkill Super 25 EC - o stężeniach 175-325 µg·cm⁻³ powodowała spadek przeżywalności komórek drożdży w logarytmicznej fazie wzrostu. W komórkach inkubowanych z cypermetryczną nie stwierdzono przyrostu aktywności katalazy. Aktywność katalazy w komórkach poddanych dwu różnym stresom równocześnie (stres termiczny i cypermetryna lub stres osmotyczny i cypermetryna) była zbliżona do kontroli. Wyniki sugerują, że cypermetryna hamuje indukowaną syntezę katalazy w warunkach stresu termicznego lub osmotycznego.

EN

The effect of insecticide on the survival of yeast Saccharomyces cerevisiae was investigated. Cypermethrin - Cyperkill Super 25 EC active compound at doses of 175-325 µg·cm⁻³ caused a decrease in viability of yeast cells in logarithmic phase of growth. The activity of catalase was chosen as an indicator of stress response. The results did not show the increase of catalase activity in cypermethrin treated yeast. It was observed that two kinds of stress (heat shock and cypermethrin, osmotic stress and cypermethrin) applied at the same time had no additive effects. The activity of catalase under these conditions was the same as in control. These results suggested that cypermethrin inhibited catalase induction in yeast in stress conditions.

• Wydawca: -
• Czasopismo: Zeszyty Problemowe Postępów Nauk Rolniczych
• Rocznik: 2004
• Tom: 496
• Numer: 2
• Opis fizyczny: s.477-485,tab.,bibliogr.

Twórcy

autor

Krzepilko A.

• Zakład Biochemii, Instytut Nauk Rolniczych, Akademia Rolnicza w Lublinie, ul.Szczebrzeska 102, 22-400 Zamość

autor

Swiecilo A.

• Zakład Biochemii, Instytut Nauk Rolniczych, Akademia Rolnicza w Lublinie, ul.Szczebrzeska 102, 22-400 Zamość

Bibliografia

• Akhtar N., Kayani S.A., Ahmad M.M., Shahab M. 1996. Insecticide- induced changes in sectory activity of the thygroid gland in rats. J. Appl. Toxicol. 16(5): 397-400.
• Aldana L., Gonzales de Mejia E., Craigmill A., Tsutsumi V., Armendariz-Borunda J., Panduro A., Rincon A. 1998. Cypermethrin increases apo A-l and apo-B mRNA but not hyperlipidemia in rats. Toxicol. Lett. 16: 31-39.
• Amer S.H., Aboul-ela E.I. 1985. Cytogenetic effects of pesticides. III. Induction of micronuclei in mouse bone marrow by the insecticides cypermethrin and rotenone. Mutation Res. 155: 135-142.
• Beers R.F., Sizer J.W. 1952. A spectrophotometric method of measuring the breakdown of hydrogen peroxide by catalase. J. Biol. Chem. 195: 133-140.
• Belazzi T., Wagner A., Wieser R., Schanz M., Adam G., Hartig A., Ruis H. 1991. Negative regulation of transcription of the Saccharomyces cerevisiae catalase T (CTT1) gene by cAMP is mediated by a positive control element. EMBO J. 10(3): 585-592.
• Biliński T., Krawiec Z., Liczmański A., Litwińska J. 1985. Is hydroxyl radical generated by the Fenton reaction in vivo? Biochem. Biophys. Res. Commun. 130(2): 533-539.
• Błaszczyński M., Litwińska J., Zaborowska D., Biliński T. 1985. The role of respiratory chain in paraquat toxicity in yeast. Acta Microbiol. Pol. 34(3/4): 243-254.
• Das B.K., Mukherjee S.C. 2003. Toxicity of cypermeehrin in Labeo rohita fingeerlings: biochemical, enzymatic, and haematological consequences. Comparative Biochemistry and Physiology, Part C, 134: 109-121.
• El-Tawil O., Abdel-Rahman M. 2001. The role of enzyme induction and inhibition on cypermethrin hepatotoxicity. Pharmacological Research 44(1): 33-39.
• Giray B., Gurbay A., Hincal F. 2001. Cypermethrin - induced oxidative stress in rat brain and liver is prevented by vitamin E or allopurinol. Toxicol. Lett. 118(3): 139-146.
• Imamura L., Hasegawa H., Kurashina K., Hamanishi A., Tabuchi A., Tsuda M. 2000. Repression of activity-dependent c-fos and brain-derived neurotrophic factor mRNA expression by pyrethroid insecticides accompanying a decrease in Ca²⁺ influx into neurons. Pharmacol. Exp. Ther. 295(3): 1175-82.
• Kakko I., Toimela T., Tahti H. 2003. The synaptosomal membrane bound ATPase as a target for the neurotoxic effects od pyrethroids, permethrin and cypermethrin. Chemosphere 51: 475-480.
• Kale M., Rathore N., Deepak B. 1999. Lipid peroxidation and antioxidant enzymes in rat tissues in pyrethroid toxicity: possible involvement of reactive oxygen species. J. Nutrit. Environ. Medicine 9(1): 37-47.
• Kale M., Rathore N. 1999. The protective effect of vitamin E in pyrethroid-induced oxidative stress in rat tissues. J. Nutrit. Environ. Medicine 9(4): 281-228.
• Kobayashi N., McEntee K. 1993. Identification of cis and trans components of a novel heat shock stress regulatory pathway in Saccharomyces cerevisiae. Mol. Cell Biol. 13(1): 248-256.
• Krawiec Z. 1999. Stress response in yeast cells to the herbicide N-(phosphomethyl) glycine. Acta Physiol. Plant. 21(3) supplement: 50.
• Krawiec Z., Biliński T. 1987. New types of catalase regulatory mutants in yeast. Bull. Pol. Acad. Sci. Biol., Ser. B 35: 285-291.
• Krawiec Z., Biliński T., Schüller Ch., Ruis H. 2000. Reactive oxygen species as second messengers? Induction of the expression of yeast catalase T gene by heat and hyperosmotic stress does not require oxygen. Acta. Biochim. Polon. 47(1): 201-207.
• Lowry O., Rosebrough W., Farr A.L., Randall R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275.
• Marchler G., Schüller C., Adam G., Ruis H. 1993. A Saccharomyces cerevisiae UAS element controlled by protein kinase A activates transcription in response to a variety of stress conditions. EMBO J. 12(5): 1997-2003.
• Mukhopadhyay I., Nazir A., Saxena D.K., Chowdhuri D.K. 2002. Toxicity of cypermethrin: hsp70 as a biomarker of response in transgenic Drosophila. Biomarkers 7(6): 501-10.
• Oktaba W. 1997. Elementy statystyki matematycznej i metodyka doświadczalnictwa. PWN Warszawa: 310 ss.
• Philip G.H., Rajasree B.H. 1996. Action of cypermethrin on tissue transamination during nitrogen metabolism in Cyprinus carpio. Ecotoxicology and Environmental Safety 34: 174-179.
• Różański L. 1992. Przemiany pestycydów w organizmach żywych i środowisku. PWRiL Warszawa: 381 ss.
• Różański L. 1996. Vademecum pestycydów. Agra-Enviro Lab. Poznań: 327 ss.
• Ruis H. 1979. The biosynthesis of catalase. Can. J. Biochem. 57(9): 1122-1130.
• Ruis H., Schüller G. 1995. Stress signaling in yeast. Bioessays 17(11): 959-965.
• Sies H., Cadenas E. 1985. Oxidative stress: damage to intact cells and organs. Philosoph. Transac. Royal Soc. London, Ser. B-Biol. Sci. 17(311): 617-31.
• Surralles J., Xamena N., Creus A., Catalan J., Norppa H., Marcos R. 1994. Induction of micronuclei by five pyrethroid insecticides in whole-blood and isolated human lymphocyte cultures. Mutation Res. 341: 169-184.
• Swan T.M., Watson K. 1999. Stress tolerance in a yeast lipid mutant: membrane lipids influence tolerance to heat and ethanol independently of heat shock proteins and trehalose. Can. J. Microbiol. 45(6): 427-479.
• Uner N., Oruc E.O., Canli M., Sevgiler Y. 2001. Effects of cypermethrin on antioxidant enzyme activities and lipid peroxidation in liver and kidney of the freshwater fish, Oreochromis niloticus and Cyprinus carpio (L.). Bull. Environ. Contam. Toxicol. 67(5): 657-64.
• Vontas J.G., Small G.J., Hemingway J. 2001. Glutathione S-transferases as antioxidant defence agents confer pyrethroid resistance in Nilaparvata lugens. Biochem. J. 357(1): 65-72.
• Wieser R., Adam G., Wagner A., Schuller C., Marchler G., Ruis H., Krawiec Z., Biliński T. 1991. Heat shock factor-independent heat control of transcription of the CTT1 gene encodingthe cytosolic catalase T of Saccharomyces cerevisiae. J. Biol. Chem. 266: 12406-12411.
• Woollen B.H., Marsh J.R., Laird W.J.D. Lesser J.E. 1992. The metabolism of cypermethrin in man: differences in urinary metabolite profiles following oral and dermal administration. Xenobiotica 22: 983-991.