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2012 | 17 | 2 |

Tytuł artykułu

Maintenance of rat hepatocytes under inflammation by coculture with human orbital fat-derived stem cells

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Preservation of hepatocyte functions in vitro will undoubtedly help the management of acute liver failure. The coculture system may be able to prevent functional decline of hepatocytes. It has already been shown that hepatocytes, when cocultured with bone marrow mesenchymal stem cells, could undergo long-term culture in vitro without loss of functions. In this study, human orbital fat-derived stem cells were isolated and cocultured with rat hepatocytes. When treated with serum from an acute liver failure patient, rat hepatocyte monoculture showed reduction of cell viability and loss of liverspecific functions. However, rat hepatocytes in the coculture system were still able to secret albumin and synthesize urea. IL-6 was significantly elevated in the coculture of rat hepatocyte with orbital fat-derived stem cells, and it might be the key immunoregulator which protects rat hepatocytes against inflammation. Our data confirmed that orbital fat-derived stem cells, or other adipose tissue-derived stem cells, are an ideal candidate to support rat hepatocyte functions in vitro.

Wydawca

-

Rocznik

Tom

17

Numer

2

Opis fizyczny

p.182-195,fig.,ref.

Twórcy

autor
  • Department of Infectious Disease, Southwest Hospital Affiliated to Third Military Medical University, Chongqing 400038, China
autor
autor
autor
autor

Bibliografia

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Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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