Reactive oxygen species release, Vitamin E, fatty acid and phytosterol contents of artificially aged radish (Raphanus sativus L.) seeds during germination

• Autorzy: Bellani L.M. , Salvini L. , Dell'Aquila A. , Scialabba A.
• Języki publikacji: EN

Abstrakty

EN

Seeds of Raphanus sativus L. subjected to accelerated ageing were investigated for reactive oxygen species (ROS) release and for content of vitamin E (tocopherol, TOC, and tocotrienol, TOC-3), fatty acids and phytosterols in seed coats, cotyledons and embryonic axes during germination. In unaged seeds, ROS release occurred mainly in seed coats of non-imbibed seeds and in seedlings (48 h of imbibition). TOC and TOC-3 were mainly represented by the γ-isoform, abundant in embryonic axes. Fatty acids were mainly found in cotyledons. In seed coat and embryonic axis, phytosterols consisted mainly of sitosterols. The effects of ageing were mainly visible in embryonic axes at 48 h of imbibition. Deterioration was associated with a decrease in fresh weight increase percentage, germination percentage, α-TOC and total fatty acid content. An increase in ROS release from seed coats and in γ-TOC, δ-TOC, γ-TOC-3 content in embryonic axis was also observed. The use of γ-TOC and total fatty acids in embryonic axis as parameters of seed quality evaluation during storage was suggested.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 5
• Opis fizyczny: p.1789-1799,fig.,ref.

Twórcy

autor

Bellani L.M.

• Dipartimento di Biologia Evolutiva, Universita di Siena, 53100 Siena, Italy

autor

Salvini L.

• Centro di Analisi e Determinazioni Strutturali, Universita di Siena, 53100 Siena, Italy
• Toscana Life Sciences Founation, Via Fiorentina 1, 53100 Siena, Italy

autor

Dell'Aquila A.

• Institute of Plant Genetics, CNR, 70126 Bari, Italy

autor

Scialabba A.

• Laboratorio Sistema Madonie, Dipartimento di Biologia Ambientale e Biodiversita, Universita di Palermo, 90123 Palermo, Italy

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