Oxidative stress-mediated cytotoxicity of cadmium in chicken splenic lymphocytes

• Autorzy: Li J.-L. , Li H.-X. , Li S. , Tang Z.-X. , Xu S.-W. , Wang X.-L.
• Języki publikacji: EN

Abstrakty

EN

Cadmium (Cd), a potent immunotoxicant, has been reported to affect immunocytes both in humans and in rodents. However, whether such an effect exists in birds is still unclear. To delineate the cytotoxicity of Cd on the bird immunocytes in vitro, chicken splenic lymphocytes were exposed to Cd. A significant increase in the occurrence of cell damage was observed in chicken splenic lymphocytes following Cd administration. The enhanced level of LPO and the overproduction of ROS suggested that Cd caused oxidative stress in chicken splenic lymphocytes. A pronounced inhibition of SOD and GPx was seen after being indicated with 10 μM Cd for 36h or indicated with 15 μM Cd for 24 h. In addition, DNA damage and apoptosis was observed in chicken splenic lymphocytes treated with Cd. The degree of DNA damage and the number of apoptotic cells rose in a time- and dose-dependent manner. These results clearly demonstrate that chicken splenic lymphocytes appear to be more susceptible than mammalian immunocytes to the adverse effects of Cd. The oxidative stress and subsequent DNA damage and apoptosis induced by Cd are important mechanisms of Cd cytotoxicity to bird immunocytes.

Słowa kluczowe

EN

oxidative stress; cytotoxicity; cadmium; chicken; splenic lymphocyte; DNA damage; apoptosis

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2010
• Tom: 19
• Numer: 5
• Opis fizyczny: p.947-956,fig.,ref.

Twórcy

autor

Li J.-L.

• College of Veterinary Medicine, South China Agricultural University, Wushan Road 483, Guangzhou 510642, People’s Republic of China
• College of Veterinary Medicine, Northeast Agricultural University, Mucai Street 59, Harbin 150030, People’s Republic of China

autor

Li H.-X.

• State Key Laboratory of Veterinary Biotechnology, Division of Avian Infectious Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Maduan Street 427, Harbin 150001, People’s Republic of China

autor

Li S.

• College of Veterinary Medicine, Northeast Agricultural University, Mucai Street 59, Harbin 150030, People’s Republic of China

autor

Tang Z.-X.

• College of Veterinary Medicine, South China Agricultural University, Wushan Road 483, Guangzhou 510642, People’s Republic of China

autor

Xu S.-W.

• College of Veterinary Medicine, Northeast Agricultural University, Mucai Street 59, Harbin 150030, People’s Republic of China

autor

Wang X.-L.

• Wildlife Resource College, Northeast Forestry University, Hexing Road 26, Harbin 150040, People’s Republic of China

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