Detection and identification of banned processed animal protein in feedingstuffs by microscopic and PCR methods

• Autorzy: Weiner A. , Golebiowska A. , Paprocka I. , Kwiatek K.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to present the results of comparative evaluation of the usefulness of PCR and microscopic methods for the detection of Processed Animal Protein (PAP) in feedingstuffs. In the validation study, the limit of the detection for PCR was determined on 0.05% for beef, 0.1% for pork and 0.2% for poultry meat and bone meal (MBM). Among 62 doubtful samples of feedingstuffs examined by microscopic method 41 (66.13%) were found as positive. Based on the results obtained with the use of the microscopic and PCR methods it is possible to state that the molecular biology methods can, at present, be used as a supplementary method in PAP detection.

Słowa kluczowe

EN

detection; identification; processed animal protein; microscopic method; polymerase chain reaction; feeding stuff; meat meal; bone meal

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2012
• Tom: 15
• Numer: 1
• Opis fizyczny: p.163-164,ref.

Twórcy

autor

Weiner A.

• Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland

autor

Golebiowska A.

autor

Paprocka I.

autor

Kwiatek K.

Bibliografia

• Regulation (EC) No 1069/2009 of the European Parliament and of the Council of October 2009 laying down health rules as regards animal by-products and derived products not intended for human consumption and repealing Regulation (EC) No. 1774/2002 (animal by-products regulation) (O.J.L 300/1, 14.11.2009).
• Commission Regulation (EC) No 152/2009 of January 2009 laying down the methods of sampling and analysis for the official control of feed (O.J.L 54/1, 26.2.2009).
• Martin I., Garcia T., Fajardo V., López-Calleja I., Hernández P.E., Gonazález I., Martín R. (2007) Species-specific PCR for the identification of ruminant species in feedstuffs. Meat Sci 75:120-127.
• Frezza D., Giambra V., Chegdani F., Fontana C., Maccabiani G., LosioN., Faggionato E., Chiappini B., Vaccari G., von Holst C., Lanni L., Saccares S., Ajmone-Marsan P. (2008) Standard and Light-Cycler PCR methods for animal DNA species detection in animal feedstuffs. Innov Food Sci Emerg Technol 9: 18-23.