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2014 | 36 | 03 |
Tytuł artykułu

Two NAC transcription factors from Citrullus colocynthis, CcNAC1, CcNAC2 implicated in multiple stress responses

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
NAC (no apical meristem, Arabidopsis transcription activation factor 1 and 2, cup-shaped cotyledon 2) transcription factors (TFs) play important roles in plant growth, development, and responses to abiotic and biotic stress. Two novel NAC TFs were isolated from Citrullus colocynthis, a highly drought-tolerant cucurbit species: CcNAC1 and CcNAC2 each with conserved A–E NAC domains. Subcellular location of CcNAC1 and CcNAC2 investigated via transient expression of 35S::CcNAC1: :GFP and 35S::CcNAC2::GFP fusion constructs in Arabidopsis protoplasts, revealed nuclear localization. The transactivation ability of CcNACs was examined in the GAL4 yeast assay system, and showed that only the C-terminal domain of CcNAC1 has the ability to activate reporter genes LacZ and His3. The CcNAC genes accumulated in a tissue-specific manner with expression levels in male flowers of C. colocynthis higher than leaves, hypocotyls or roots. Genome walking was used to isolate the CcNAC1 and CcNAC2-promoter regions. A high number of stress-related sequence motifs were detected, especially in the CcNAC1 promoter. C. colocynthis seedlings were treated with PEG, abscisic acid, salicylic acid (SA), jasmonic acid (JA), H₂O₂, ethylene, gibberellic acid (GA), wounding or salt. High CcNAC1 expression levels were detected following JA application, and wounding, while high CcNAC2 levels followed treatment with GA, JA, SA, and wounding, indicative of differential regulation of these stress responsive TFs in this cucurbit species.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
36
Numer
03
Opis fizyczny
p.621-634,fig.,ref.
Twórcy
autor
  • Department of Horticulture, Auburn University, Auburn, AL, 36849, USA
  • Department of Biological Sciences, Auburn University, Auburn, AL, 36849, USA
autor
  • Department of Biological Sciences, Auburn University, Auburn, AL, 36849, USA
autor
  • Department of Horticulture, Auburn University, Auburn, AL, 36849, USA
Bibliografia
  • Al-Zahrani HS, Al-Amer KH (2006) A comparative study on Citrullus colocynthis plant grown in different altitudinal locations in Saudi Arabia. Am-Eurasian J Sci Res 1:1–7
  • Ashraf M (2010) Inducing drought tolerance in plants: recent advances. Biotech Adv 28:169–183
  • Atkinson NJ, Urwin PE (2012) The interaction of plant biotic and abiotic stresses: from genes to the field. J Exp Bot 63:3523–3544
  • Bari R, Jones JDG (2009) Role of plant hormones in plant defense responses. Plant Mol Biol 69:473–488
  • Barker SS, Wilhelm KS, Thomashow MF (1994) The 5'-region of Arabidopsis thaliana cor15a has cis-acting elements that confer cold-, drought- and ABA-regulated gene expression. Plant Mol Biol 24:710–713
  • Bostock RM (2005) Signal crosstalk and induced resistance: straddling the line between cost and benefit. Annu Rev Phytopathol 43:545–580
  • Burkill HM (1985) The useful plants of West Tropical Africa, vol 2. Royal Botanic Gardens, Kew Dane F, Liu J, Zhang C (2006) Phylogeography of the bitter apple, Citrullus colocynthis. Genet Resour Crop Ev 54:327–336
  • Delessert C, Kazan K, Wilson LW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R (2005) The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis. Plant J 43:745–757
  • Diaz-De Leon F, Klotz KL, Lagrimini M (1993) Nucleotide sequence of the tobacco (Nicotinana tabacum) anionic peroxidase gene. Plant Physiol 101:1117–1118
  • Doares SH, Narvaez-Vasquez J, Conconi A, Ryan CA (1995) Salicylic-acid inhibits synthesis of proteinase-inhibitors in tomato leaves induced by systemin and jasmonic acid. Plant Physiol 108:1741–1746
  • Ernst HA, Olsen AN, Skriver K, Larsen S, Leggio LL (2004) Structure of the conserved domain of ANAC, a member of the NAC family of transcription factors. EMBO Rep 5:297–303
  • Faria DA, Mamani EMC, Pappas GP Jr, Grattapaglia D (2011) Genotyping systems for Eucalyptus based on tetra-, penta-, and hexanucleotide repeat EST microsatellites and their use for individual fingerprinting and assignment tests. Tree Genet Genomes 7:63–77
  • Fujita M, Fujita Y, Maruyama K, Seki M, Hiratsu K, Ohme-Takagi M, Tran LSP, Yamaguchi-Shinozaki K, Shinozaki K (2004) A dehydration-induced NAC protein, RD26, is involved in a novel ABA-dependent stress-signaling pathway. Plant J 39:863–876
  • Fujita M, Fujita Y, Noutushi Y, Takahashi F, Narusaka Y, Yamaguchi-Shinozaki K, Shinozaki K (2006) Crosstalk between abiotic and biotic stress responses: a current view from the points of convergence in the stress signaling networks. Curr Opin Plant Biol 9:436–442
  • Giraudat J, Parcy F, Bertauche N, Gosti F, Leung J, Morris PC, Bouvier-Durand M, Vartanian N (1994) Current advances in abscisic acid action and signaling. Plant Mol Biol 26:1557–1577
  • Guo S et al (2013) The draft genome of watermelon (Citrullus lanatus) and resequencing of 20 diverse accessions. Nat Genet 45:51–58
  • Hao YJ, Song QX, Chen HW, Zou HF, Wei W, Kang XS, Ma B, Zhang WK, Zhang JS, Chen SY (2010) Plant NAC-type transcription factor proteins contain a NARD domain for repression of transcriptional activation. Planta 232:1033–1043
  • He XJ, Mu RL, Cao WH, Zhang ZG, Zhang JS, Chen SY (2005) AtNAC2, a transcription factor downstream of ethylene and auxin signaling pathways, is involved in salt stress response and lateral root development. Plant J 44:903–916
  • Hu R, Qi G, Kong YZ, Kong DJ, Gao Q, Zhou GK (2010) Comprehensive analysis of NAC domain transcription factor gene family in Populus trichocarpa. BMC Plant Biol 10:145–168
  • Hubo T, Asada M, Kowyama Y, Hattori T (1999) ACGT-containing abscisic acid response element (ABRE) and coupling element 3 (CE3) are functionally equivalent. Plant J 19:679–689
  • Jarret RL, Merrick LC, Holms T, Evans J, Aradhya MK (1997) Simple sequence repeats in watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai). Genome 40:433–441
  • Jeffrey C (2008) A review of Cucurbitaceae. Bot J Linn Soc 81:233–247
  • Jensen MK, Rung JH, Gregersen PL, Gjetting T, Fuglsang AT, Hansen M, Joehnk N, Lyngkjaer MF, Collinge DB (2007) The HvNAC6 transcription factor: a positive regulator of penetration resistance in barley and Arabidopsis. Plant Mol Biol 65:137–150
  • Kato H, Mutomura T, Komeda Y, Saito T, Kato A (2010) Overexpression of the NAC transcription factor family gene ANAC036 results in a dwarf phenotype in Arabidopsis thaliana. J Plant Physiol 167:571–577
  • Kazan K, Manners JM (2012) JAZ repressors and the orchestration of phytohormone crosstalk. Trends Plant Sci 17:22–31
  • Kim SG, Lee AK, Yoon HK, Park CM (2008) A membrane-bound NAC transcription factor NTL8 regulates gibberellic acid-mediated salt signaling in Arabidopsis seed germination. Plant J 55:77–88
  • Kleinow T, Himbert S, Krenz B, Jeske H, Koncz C (2009) NAC domain transcription factor ATAF1 interacts with SNF1-related kinases and silencing of its subfamily causes severe developmental defects in Arabidopsis. Plant Sci 177:360–370
  • Kunkel BN, Brooks DM (2002) Cross talk between signaling pathways in pathogen defense. Curr Opin Plant Biol 5:325–331
  • Laudert D, Weiler EW (1998) Allen oxide synthase: a major control point in Arabidopsis thaliana octadecanoid signaling. Plant J 15:675–684
  • Lescot M, Déhais P, Thijs G, Marchal K, Moreau Y, Van de Peer Y, Rouzé P, Rombauts S (2002) PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Res 30:325–327
  • Maleck K, Levine A, Eulgem T, Morgan A, Schmid J, Lawton KA, Dangl JL, Dietrich RA (2000) The transcriptome of Arabidopsis thaliana during systemic acquired resistance. Nat Genet 26:403–410
  • Manjunath S, Sachs MM (2005) Molecular characterization and promoter analysis of the maize cytosolic glyceraldehyde 3-phosphate dehydrogenase gene family and its expression during anoxia. Unpublished AC (NCBI) pp U45858
  • Michel E, Salamini R, Barels E, Dale P, Baga M, Szalay A (1993) Analysis of a desiccation and ABA-responsive promoter isolated from the resurrection plant Craterostigma plantagineum. Plant J 4:29–40
  • Mongkolsiriwatana C, Pongtongkanm P, Peyachoknagul S (2009) In silico promoter analysis of photoperiod-responsive genes identified by DNA microarray in rice (Oryza sativa L.). J Nat Sci 43:164–177
  • Mundy J, Yamaguchi-Shinozaki K, Chua NH (1990) Nuclear proteins bind conserved elements in the abscisic acid-responsive promoter of a rice rab gene. PNAS 87:1406–1410
  • Murphy AM, Carr JP (2002) Salicylic acid has cell-specific effects on tobacco mosaic virus replication and cell-to-cell movement. Plant P 128:552–563
  • Nakashima K, Tran LP, Nguyen DV, Fujita M, Maruyama K, Todaka D, Ito Y, Hayashi N, Shinozaki K, Yamaguchi-Schinozaki K (2007) Functional analysis of a NAC-type transcription factor OsNAC involved in abiotic and biotic stress-responsive gene expression in rice. Plant J 51:617–630
  • Oliveira TM, Cidade LC, Gesteira AS, Coelho Filho MA, Soares Filho WS, Costa MGC (2011) Analysis of the NAC transcription factor gene family in citrus reveals a novel member involved in multiple abiotic stress response. Tree Genet Genomes 7:1123–1134
  • Olsen AN, Ernst HA, Leggio LL, Skriver K (2005) NAC transcription factors: structurally distinct, functionally diverse. Trends Plant Sci 2:79–87
  • Ooka H, Satoh K, Doi K, Nagata T, Otomo Y, Murakami K, Matsubara K, Matsubara K, Osato N, Kawai J, Caminci P, Hayashizaki Y, Suzuki K, Kojima K, Takahara Y, Yamamoto K, Kikuchi S (2003) Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana. DNA Res 10:239–247
  • Peng H, Cheng HY, Chen C, Yu XW, Yang JN, Gao WR, Shi QH, Zhang H, Li JG, Ma H (2009) A NAC transcription factor gene of Chickpea (Cicer arietinum), CarNAC3, is involved in drought stress response and various developmental processes. J Plant Physiol 166:1934–1945
  • Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT–PCR. NAR 29:e45
  • Puranik S, Sahu PP, Srivastava PS, Prasad M (2012) NAC protein: regulation and role in stress tolerance. Trends Plant Sci 17:369–381
  • Quail MA, Smith M, Coupland P, Otto TD, Harris SR, Connor TR, Bertoni A, Swerdlow HP, Gu Y (2012) A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers. BMC Genomics 13:341–354
  • Rashotte AM, Mason MG, Hutchison CE, Ferreira FJ, Schaller GE, Kieber JJ (2006) A subset of Arabidopsis AP2 transcription factors mediates cytokinin responses in concert with a two component pathway. PNAS 103:11081–11085
  • Rouster J, Leah R, Mondy J, Cameron-Millis V (1997) Identification of a methyl jamonate-responsive region in the promoter of a lipoxygenase 1 gene expressed in barley again. Plant J 11:513–523
  • Saibo NJM, Lourenc OT, Oliveira MM (2009) Transcription factors and regulation of photosynthetic and related metabolism under environmental stresses. Ann Bot 103:609–623
  • Salzman RA, Brady JA, Finlayson SA, Buchanan CD, Summer EJ, Sun F, Klein PE, Klein RR, Pratt LH, Cordonnier-Pratt MM, Mullet JE (2005) Transcriptional profiling of sorghum induced by methyl jamonate, salicylic acid, and aminocyclopropane carboxylic acid reveals cooperative regulation and novel gene response. Plant Physiol 138:352–368
  • Schramm F, Ganguli A, Kiehlmann E, Englich G, Walch D, von Koskull-Doring P (2006) The heat stress transcription factor HsfA2 serves as a regulatory amplifier of a subset of genes in the heat stress response in Arabidopsis. Plant Mol Biol 60:759–772
  • Sheen J, Zhou L, Jang JC (1999) Sugars as signaling molecules. Curr Opin Plant Biol 2:410–418
  • Shi J, Zhang L, An H, Wu C, Guo X (2011) GhMPK16, a novel stress-responsive group D MAPL gene from cotton, is involved in disease resistance and drought sensitivity. BMC Mol Biol 12:22–37
  • Si Y, Zhang C, Meng S, Dane F (2009) Gene expression changes in response to drought stress in Citrullus colocynthis. Plant Cell Rep 28:997–1009
  • Si Y, Huang Y, Kang KK, Dane F (2010a) Impact of grafting on NAC gene expression in Citrullus colocynthis and Citrullus lanatus during drought. Cucurbitaceae 2010:290–293
  • Si Y, Dane F, Rashotte A, Kang K, Singh NK (2010b) Cloning and expression analysis of the Ccrboh gene encoding respiratory burst oxidase in Citrullus colocynthis and grafting onto Citrullus lanatus (watermelon). J Exp Bot 61:1635–1642
  • Steppuhn A, Gaquerel E, Baldwin IT (2010) The two α-dox genes of Nicotiana attenuate: overlapping but distinct functions in development and stress responses. BMC Plant Biol 10:171–186
  • Swindell WR (2006) The association among gene expression response to nine abiotic stress treatments in Arabidopsis thaliana. Genetics 174:1811–1824
  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular Evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
  • Tao Y, Xie ZY, Chen WQ, Glazebrook J, Chang HS, Han B, Zhu T, Zou GZ, Katagiri F (2003) Quantitative nature of Arabidopsis responses during compatible and incompatible interactions with the bacterial pathogen Pseudomonas syringae. Plant Cell 15:317–330
  • Tuteja N, Sopory SK (2008) Chemical signaling under abiotic stress environment in plants. Plant Signal Behav 3:5205–5536
  • Vasyukova NI, Chalenko GI, Gerasimova NG, Ozeretskovskaya OL (2011) Wound repair in plant tissues. Appl Biochem Microbiol 47:229–233
  • Walker JC, Howard EA, Dennis ES, Peacock WJ (1987) DNA sequences required for anaerobic expression of the maize Adh1 gene. PNAS 84:6624–6629
  • Wang Z, Dane F (2013) NAC (NAM/ATAF/CUC) transcription factors in different stresses and their signaling pathway. Acta Physiol Plant. doi:10.1007/s11738-012-1195-4
  • Wang L, Li P, Brutnell TP (2010) Exploring plant transcriptomes using ultra high-throughput sequencing. Brief Funct Genomic Proteomic 9:118–128
  • Wang Y, Liu GJ, Yang XF, Wei ZG, Xu ZR (2011) MeJa-inducible expression of the heterologous JAZ2 promoter from Arabidopsis in Populus trichocarpa protoplasts. JPDP 118:69–74
  • Xu ZS, Chen M, Li LC, Ma YZ (2011) Functions and application of the AP2/ERF transcription factor family in crop improvement. J Integr Plant Biol 53:570–585
  • Yamaguchi-Shinozaka K, Mundy J, Chua NH (1989) Four tightly linked rab genes are differentially expressed in rice. Plant Mol Biol 14:29–39
  • Zamir D, Navot N, Rudich J (1984) Enzyme polymorphism in Citrullus lanatus and C. colocynthis in Israel and Sinai. Plant Syst Evol 146:163–170
  • Zheng X, Chen B, Lu G, Han B (2009) Overexpression of a NAC transcription factor enhances rice drought and salt tolerance. Biochem Biophy Res Commun 379:985–989
  • Zohary D, Hopf M (2000) Domestication of plants in the old world, 3rd edn. Clarendon Press, Oxford
Typ dokumentu
Bibliografia
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Identyfikator YADDA
bwmeta1.element.agro-3934dfb3-9697-44f2-bc49-1cd76ad1978f
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