Repositioning of selected genes in neuronal cell nucleus upon seizures

• Autorzy: Walczak A. , Szczepankiewicz A. , Szczepinska T. , Ruszczycki B. , Dabrowski M. , Broszkiewicz M. , Siudek M. , Pyskaty M. , Wilczek E. , Szczerbal I. , Switonski M. , Pawlowski K. , Wilczynski G.
• Języki publikacji: EN

Abstrakty

EN

Even though the molecular mechanisms of gene expression in neurons are quite broadly described in the literature, little is known about the relationship between these processes and the architecture of the neuronal cell nucleus. For example, it is firmly established that waves of gene expression occurring after neuronal stimulation comprise plenty of genes playing important roles in cognitive and epileptic phenomena. However, it has never been examined whether these bursts of transcriptional activity involve any regulation at the level of higher-order nuclear structure. Accordingly, we have performed studies on the structure of neuronal nucleus in epileptic animals. Based on the literature we chose the genes for BDNF and TRKB that play key roles in synaptic plasticity in the brain. We have investigated clustering of these genes in the nucleus by means of fluorescent in situ hybridization. We found that in hippocampal neurons the bdnf-trkb distance decreases 2 hours after seizure induction compared to control. Moreover, using bioinformatical approach we selected 2 linear clusters of the genes that are upregulated after seizures. One of them was selected on the chromosome 1 and the other one on chromosome 20. Those linear gene clusters appeared to associate 4 weeks after seizure compared to control. Our results suggest that upon epileptogenesis there is a reorganization of neuronal nucleus involving clustering of the genes in three-dimensional space. The phenomenon could occur also in other forms of synaptic plasticity, and should be a topic to follow up in the future.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2011
• Tom: 71
• Numer: S
• Opis fizyczny: p.50

Twórcy

autor

Walczak A.

• Department of Neurophysiology, Nencki Institute of Experimental Biology PAS, Warsaw, Poland

autor

Szczepankiewicz A.

• Department of Neurophysiology, Nencki Institute of Experimental Biology PAS, Warsaw, Poland

autor

Szczepinska T.

• Department of Molecular and Neurobiology, Nencki Institute of Experimental Biology PAS, Warsaw, Poland

autor

Ruszczycki B.

• Department of Molecular and Neurobiology, Nencki Institute of Experimental Biology PAS, Warsaw, Poland

autor

Dabrowski M.

• Department of Molecular and Neurobiology, Nencki Institute of Experimental Biology PAS, Warsaw, Poland

autor

Broszkiewicz M.

• Department of Neurophysiology, Nencki Institute of Experimental Biology PAS, Warsaw, Poland

autor

Siudek M.

• Department of Neurophysiology, Nencki Institute of Experimental Biology PAS, Warsaw, Poland

autor

Pyskaty M.

• Department of Neurophysiology, Nencki Institute of Experimental Biology PAS, Warsaw, Poland

autor

Wilczek E.

• Department of Histopathology, Medical University of Warsaw, Warsaw, Poland

autor

Szczerbal I.

• Department of Animal Breeding, Poznan University of Life Sciences, Poznan, Poland

autor

Switonski M.

• Department of Animal Breeding, Poznan University of Life Sciences, Poznan, Poland

autor

Pawlowski K.

• Department of Molecular and Neurobiology, Nencki Institute of Experimental Biology PAS, Warsaw, Poland

autor

Wilczynski G.

• Department of Neurophysiology, Nencki Institute of Experimental Biology PAS, Warsaw, Poland

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