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2011 | 33 | 5 |

Tytuł artykułu

Re-evaluation of the effects of growth regulators on callus induction and shoot regeneration in Agrobacterium-mediated transformation of lettuce

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Lettuce (Lactuca sativa) transformation varies by genotype. Various culture parameters have been studied in order to improve the transformation efficiency of lettuce cultivars. However, no improved transformation procedure for recalcitrant lettuce cultivars has yet been established. Here, we demonstrate the effects of varying concentrations and distinct combinations of growth regulators on recalcitrant lettuce transformation efficiency. More precisely, we assessed differences in the effects of several growth regulator combinations, including N-6(2-isopentenyl)-adenine (2ip), on induction of callus and regeneration of shoots after co-cultivation with Agrobacterium. When two commercial recalcitrant cultivars, Red Romaine and Bibb, were cultured on a medium with 2ip 1 mg l⁻¹, IAA 0.1 mg l⁻¹, and subsequently transferred to a second medium with BA 0.4 mg l⁻¹, NAA 0.05 mg l⁻¹ for selection and shoot regeneration, transformation efficiencies reached 8 and 9%, respectively. Stable integration and transmission of the transgene in T1 generation plants were confirmed by molecular analysis. This procedure represents a simple, efficient, and general means of transforming various lettuce cultivars, including recalcitrant commercial cultivars.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

33

Numer

5

Opis fizyczny

p.1631-1637,fig.,ref.

Twórcy

autor
  • Department of Horticulture, Forestry and Recreation Resources, Kansas State University, Manhattan, KS, 66506, USA
autor
  • Department of Horticulture, Forestry and Recreation Resources, Kansas State University, Manhattan, KS, 66506, USA
autor
  • Department of Horticulture, Forestry and Recreation Resources, Kansas State University, Manhattan, KS, 66506, USA

Bibliografia

  • Curtis IS (2006) Lettuce (Lactuca sativa L.). In: K. Wang (ed) Methods in Molecular Biology, Humana Press Inc., USA, pp 449–458
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  • Curtis IS, He C, Power JB, Mariotti D, De Laat A, Davey MR (1996) The effects of Agrobacterium rhizogenes rolAB genes in lettuce. Plant Sci 115:123–135
  • Curtis IS, Power JB, de Laat AMM, Caboche M, Davey MR (1999) Expression of a chimeric nitrate reductase gene in transgenic lettuce reduces nitrate in leaves. Plant Cell Rep 18:889–896
  • Curtis IS, Nam HG, Sakamoto K (2004) Optimized shoot regeneration system for the commercial Korean radish ‘Jin Ju Dae Pyong’. Plant Cell Tissue Organ Cult 77:81–87
  • Feskanich D, Korrick S, Greenspan S, Rosen H, Colditz G (1999) Moderate alcohol consumption and bone density among postmenopausal women. J Womens Health 8:65–73
  • Ganesan M, Jayabalan N (2006) Influence of cytokinins, auxins and polyamines on in vitro mass multiplication of cotton (Gossypium hirsutum L. cv. SVPR2). Indian J Exp Biol 44:506–513
  • Hoekema A, Hirsch PR, Hooykaas PJJ, Schilperoort RA (1983) A binary plant vector strategy based on separation of vir-region and t-region of the Agrobacterium tumefaciens Ti-plasmid. Nature 303:179–180
  • Hu W, Phillips GC (2001) A combination of overgrowth-control antibiotics improves Agrobacterium tumefaciens-mediated transformation efficiency for cultivated tomato (L. esculentum). In Vitro Cell Dev Biol Plant 37:12–18
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Bibliografia

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