Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2011 | 33 | 5 |
Tytuł artykułu

Re-evaluation of the effects of growth regulators on callus induction and shoot regeneration in Agrobacterium-mediated transformation of lettuce

Treść / Zawartość
Warianty tytułu
Języki publikacji
Lettuce (Lactuca sativa) transformation varies by genotype. Various culture parameters have been studied in order to improve the transformation efficiency of lettuce cultivars. However, no improved transformation procedure for recalcitrant lettuce cultivars has yet been established. Here, we demonstrate the effects of varying concentrations and distinct combinations of growth regulators on recalcitrant lettuce transformation efficiency. More precisely, we assessed differences in the effects of several growth regulator combinations, including N-6(2-isopentenyl)-adenine (2ip), on induction of callus and regeneration of shoots after co-cultivation with Agrobacterium. When two commercial recalcitrant cultivars, Red Romaine and Bibb, were cultured on a medium with 2ip 1 mg l⁻¹, IAA 0.1 mg l⁻¹, and subsequently transferred to a second medium with BA 0.4 mg l⁻¹, NAA 0.05 mg l⁻¹ for selection and shoot regeneration, transformation efficiencies reached 8 and 9%, respectively. Stable integration and transmission of the transgene in T1 generation plants were confirmed by molecular analysis. This procedure represents a simple, efficient, and general means of transforming various lettuce cultivars, including recalcitrant commercial cultivars.
Słowa kluczowe
Opis fizyczny
  • Department of Horticulture, Forestry and Recreation Resources, Kansas State University, Manhattan, KS, 66506, USA
  • Department of Horticulture, Forestry and Recreation Resources, Kansas State University, Manhattan, KS, 66506, USA
  • Department of Horticulture, Forestry and Recreation Resources, Kansas State University, Manhattan, KS, 66506, USA
  • Curtis IS (2006) Lettuce (Lactuca sativa L.). In: K. Wang (ed) Methods in Molecular Biology, Humana Press Inc., USA, pp 449–458
  • Curtis IS, Power JB, Blackhall NW, Delaat AMM, Davey MR (1994) Genotype-independent transformation of lettuce using Agrobacterium tumefaciens. J Exp Bot 45:1441–1449
  • Curtis IS, He C, Power JB, Mariotti D, De Laat A, Davey MR (1996) The effects of Agrobacterium rhizogenes rolAB genes in lettuce. Plant Sci 115:123–135
  • Curtis IS, Power JB, de Laat AMM, Caboche M, Davey MR (1999) Expression of a chimeric nitrate reductase gene in transgenic lettuce reduces nitrate in leaves. Plant Cell Rep 18:889–896
  • Curtis IS, Nam HG, Sakamoto K (2004) Optimized shoot regeneration system for the commercial Korean radish ‘Jin Ju Dae Pyong’. Plant Cell Tissue Organ Cult 77:81–87
  • Feskanich D, Korrick S, Greenspan S, Rosen H, Colditz G (1999) Moderate alcohol consumption and bone density among postmenopausal women. J Womens Health 8:65–73
  • Ganesan M, Jayabalan N (2006) Influence of cytokinins, auxins and polyamines on in vitro mass multiplication of cotton (Gossypium hirsutum L. cv. SVPR2). Indian J Exp Biol 44:506–513
  • Hoekema A, Hirsch PR, Hooykaas PJJ, Schilperoort RA (1983) A binary plant vector strategy based on separation of vir-region and t-region of the Agrobacterium tumefaciens Ti-plasmid. Nature 303:179–180
  • Hu W, Phillips GC (2001) A combination of overgrowth-control antibiotics improves Agrobacterium tumefaciens-mediated transformation efficiency for cultivated tomato (L. esculentum). In Vitro Cell Dev Biol Plant 37:12–18
  • Kanamoto H, yamashita A, Asao H, Okumura S, Takse H, Hattori M, yokota A, Tomizawa K-I (2006) Efficient and stable transformation of Lactuca sativa L. cv. Cisco (lettuce) plastids. Transgenic Res 15:205–217
  • Lelivelt CLC, McCabe MS, Newell CA, de Snoo CB, van Dun KMP, Birch-Machin I, Gray JC, Mills KHG, Nugent JM (2005) Stable plastid transformation in lettuce (Lactuca sativa L.). Plant Mol Biol 58:763–774
  • Lim W, Earle DE (2008) Effect of in vitro and in vivo colchicine treatments on pollen production and fruit recovery on melon plants obtained after pollination with irradiated pollen. Plant Cell Tissue Organ Cult 95:115–124
  • Lim W, Earle DE (2009) Enhanced fruit set from parthenogenetic melon plants via colchicine treatment of nodal explants. Plant Cell Tissue Organ Cult 98:351–356
  • Michelmore R, Marsh E, Seely S, Landry B (1987) Transformation of lettuce (Lactuca sativa) mediated by Agrobacterium tumefaciens. Plant Cell Rep 6:439–442
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497
  • Nicolle C, Carinault N, Gueux L, Jaffrelo L, Rock E, Mazur A, Amouroux P, remesy C (2004) Health effect of vegetable-based diet: lettuce consumption improves cholesterol metabolism and antioxidant status in the rat. Clin Nutr 23:605–614
  • Park SH, Morris JL, Park JE, Hirschi KD, Smith RH (2003) Efficient and genotype-independent Agrobacterium-mediated tomato transformation. J Plant Physiol 160:1253–1257
  • Park SH, Elless MP, Park J, Jenkins A, Lim W, Chambers IV E, Hirschi KD (2009) Sensory analysis of calcium biofortified lettuce. Plant Biotechnol J 7:106–117
  • SAS Institute (2004) SAS/STAT 9.1. User’s guide. SAS Institute Inc, Cary
  • Service/USDA ER (2009) Tables for fresh vegetables.
  • Stachel S, Zambryski P (1986) VirA and virG control the plant induced activation of the T-DNA transfer process of A tumefaciens. Plant Cell Tissue Organ Cult 34:311–314
  • Sun H-J, Uchii S, Watanabe S, Ezura H (2006) A highly efficient transformation protocol for Micro-Tom, a model cultivar for tomato functional genomics. Plant Cell Physiol 47:426–431
  • Torres AC, Cantliffe DJ, Laughner B, Bieniek M, Nagata R, Ashraf M, Ferl RJ (1993) Stable transformation of lettuce cultivar South Bay from cotyledon explants. Plant Cell Tissue Organ Cult 34:279–285
  • Vanjildorj E, Bae TW, Riu KZ, Kim SY, Lee HY (2005) Overexpression of Arabidopsis ABF3 gene enhances tolerance to drought and cold in transgenic lettuce (Lactuca sativa). Plant Cell Tissue Organ Cult 83:41–50. doi:10.1007/s11240-005-3800-3
  • Zhu Y, Ouyang W, Li Y, Chen Z (1996) The effects of 2ip and 2, 4-D on rice calli differentiation. Plant Growth Regul 19:19–24
Rekord w opracowaniu
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.