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2012 | 15 | 4 |

Tytuł artykułu

The value of Fluorescence Polarisation Assay in verification of problematic sera from pigs for brucellosis

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Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The aim of the study was an evaluation of fluorescence polarisation assay (FPA) as a potential tool improving specificity of serological diagnosis of brucellosis in pigs. The evaluation was done by comparing the results of FPA with the results of rose bengal test (RBT), serum agglutination test (SAT), complement fixation test (CFT) and ELISA when false positive sera were tested. One hundred ninety porcine samples, reacting positively in at least one classical serological assay were used. We observed that among 198 sera, 104 were also positive in FPA. The studies confirmed that porcine FPA adds little as far as specificity in comparison to other methods is concerned.

Słowa kluczowe

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-

Rocznik

Tom

15

Numer

4

Opis fizyczny

Twórcy

autor
  • Department of Microbiology of the National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland
autor
  • Department of Microbiology of the National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland
autor
  • Department of Microbiology of the National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland

Bibliografia

  • Garin-Bastuji B, Hummel N, Gerbier G, Cau C, Pouillot R, Da Costa M, Fontaine JJ (1999) Non specific serological reactions in the diagnosis of bovine brucellosis: experimental oral infection of cattle with repeated doses of Yersinia enterocolitica O:9. Vet Microbiol 66: 223-233.
  • Nielsen K, Gall D, Bermudez R, Renteria T, Moreno F, Corral A, Monroy O, Monge F, Smith P, Widdison J, Mardrueno M, Calderon N, Guerrero N, Tinoco R, Osuna J, Kelly W (2002) Field trial of the brucellosis fluorescence polarization assay. J Immunoassay Immunochem 23: 307-316.
  • Paulo PS, Vigliococco AM, Ramondino RF, Marticorena D, Bissi E, Briones G, Gorchs C, Gall D, Nielsen K (2000) Evaluation of Primary Binding Assays for Presumptive Serodiagnosis of Swine Brucellosis in Argentina. Clin Diagn Lab Immunol 7: 828-831.
  • Szulowski K, Iwaniak W, Zlotnicka J, Weiner M, Zareba Z, Czempinska H (2011) International trade – a potential source of brucellosis in pigs. Med Weter 67: 64-66.
  • Szulowski K, Pilaszek J, Truszczyński M (1996) An ELISA – kit for the examination of swine sera for brucellosis. Med Weter 52: 513-515.
  • Weiner M, Iwaniak W, Zlotnicka J, Szulowski K (2010) Diagnosis of bovine brucellosis using traditional serological techniques and fluorescence polarisation assay. Bull Vet Inst Pulawy 54: 485-488.

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Typ dokumentu

Bibliografia

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bwmeta1.element.agro-38f8d46b-24a6-493f-8470-0fc1152f8f1d
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