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2015 | 37 | 11 |
Tytuł artykułu

The effect of moisture content and temperature on spore aging in Osmunda regalis

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
For spores, like seeds, the moisture level and storage temperature seem to be crucial factors responsible for their survival. However, in chlorophyllous spores in particular, the problem is not yet fully understood. The aim of this study was to investigate the effect of moisture content (MC; 6.6 and 4.6 %) and storage temperature (15 and -196 ºC) on spore germination and gametophyte development in Osmunda regalis. The MC (fresh weight basis) in freshly released spores was 7.5 %, and decreased to 6.6 and 4.6 % during 2 weeks of spore storage at 42 and 18 % relative humidity, respectively. Those spores germinated at 99.9 % within 28 h. The spores with 6.6 and 4.6 % MC were used for the storage at 15 and -196 ºC. The deterioration of spores, maintained with 6.6 % MC, proceeds within 1.5 years of storage at 15 ºC, starting with month 8. For the spores with 4.6 % MC serious disturbances in germination and capacity to gametophyte development were postponed for more than 6 months. The time required for spore germination increased with the age of the spores, ranging from 28 h in fresh spores to 10 days in spores stored for more than 11 months. Following 7-year-long cryostorage, spore viability remained at 99.9 %, the time taken for germination remained unaltered and gametophyte development was normal. The viability and vigor of spores were directly correlated with their age, the moisture content and storage temperature.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
37
Numer
11
Opis fizyczny
Article: 229 [11 p.], fig.,ref.
Twórcy
autor
  • Polish Academy of Sciences Botanical Garden - Center for Biological Diversity Conservation in Powsin, Prawdziwka 2, 02-973, Warsaw, Poland
autor
  • Polish Academy of Sciences Botanical Garden - Center for Biological Diversity Conservation in Powsin, Prawdziwka 2, 02-973, Warsaw, Poland
autor
  • Polish Academy of Sciences Botanical Garden - Center for Biological Diversity Conservation in Powsin, Prawdziwka 2, 02-973, Warsaw, Poland
  • Polish Academy of Sciences Botanical Garden - Center for Biological Diversity Conservation in Powsin, Prawdziwka 2, 02-973, Warsaw, Poland
  • Polish Academy of Sciences Botanical Garden - Center for Biological Diversity Conservation in Powsin, Prawdziwka 2, 02-973, Warsaw, Poland
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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