Comparative toxicological effects of orally and intraperitoneally administered aqueous extracts of Abrus precatorius leaf in Mus musculus

• Autorzy: Saganuwan S. , Onyeyili P. A. , Suleiman A. O.

Warianty tytułu

PL

Porównanie toksyczności dożołądkowego oraz dootrzewnowego podawania wodnych ekstraktów z liści modligroszka pospolitego (Abrus precatorious) w badaniach na myszach

• Języki publikacji: EN

Abstrakty

EN

Phytochemical analysis revealed the presence of alkaloids, flavonoids, saponins, tannins, reducing sugar, total glycoside and saponin glycosides in high concentration. Proximate analysis revealed the presence of moisture (6.18%), dry matter (93.90%), crude protein (12.06%), crude fibre (15.33%), nitrogen free extract (34.12%), ash (12.28%) and oil (19.21%). Comparative toxicological assessment of orally and intraperitoneally administered aqueous extracts of Abrus precatorius leaf was carried out in Swiss albino mice. Median lethal doses (LD50) of orally and intraperitoneally administered aqueous extracts of Abrus precatorius leaf were estimated at dose levels of 2558.9 mg/kg and 638 mg/kg body weight, respectively. Intraperitoneal 10 mg/kg body weight of the extract caused increased packed cell volume, neutropenia and decreased aspartate aminotransferase. However, 50 mg/kg oral dose caused increased packed cell volume, neutropenia, decreased alkaline phosphatase and hypochloraemia, whereas oral aqueous dose of 250 mg/kg of body weight caused body weight gain, neutropenia, decreased asparatate aminotransferase and alanineaminotransferase. All the test doses caused lymphocytosis and hypercreatinaemia, hence aqueous extract of Abrus precatorius leaf is toxic at dose levels of 10, 50 and 250 mg/kg body weight.

PL

Analiza fitochemiczna wodnego wyciągu z liści Abrus precatorious wykazała obecność dużych ilości alkaloidów, saponin, tanin, cukrów redukujących, sumy glikozydów oraz glikozydów saponinowych. W dalszych analizach oznaczono: wilgotność (6,18%), suchą masę (93,90%) oraz zawartość białka (12,06%), włókna (15,33%), ekstraktu wolnego od azotu (34,12%), popiołu (12.28%), oleju (19,21%). Badanie toksyczności ekstraktu wodnego z liści Abrus precatorious podawanego dożołądkowo oraz dootrzewnowo przeprowadzono na myszach szczepu Swiss. LD50 dla dożołądkowego oraz dootrzewnowego podawania wodnego wyciągu z liści modligroszku pospolitego wynosiła odpowiednio 2558,9 mg/kg oraz 638 mg/kg masy ciała myszy. Dootrzewnowe podanie ekstraktu w ilości 10 mg/kg masy ciała myszy powodowało wzrost łącznej objętości elementów morfotycznych krwi (PCV), neutropenię oraz spadek poziomu aminotransferazy asparaginianowej. W przypadku dożołądkowego podania wyciągu w ilości 50 mg/kg m.c. myszy odnotowano spadek łącznej objętości elementów morfotycznych krwi (PCV), neutropenię, wzrost poziomu fosfatazy alkalicznej oraz hipochloremię. Natomiast dożołądkowa dawka wodnego wyciągu w ilości 250 mg/kg m.c. myszy spowodowała wzrost masy ciała, neutropenię, spadek poziomu aminotrasferazy asparaginianowej oraz alaninoaminotransferazy. Wszystkie badane dawki powodowały limfocytozę oraz hiperkreatynemię i z tego powodu należy uznać, że wodny wyciąg z liści Abrus precatorious jest toksyczny w dawkach 10, 50 oraz 250 mg/kg masy ciała myszy.

Słowa kluczowe

EN

toxicological effect; oral administration; intraperitoneal administration; water extract; Abrus precatorius; leaf; plant extract; Mus musculus

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2011
• Tom: 57
• Numer: 3
• Opis fizyczny: p.32-44,ref.

Twórcy

autor

Saganuwan S.

• Department of Veterinary Physiology, Pharmacology and Biochemistry, College of veterinary Medicine, University of Agriculture, P.M.B.2373 Makurdi, Benue State, Nigeria

autor

Onyeyili P. A.

autor

Suleiman A. O.

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