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2015 | 37 | 01 |
Tytuł artykułu

Freezing tolerance in Norway spruce, the potential role of pathogenesis-related proteins

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Cold-tolerant plants may endure subzero temperatures partially by inhibiting the development of ice crystals in the intercellular spaces and the xylem through the accumulation of antifreeze proteins (AFP) and the extra production of carbohydrates. Certain proteins associated with pathogen resistance in plants have the ability to bind and alter the growth of ice crystals. In this study, the accumulation of pathogenesis-related (PR) proteins and the development of freezing tolerance in seedlings of two latitudinal distinct Norway spruce (Picea abies L. Karst.) ecotypes were investigated. Despite freezing tolerance difference, timing of growth cessation and bud set variations, our results showed that there is no significant difference in the concentration of soluble carbohydrates between the two ecotypes. Immunoblots showed the presence of several β-1,3-glucanase and thaumatin PR proteins in the apoplastic fluid and the enzymatic assay showed an extra accumulation of several isoforms of PR chitinases in cold-treated Norway spruce needles. In addition to PR proteins, a presence of de novo protein in cold-treated needles was noticed. In contrary to mature plants, total proteins isolated from freezing-tolerant Norway spruce seedling did not show antifreeze activity. Our results suggest that the activity of the PR proteins and the accumulation of soluble carbohydrates that increased during cold acclimation may have an indirect impact on the freezing tolerance in Norway spruce, however, deciphering the direct mechanism behind freezing tolerance in Norway spruce seedlings growing under controlled environmental conditions require further investigation.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
37
Numer
01
Opis fizyczny
Article 1717 [9 p.], fig.,ref.
Twórcy
autor
  • Norwegian Forest and Landscape Institute, 1431 As, Norway
autor
  • Faculty of Environmental Science and Technology, Norwegian University of Life Science, 1432 As, Norway
autor
  • Sensilect Consulting AB, Spannmalsvagen 42, Dalby, Sweden
autor
  • Department of Biology, College of Science, Sultan Qaboos University, Muscat, Oman
Bibliografia
  • Antikainen M, Griffith M (1997) Antifreeze protein accumulation in freezing-tolerant cereals. Physiol Plant 99:423–432. doi:10.1111/j.1399-3054.1997.tb00556.x
  • Antikainen M, Griffith M, Zhang J, Hon WC, Yang DSC, Pihakaski-Maunsbach K (1996) Immunolocalization of antifreeze proteinsin winter rye leaves crowns, and roots by tissue printing. Plant Physiol 110:845–857
  • Ashworth EN (1996) Responses of bark and wood cells to freezing. Adv Low Temp Biol 3:65–106
  • Ashworth E, Stirm V, Volenec J (1993) Seasonal variations in soluble sugars and starch within woody stems of Cornus sericea L. Tree Physiol 13:379–388
  • Baumann C (1996) Physiological, morphological and molecular biological studies of dormancy development in Norway spruce and scots pine. Doctor Scientiarum theses, Agricultural University of Norway
  • Cabello JV, Arce AL, Chan RL (2012) The homologous HD-Zip I transcription factors HaHB1 and AtHB13 confer cold tolerance via the induction of pathogenesis-related and glucanase proteins. Plant J 69:141–153. doi:10.1111/j.1365-313X.2011.04778.x
  • Dalen LS, Johnsen O (2004) CO(2) enrichment, nitrogen fertilization and development of freezing tolerance in Norway spruce. Trees- Struct Funct 18:10–18. doi:10.1007/s00468-003-0278-7
  • Dalen LS, Johnsen O, Ogner G (2001) CO(2) enrichment and development of freezing tolerance in Norway spruce. Physiol Plant 113:533–540. doi:10.1034/j.1399-3054.2001.1130412.x
  • DeVries AL (1970) Cold resistance in fishes in relation to protective glycoproteins Cryobiology 6:585 http://dx.doi.org/10.1016/S0011-2240(70)80056-6
  • Doxey AC, Yaish MW, Griffith M, McConkey BJ (2006) Ordered surface carbons distinguish antifreeze proteins and their icebinding regions. Nat Biotechnol 24:852–855. doi:10.1038/nbt1224
  • Duman J, Xu L, Neven L, Tursman D, Wu D (1991) Hemolymph proteins involved in insect subzero-temperature tolerance: ice nucleators and antifreeze proteins. In: Lee R Jr, Denlinger D (eds) Insects at low temperature. Springer, US, p 94–127. doi:10.1007/978-1-4757-0190-6_5
  • Glerum C (1985) Frost hardiness of coniferous seedlings: principles and applications. In: Duryea ML (ed) Corvallis, Oregon, Oct 16-18, 1984, 1985. Forest Research Laboratory, Oregon State University, p 107–123. (citeulike-article-id:7215338)
  • Griffith M, Antikainen M (1996) Extracellular ice formation in freezing-tolerant plants. Adv Low Temp Biol 3:107–139
  • Griffith M, Yaish MW (2004) Antifreeze proteins in overwintering plants: a tale of two activities. Trends Plant Sci 9:399–405. doi:10.1016/j.tplants.2004.06.007
  • Griffith M, Ala P, Yang DS, Hon WC, Moffatt BA (1992) Antifreeze protein produced endogenously in winter rye leaves. Plant Physiol 100:593–596
  • Heide O (1974) Growth and dormancy in Norway spruce ecotypes. Physiol Plant 31:131–139
  • Hiilovaara-Teijo M, Hannukkala A, Griffith M, Yu XM, Pihakaski-Maunsbach K (1999) Snow-mold-induced apoplastic proteins in winter rye leaves lack antifreeze activity. Plant Physiol 121:665–674
  • Hon WC, Griffith M, Chong P, Yang D (1994) Extraction and isolation of antifreeze proteins from winter rye (Secale cereale L.) leaves. Plant Physiol 104:971–980
  • Hon WC, Griffith M, Mlynarz A, Kwok YC, Yang DS (1995) Antifreeze proteins in winter rye are similar to pathogenesisrelated proteins. Plant Physiol 109:879–889
  • Institute S (1987) SAS/STAT guide for personal computers: version edition. SAS Institute
  • Jarzabek M, Pukacki PM, Nuc K (2009) Cold-regulated proteins with potent antifreeze and cryoprotective activities in spruces (Picea spp.). Cryobiology 58:268–274
  • Johnsen Ø (1989) Freeze-testing young Picea abies plants: a methodological study. Scand J For Res 4:351–367
  • Johnsen O, Skroppa T, Haug G, Apeland I, Ostreng G (1995) Sexual reproduction in a greenhouse and reduced autumn frost hardiness of Picea abies progenies. Tree Physiol 15:551–555
  • Kärenlampi SO, Airaksinen K, Miettinen AT, Kokko HI, Holopainen JK, Kärenlampi LV, Karjalainen RO (1994) Pathogenesisrelated proteins in ozone-exposed Norway spruce [Picea abies (Karst) L.]. New Phytol 126:81–89
  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
  • Levitt J (1980) Responses of plants to environmental stresses. Water, radiation, salt, and other stresses, vol II, 2nd edn. Academic Press, New York
  • Marentes E, Griffith M, Mlynarz A, Brush RA (1993) Proteins accumulate in the apoplast of winter rye leaves during cold acclimation. Physiol Plant 87:499–507
  • Meyer K, Keil M, Naldrett MJ (1999) A leucine-rich repeat protein of carrot that exhibits antifreeze activity. FEBS Lett 447:171–178
  • Olien CR (1965) Interference of cereal polymers and related compounds with freezing. Cryobiology 2:47–54
  • Pan SQ, Ye XS, Kuc J (1991) A technique for detection of chitinase, β-1,3-glucanase, and protein patterns after a single separation using polyacrylamide gel electrophoresis or isoelectrofocusing. Phytopathology 81:970–974
  • Sharma P (1995) Induced defence responses in spruce (Picea abies (L.) Karst.) roots during infection with a pathogenic fungus, Pythium dimorphum. Agricultural University of Norway
  • Sharma P, Børja D, Stougaard P, Lönneborg A (1993) PR-proteins accumulating in spruce roots infected with a pathogenic Pythium sp. isolate include chitinases, chitosanases and b-1,3-glucanases. Physiol Mol Plant Pathol 43:57–67
  • Steponkus PL (1984) Role of the plasma membrane in freezing injury and cold acclimation. Annu Rev Plant Physiol 35:543–584
  • Tronsmo AM (1984) Predisposing effects of low temperature on resistance to winter stress factors in grasses. Acta Agric Scandinavica 34:210–220
  • Tronsmo A, Gregersen P, Hjeljord L, Sandal T, Bryngelsson T, Collinge D (1993) Cold-induced disease resistance. In: Mechanisms of plant defense responses. Springer, p 369–369
  • Trudel J, Asselin A (1989) Detection of chitinase activity after polyacrylamide gel electrophoresis. Anal Biochem 178:362–366
  • Worrall D et al (1998) A carrot leucine-rich-repeat protein that inhibits ice recrystallization. Science 282:115–117
  • Yaish MW, Doxey AC, McConkey BJ, Moffatt BA, Griffith M (2006) Cold-active winter rye glucanases with ice-binding capacity. Plant Physiol 141:1459–1472. doi:10.1104/pp.106.081935
Typ dokumentu
Bibliografia
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