Cerebellum development in the grey short-tailed opossum

• Autorzy: Tepper B. , Bartkowska K. , Turlejski K. , Djavadian R.
• Języki publikacji: EN

Abstrakty

EN

INTRODUCTION: The grey short-tailed opossum, Monodelphis domestica is a laboratory animal useful in developmental research due to its slow and mostly postnatal growth. Little is known about marsupials brain development. AIM(S): The aim of the study was to investigate cerebellum development in the Monodelphis opossum. METHOD(S): We performed Nissl staining on the brain sectionsfrom opossumsin different age to see the overall pattern of cerebellum development. To find out when progenitor cells are present in this structure, we performed bromodeoxyuridine (BrdU) intraperitoneal injections in different time points. Phenotype of proliferating cells wasidentified by double immunofluorescence staining for a neuronal marker, NeuN or an astrocyte marker, GFAP and BrdU. RESULTS: We found that in the newborn Monodelphis opossum cerebellum is not yet morphologically formed. Immunostainig of BrdU-positive cells showed that Purkinje cells were generated between postnatal day (P) 1–5, whereas the highest rate of granule cells generation occurred between P11–P40. Double immunostaining revealed that the majority of BrdU-positive cells in the opossum cerebellum generated from P11 to P50 was neurons, as they showed colocalization with NeuN immunoreactivity. CONCLUSIONS: Our study shows that cerebellum development in the opossum is longer and more extended in time than in rodents. FINANCIAL SUPPORT: The work was supported by the National Science Center grant 2015/17/B/NZ4/02410.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2017
• Tom: 77
• Numer: Suppl.1
• Opis fizyczny: p.88

Twórcy

autor

Tepper B.

• Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Bartkowska K.

• Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Turlejski K.

• Cardinal Stefan Wyszynski University in Warsaw, Warsaw, Poland

autor

Djavadian R.

• Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland