Dynamic characteristics of sugar accumulation and related enzyme activities in sweet and non-sweet watermelon fruits

• Autorzy: Liu J. , Guo S. , He H. , Zhang H. , Gong G. , Ren Y. , Xu Y.
• Języki publikacji: EN

Abstrakty

EN

Sugar content largely determines watermelon fruit quality. We compared changes in sugar accumulation and activities of carbohydrate enzymes in the flesh (central portion) and mesocarp of elite sweet watermelon line 97103 (Citrullus lanatus subsp. vulgaris) and exotic nonsweet line PI296341-FR (C. lanatus subsp. lanatus) to elucidate the physiological and biochemical mechanisms of sugar accumulation in watermelon fruit. The major translocated sugars, raffinose and stachyose, were more unloaded into sweet watermelon fruit than non-sweet fruit. During the fruit development, acid a-galactosidase activity was much higher in flesh of 97103 than in mesocarp of 97103, in flesh and mesocarp of PI296341-FR fruit. Insoluble acid invertase activity was higher in 97103 flesh than in 97103 mesocarp, PI296341-FR flesh or mesocarp from 18 days after pollination (DAP) to 34 DAP. Changes in soluble acid invertase activity in 97103 flesh were similar to those in PI296341-FR flesh and mesocarp from 18 DAP to full ripening. Sucrose synthase and sucrose phosphate synthase activities in 97103 flesh were significantly higher than those in 97103 mesocarp and PI296341- FR fruits from 18 to 34 DAP. Only insoluble acid invertase and sucrose phosphate synthase activities were significantly positively correlated with sucrose content in 97103 flesh. Therefore, phloem loading, distribution and metabolism of major translocated sugars, which are controlled by key sugar metabolism enzymes, determine fruit sugar accumulation in sweet and non-sweet watermelon and reflect the distribution diversity of translocated sugars between subspecies.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 11
• Opis fizyczny: p.3213-3222,fig.,ref.

Twórcy

autor

Liu J.

• Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
• Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

autor

Guo S.

• Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
• Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

autor

He H.

• Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
• Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

autor

Zhang H.

• Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
• Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

autor

Gong G.

• Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
• Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

autor

Ren Y.

• Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
• Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

autor

Xu Y.

• Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
• Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

Bibliografia

• Bethke PC, Sabba R, Bussan AJ (2009) Tuber water and pressure potentials decrease and sucrose contents increase in response to moderate drought and heat stress. Am J Potato Res 86:519–532
• Brown AC, Summers WL (1985) Carbohydrate accumulation and color development in watermelon. J Am Soc Hortic Sci 110:683–686
• Burger Y, Schaffer AA (2007) The contribution of sucrose metabolism enzymes to sucrose accumulation in Cucumis melo. J Am Soc Hortic Sci 132:704–712
• Dai N, Cohen S, Portnoy V et al (2011) Metabolism of soluble sugars in developing melon fruit: a global transcriptional view of the metabolic transition to sucrose accumulation. Plant Mol Biol 76:1–18
• Dali N, Michaud D, Yelle S (1992) Evidence for the involvement of sucrose phosphate synthase in the pathway of sugar accumulation in sucrose accumulating tomato fruits. Plant Physiol 99:434–443
• Dane F, Liu JR, Zhan CK (2007) Phylogeography of the bitter apple, Citrullus colocynthis. Genet Resou Crop Evol 54:327–336
• Elmstrom GW, Davis PL (1981) Sugars in developing and mature fruits of several watermelon cultivars. J Am Soc Hortic Sci 106:330–333
• Gao Z, Schaffer AA (1999) A novel alkaline alpha-galactosidase from melon fruit with a substrate preference for raffinose. Plant Physiol 119:979–988
• Gaudreault PR, Webb JA (1986) Alkaline a-galactosidase activity and galactose metabolism in the family cucurbitaceae. Plant Sci 45:71–75
• Godt DE, Roitsch T (1997) Regulation and tissue-specific distribution of mRNAs for three extracellular invertase isoenzymes of tomato suggests an important function in establishing and maintaining sink metabolism. Plant Physiol 115:273–282
• Hubbard NL, Huber SC, Pharr DM (1989) Sucrose phosphate synthase and acid invertase as determinants of sucrose concentration in developing muskmelon (Cucumis melo L.) fruits. Plant Physiol 91:1527–1534
• Hubbard NL, Pharr DM, Huber SC (1991) Sucrose phosphate synthase and other sucrose metabolizing enzymes in fruits of various species. Physiol Plant 82:191–196
• Huber SC, Huber LH (1996) Role and regulation of sucrose phosphate synthase in higher plants. Annu Rev Plant Physiol Plant Mol Biol 47:431–444
• Islam SM, Matsui T, Yoshida Y (1996) Carbohydrate content and the activities of sucrose synthase, sucrose phosphate synthase and acid invertase in different tomato cultivars during fruit development. Sci Hortic 65:125–136
• Iwatsubo T, Nakagawa H, Ogura N, Hirabayashi T, Sato T (1992) Acid invertase of melon fruit: immunochemical detection of acid invertases. Plant Cell Physiol 33:1127–1133
• Jin Y, Ni DA, Ruan YL (2009) Posttranslational elevation of cell wall invertase activity by silencing its inhibitor in tomato delays leaf senescence and increases seed weight and fruit hexose level. Plant Cell 21:2072–2089
• Kano Y (1991) Changes of sugar kind and its content in the fruit of watermelon during its development and after harvest. Environ Control Biol 29:159–166
• Karuppiah N, Vadlamudi B, Kaufman PB (1989) Purification and characterization of soluble (cytosolic) and bound (cell wall) isoforms of invertases in barley (Hordeum vulgare) elongating stem tissue. Plant Physiol 91:993–998
• Keller F, Pharr DM (1996) Metabolism of carbohydrates in sink and sources: galactosyl-sucrose oligosaccharide. In: Zamski E, Schaffer AA (eds) Photoassimilate distribution in plants and crops. Marcel Dekker, New York, pp 157–183
• Kleczkowski LA, Kunz S, Wilczynska M (2010) Mechanisms of UDP-Glucose synthesis in plants. Crit Rev Plant Sci 29:191–203
• Leigh R, Rees T, Fuller WA, Banfield J (1979) The location of acid invertase activity and sucrose in the vacuoles of storage roots of beet root (Beta vulgaris). Biochem J 178:539–547
• Lester GE, Arias LS, Lim MG (2001) Muskmelon fruit soluble acid invertase and sucrose phosphate synthase activity and polypeptide profiles during growth and maturation. J Am Soc Hortic Sci 126:33–36
• Li M, Feng F, Cheng L (2012) Expression Patterns of genes involved in sugar metabolism and accumulation during apple fruit development. PLoS ONE 7(3):e33055. doi:10.1371/journal.pone.0033055
• Lowell CA, Tomlinson PT, Koch KE (1989) Sucrose-metabolizing enzymes in transport tissues and adjacent sink structures in developing citrus fruit. Plant Physiol 90:1394–1402
• McCollum TG, Huber DJ, Cantliffe DJ (1988) Soluble sugar accumulation and activity of related enzymes during muskmelon fruit development. J Am Soc Hortic Sci 113:399–403
• Miron D, Schaffer AA (1991) Sucrose phosphate synthase, sucrose synthase, and invertase activities in developing fruit of Lycopersicon esculentum Mill. and the sucrose accumulating Lycopersicon hirsutum Humb. and Bonpl. Plant Physiol 95:623–627
• Pharr DM, Sox HN (1984) Changes in carbohydrate and enzyme levels during the sink to source transition of leaves of Cucumis sativus L., a stachyose translocator. Plant Sci Lett 35:187–193
• Qazia HA, Paranjpeb S, Bhargavaa S (2012) Stem sugar accumulation in sweet sorghum -Activity and expression of sucrose metabolizing enzymes and sucrose transporters. J Plant Physiol 169:605–613
• Ranwala AP, Iwanami SS, Masuda H (1991) Acid and neutral invertases in the mesocarp of developing muskmelon (Cucumis melo L. cv Prince) fruit. Plant Physiol 96:881–886
• Roitsch T, Gonzalez MV (2004) Function and regulation of plant invertases: sweet sensations. Trends Plant Sci 9:606–613
• Schaffer AA, Aloni B, Fogelman E (1987) Sucrose metabolism and accumulation in developing fruit of Cucumis. Phytochemistry 26:1883–1887
• Slewinski TL (2011) Diverse functional roles of monosaccharide transporters and their homologs in vascular plants: a physiological perspective. Mol Plant pp 1–22
• Stepansky A, Kovalski I, Schaffer AA, Perl-Treves R (1999) Variation in sugar levels and invertase activity in mature fruit representing a broad spectrum of Cucumis melo genotypes. Genet Resour Crop Evol 46:53–62
• Strand A, Zrenner R, Trevanion S, Stiff M, Gustafasson P, Gardeström P (2000) Decreased expression of two key enzymes in the sucrose biosynthesis pathway, cytosolic fructose-1,6- bisphosphatase and sucrose phosphate synthase, has remarkably different consequences for photosynthetic carbon metabolism in transgenic Arabidopsis thaliana. Plant J 23(6):759–770
• Sturm A (1999) Invertases. Primary structures, functions, and roles in plant development and sucrose partitioning. Plant Physiol 121:1–8
• Sun J, Loboda T, Sung SJS, Black CC (1992) Sucrose synthase in wild tomato, Lycopersicon chemielewskii, and tomato fruit sink strength. Plant Physiol 98:1163–1169
• Tang GQ, Luscher M, Sturm A (1999) Antisense repression of vacuolar and cell wall invertase in transgenic carrot alters early plant development and sucrose partitioning. Plant Cell 11:177–189
• Vargas WA, Salerno GL (2010) The Cinderella story of sucrose hydrolysis: alkaline/neutral invertases from cyanobacteria to unforeseen roles in plant cytosol and organelles. Plant Sci 178:1–8
• Wingenter K, Schulz A, Wormit A et al (2010) Increased activity of the vacuolar monosaccharide transporter TMT1 alters cellular sugar partitioning, sugar signaling, and seed yield in Arabidopsis. Plant Physiol 154:665–677
• Yativ M, Harary I, Wolf S (2010) Sucrose accumulation in watermelon fruits: genetic variation and biochemical analysis. J Plant Physiol 167:589–596
• Yelle S, Chetelat RT, Dorais M, DeVerna JW, Bennett AB (1991) Sink metabolism in tomato fruit. IV. Genetic and biochemical analysis of sucrose accumulation. Plant Physiol 95:1026–1035
• Zhang B, Tolstikov V, Turnbull C, Hicks LM, Fiehn O (2010) Divergent metabolome and proteome suggest functional independence of dual phloem transport systems in cucurbits. Proc Natl Acad Sci USA 107:13532–13537
• Zhang H, Gong G, Guo S, Ren Y, Xu Y, Ling KS (2011) Screening the USDA watermelon germplasm collection for drought tolerance at the seedling stage. HortScience 46:1245–1248
• Zhu YJ, Komor E, Moore PH (1997) Sucrose accumulation in the sugarcane stem is regulated by the difference between the activities of soluble acid invertase and sucrose phosphate synthase. Plant Physiol 115:609–616

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