A homolog of Class IV HD-Zip transcription factors, EsHdzip1, confers drought resistance in tobacco via enhanced the capacity of water conserving and absorbing

• Autorzy: Zhou C. , Zhu L. , Ma Z. , Wang J.
• Języki publikacji: EN

Abstrakty

EN

Previous studies have indicated that homeodomain-leucine zipper (HD-Zip) transcription factors play important roles during abiotic stress, but there is no information on the functions of HD-Zip genes in a new model plant Eutrema salsugineum for studying plant abiotic stress tolerance. Here, EsHdzip1 (GenBank No. XM_006390503) belonging to the Class IV subgroup of HD-Zip transcription factor family was isolated from E. salsugineum and characterized for its physiological rolesunder drought stress conditions. Transgenic tobacco plants overexpressing EsHdzip1 exhibited increased drought resistance with promoted root growth and reduction of water loss. Furthermore, these transgenic plants had lower ion leakage (IL), malondialdehyde (MDA), and reactive oxygen species (ROS) accumulation, but higher content of osmotic solutes (proline and total soluble sugars) and activities of antioxidant enzymes including superoxide dismutase (SOD) and ascorbate peroxidase (APX) relative to wild-type (WT) plants when subjected to drought stress treatments. The content of abscisic acid (ABA) was also observed to be remarkably higher in the transgenic lines than WT plants under drought stress conditions. In addition, the expression levels of three important stress-related genes (NtP5CS, NtERD10C, and NtLEA5) involved in the osmotic adjustment and water maintenance were significantly higher than WT plants under drought stress conditions. Therefore, we have revealed important roles of the EsHdzip1 gene in response to drought stress, suggesting that this gene has a great potential for improving plant drought tolerance by engineering manipulation.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 07
• Opis fizyczny: fig.,ref.

Twórcy

autor

Zhou C.

• Institute for Applied Microbiology, Anhui Science and Technology University, 233100, Bengbu, China
• Key Laboratory of Bio-organic Fertilizer Creation, Ministry of Agriculture, 233100, Bengbu, China

autor

Zhu L.

• School of Life Science and Technology, Tongji University, 200092, Shanghai, China

autor

Ma Z.

• Institute for Applied Microbiology, Anhui Science and Technology University, 233100, Bengbu, China
• Key Laboratory of Bio-organic Fertilizer Creation, Ministry of Agriculture, 233100, Bengbu, China

autor

Wang J.

• Institute for Applied Microbiology, Anhui Science and Technology University, 233100, Bengbu, China
• Key Laboratory of Bio-organic Fertilizer Creation, Ministry of Agriculture, 233100, Bengbu, China

Bibliografia

• Asada K (1999) The water–water cycle in chloroplasts: scavenging of active oxygens and dissipation of excess photons. Annu Rev Plant Physiol Plant Mol Biol 50:601–639
• Assmann SM (2003) OPEN STOMATA1 opens the door to ABA signaling in Arabidopsis guard cells. Trends Plant Sci 8:151–153
• Bates L, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207
• Belamkar V, Weeks NT, Bharti AK, Farmer AD, Graham MA, Cannon SB (2014) Comprehensive characterization and RNASeq profiling of the HD-Zip transcription factor family in soybean (Glycine max) during dehydration and salt stress. BMC Genom 15:950
• Bucci SJ, Scholz FG, Goldstein G, Meinzer FC, Arce ME (2009) Soil water availability and rooting depth as determinants of hydraulic architecture of Patagonian woody species. Oecologia 160:631–641
• Cabello JV, Chan RL (2012) The homologous homeodomain-leucine zipper transcription factors HaHB1 and AtHB13 confer tolerance to drought and salinity stresses via the induction of proteins that stabilize membranes. Plant Biotechnol J 10:815–825
• Chen M, Zhao Y, Zhuo C, Lu S, Guo Z (2014) Overexpression of a NF-YC transcription factor from bermudagrass confers tolerance to drought and salinity in transgenic rice. Plant Biotechnol J. doi:10.1111/pbi.12270
• Finkelstein RR, Gamplal SS, Rock CD (2002) Abscisic acid signaling in seeds and seedlings. Plant Cell 14:S15–S45
• Gapper C, Dolan L (2006) Control of plant development by reactive oxygen species. Plant Physiol 141:341–345
• Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930
• Harb A, Krishnan A, Ambavaram MM, Pereira A (2010) Molecular and physiological analysis of drought stress in Arabidopsis reveals early responses leading to acclimation in plant growth. Plant Physiol 154:1254–1271
• Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
• Hu H, Dai M, Yao J, Xiao B, Li X, Zhang Q, Xiong L (2006) Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice. Proc Natl Acad Sci USA 103:12987–12992
• Inan G, Zhang Q, Li P, Wang Z, Cao Z, Zhang H, Zhang C, Quist TM, Goodwin SM, Zhu J, Shi H, Damsz B, Charbaji T, Gong Q, Ma S,Fredricksen M, Galbraith DW, Jenks MA, Rhodes D,
• Hasegawa PM, Bohnert HJ, Joly RJ, Bressan RA, Zhu JK (2004) Salt cress. A halophyte and cryophyte Arabidopsis relative model system and its applicability to molecular genetic analyses of growth and development of extremophiles. Plant Physiol 135:1718-1737
• Ingram J, Bartels D (1996) The molecular basis of dehydration tolerance in plants. Annu Rev Plant Physiol Plant Mol Biol 47:377–403
• Jiang M, Zhang J (2001) Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings. Plant Cell Physiol 42:1265–1273
• Kim J, Malladi A, van Iersel MW (2002) Physiological and molecular responses to drought in Petunia: the importance of stress severity. J Exp Bot 63:6335–6345
• Lee LY, Kononov ME, Bassuner B, Frame BR, Wang K, Gelvin SB (2007) Novel plant transformation vectors containing the super promoter. Plant Physiol 145:1294–1300
• Lin RC, Park HJ, Wang HY (2008) Role of Arabidopsis RAP2.4 in regulating light- and ethylene-mediated developmental processes and drought stress tolerance. Mol Plant 1:42–57
• Liu F, Pang SJ (2010) Stress tolerance and antioxidant enzymatic activities in the metabolism of the reactive oxygen species in two intertidal red algae Grateloupia turuturu and Palmaria palmate. J Exp Mar Bio Eco 328:82–87
• Liu H, Li X, Xiao J, Wang S (2012) A convenient method for simultaneous quantification of multiple phytohormones and metabolites: application in study of rice-bacterium interaction. Plant Methods 8:2
• Liu G, Li X, Jin S, Liu X, Zhu L, Nie Y, Zhang X (2014) Overexpression of rice NAC gene SNAC1 improves drought and salt tolerance by enhancing root development and reducing transpiration rate in transgenic cotton. PLoS One 9:e86895
• Lu Y, Li Y, Zhang J, Xiao Y, Yue Y, Duan L, Zhang M, Li Z (2013) Overexpression of Arabidopsis molybdenum cofactor sulfurase gene confers drought tolerance in maize (Zea mays L.). PLoS One 8:e52126
• Magnani E, Barton MK (2011) A per-ARNT-sim-like sensor domain uniquely regulates the activity of the homeodomainleucine zipper transcription factor REVOLUTA in Arabidopsis. Plant Cell 23:567–582
• McKersie BD, Bowley SR, Harjanto E, Leprince O (1996) Water deficit tolerance and field performance of transgenic alfafa overexpressing superoxide dismutase. Plant Physiol 111:1177–1181
• Miyagawa Y, Tamoi M, Shigeoka S (2000) Evaluation of the defense system in chloroplasts tophotooxidative caused by paraquat using transgenic tobacco plants expressing catalase from Escherichia coli. Plant Cell Physiol 41:311–320
• Mizoi J, Shinozaki K, Yamaguchi-Shinozaki K (2012) AP2/ERF family transcription factors in plant abiotic stress responses. Biochim Biophys Acta 1819:86–96
• Mukherjee K, Burglin TR (2006) MEKHLA, a novel domain with similarity to PAS domains, is fused to plant homeodomainleucine zipper III proteins. Plant Physiol 140:1142–1150
• Mukherjee SP, Choudhuri MA (1983) Implications of water stress induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiol Plant 58:166–170
• Nakashima K, Takasaki H, Mizoi J, Shinozaki K, Yamaguchi-Shinozaki K (2012) NAC transcription factors in plant abiotic stress responses. Biochim Biophys Acta 1819:97–103
• Perl A, Perl-Treves R, Galili S, Aviv D, Shalgi E, Malkin S, Galun E (1993) Enhanced oxidative-stress defense in transgenic potato expressing Cu, Zn superoxide dismutases. Theor Appl Genet 85:568–576
• Puppo A, Groten K, Bastian F, Carzaniga R, Soussi M, Lucas MM, de Felipe MR, Harrison J, Vanacker H, Foyer CH (2004) Legume nodule senescence: roles for redox and hormone signaling in the orchestration of the natural aging process. New Phytol 165:683–701
• Schachtman DP, Goodger JQ (2008) Chemical root to shoot signaling under drought. Trends Plant Sci 13:281–287
• Schachtman DP, Shin R (2007) Nutrient sensing and signaling: NPKS. Annu Rev Plant Biol 58:47–69
• Schena M, Davis RW (1992) HD-Zip proteins: members of an Arabidopsis homeodomain protein superfamily. Proc Natl Acad Sci USA 89:3894–3898
• Shimazaki K, Doi M, Assmann SM, Kinoshita T (2007) Light regulation of stomatal movement. Annu Rev Plant Biol 58:219–247
• Sun YJ, Zhu L, Guo JS, Zhou C (2014) Heterologous expression of EsABA1 enhances salt tolerance with increased accumulation of endogenous ABA in transgenic tobacco. Turk J Bot 38:1067–1079
• Wang W, Vinocur B, Altman A (2003) Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta 218:1–14
• Watanabe S, Kojima K, Ide Y, Sasaki S (2000) Effects of saline and osmotic stress on proline and sugar accumulation in Populus euphratica in vitro. Plant Cell Tiss Organ Cult 63:199–206
• Wilkinson S, Davies WJ (2002) ABA-based chemical signalling: the co-ordination of responses to stress in plants. Plant Cell Environ 25:195–210
• Xian L, Sun P, Hu S, Wu J, Liu JH (2014) Molecular cloning and characterization of CrNCED1, a gene encoding 9-cis-epoxycarotenoid dioxygenase in Citrus reshni, with functions in tolerance to multiple abiotic stresses. Planta 239:61–77
• Xie Z, Duan L, Tian X, Wang B, Eneji AE, Li Z (2008) Coronatine alleviates salinity stress in cotton by improving the antioxidative defense system and radical-scavenging activity. J Plant Physiol 165:375–384
• Xiong L, Schumaker KS, Zhu JK (2002) Cell signaling during cold, drought, and salt stress. Plant Cell 14:S165–S183
• Yadav NS, Shukla PS, Jha A, Agarwal PK, Jha B (2012) The SbSOS1 gene from the extreme halophyte Salicornia brachiata enhances Na (+) loading in xylem and confers salt tolerance in transgenic tobacco. BMC Plant Biol 12:188
• Yu H, Chen X, Hong YY, Wang Y, Xu P, Ke SD, Liu HY, Zhu JK, Oliver DJ, Xiang CB (2008) Activated expression of an Arabidopsis HD-START protein confers drought tolerance with improved root system and reduced stomatal density. Plant Cell 20:1134–1151
• Zhao Y, Ma Q, Jin X, Peng X, Liu J, Deng L, Yan H, Sheng L, Jiang H, Cheng B (2014) A novel Maize homeodomain-leucine zipper (HD-Zip) I gene, Zmhdz10, positively regulates drought and salt tolerance in both rice and Arabidopsis. Plant Cell Physiol 55:1142–1156
• Zhou C, Sun YJ, Ma ZY, Wang JF (2015) Heterologous expression of EsSPDS1 in tobacco plants improves drought tolerance with efficient reactive oxygen species scavenging systems. S Afr J Bot 96:19–28
• Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:247–273
• Zhu L, Guo J, Zhu J, Zhou C (2014) Enhanced expression of EsWAX1 improves drought tolerance with increased accumulation of cuticular wax and ascorbic acid in transgenic Arabidopsis. Plant Physiol Biochem 75:24–35

Identyfikatory

DOI

10.1007/s11738-015-1863-2