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2007 | 29 | 2 |
Tytuł artykułu

Stress-induced expression of cucumber chitinase and Nicotiana plumbaginifoliabeta-1,3-glucanase genes in transgenic potato plants

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The genes encoding for a cucumber class III chitinase and Nicotiana plumbaginifolia class I glucanase were co-introduced into Slovak potato (Solanum tuberosum L.) cultivar ETA using Agrobacterium tumefaciens. Expression of both genes was driven by wound-inducible polyubiquitin promoter isolated from Slovak potato breeding line 116/86. Analyses showed inducible, peel-specific expression of both transgenes under stress conditions. The effect of transgene expression on fungal susceptibility of transformants was evaluated in vitro and in vivo. Experiments with crude protein extracts isolated from transgenic microtubers showed growth inhibition of Rhizoctonia solani hyphae in the range from 7.3 to 14.2%. In contrast, experiments performed in growth chamber conditions revealed that the polyubiquitin promoter driven transgene expression did not ensure any obvious increase of transgenic potato resistance against Rhizoctonia solani.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
29
Numer
2
Opis fizyczny
p.133-141,fig.,ref.
Twórcy
  • Institute of Plant Genetics and Biotechnology SAS, Akademicka 2, P.O. Box 39A, 950 07 Nitra, Slovak Republic
autor
  • Institute of Plant Genetics and Biotechnology SAS, Akademicka 2, P.O. Box 39A, 950 07 Nitra, Slovak Republic
autor
  • The Potato Research and Breeding Institute, Popradska 518, Velka Lomnica 059 52, Slovak Republic
autor
  • Institute of Plant Genetics and Biotechnology SAS, Akademicka 2, P.O. Box 39A, 950 07 Nitra, Slovak Republic
autor
  • Slovak Agricultural Research Authority, Hlohovska 2, 949 92 Nitra, Slovak Republic
  • Institute of Plant Genetics and Biotechnology SAS, Akademicka 2, P.O. Box 39A, 950 07 Nitra, Slovak Republic
autor
  • Faculty of Biotechnology and Food Sciences, Slovak University of Agricultur, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic
autor
  • Institute of Plant Genetics and Biotechnology SAS, Akademicka 2, P.O. Box 39A, 950 07 Nitra, Slovak Republic
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-36efe445-2aca-47ff-92dd-26a21eca9560
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