Stress-induced expression of cucumber chitinase and Nicotiana plumbaginifoliabeta-1,3-glucanase genes in transgenic potato plants

• Autorzy: Moravcikova J. , Libantova J. , Hedlak J. , Salaj J. , Bauer M. , Matusikova I. , Galova Z. , Mlynarova L.
• Języki publikacji: EN

Abstrakty

EN

The genes encoding for a cucumber class III chitinase and Nicotiana plumbaginifolia class I glucanase were co-introduced into Slovak potato (Solanum tuberosum L.) cultivar ETA using Agrobacterium tumefaciens. Expression of both genes was driven by wound-inducible polyubiquitin promoter isolated from Slovak potato breeding line 116/86. Analyses showed inducible, peel-specific expression of both transgenes under stress conditions. The effect of transgene expression on fungal susceptibility of transformants was evaluated in vitro and in vivo. Experiments with crude protein extracts isolated from transgenic microtubers showed growth inhibition of Rhizoctonia solani hyphae in the range from 7.3 to 14.2%. In contrast, experiments performed in growth chamber conditions revealed that the polyubiquitin promoter driven transgene expression did not ensure any obvious increase of transgenic potato resistance against Rhizoctonia solani.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2007
• Tom: 29
• Numer: 2
• Opis fizyczny: p.133-141,fig.,ref.

Twórcy

autor

Moravcikova J.

• Institute of Plant Genetics and Biotechnology SAS, Akademicka 2, P.O. Box 39A, 950 07 Nitra, Slovak Republic

autor

Libantova J.

• Institute of Plant Genetics and Biotechnology SAS, Akademicka 2, P.O. Box 39A, 950 07 Nitra, Slovak Republic

autor

Hedlak J.

• The Potato Research and Breeding Institute, Popradska 518, Velka Lomnica 059 52, Slovak Republic

autor

Salaj J.

• Institute of Plant Genetics and Biotechnology SAS, Akademicka 2, P.O. Box 39A, 950 07 Nitra, Slovak Republic

autor

Bauer M.

• Slovak Agricultural Research Authority, Hlohovska 2, 949 92 Nitra, Slovak Republic

autor

Matusikova I.

• Institute of Plant Genetics and Biotechnology SAS, Akademicka 2, P.O. Box 39A, 950 07 Nitra, Slovak Republic

autor

Galova Z.

• Faculty of Biotechnology and Food Sciences, Slovak University of Agricultur, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic

autor

Mlynarova L.

• Institute of Plant Genetics and Biotechnology SAS, Akademicka 2, P.O. Box 39A, 950 07 Nitra, Slovak Republic

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