Molecular analysis of a fragment of gene E1B 19K of canine adenovirus 2 (CAV-2) isolated from dogs with symptoms of cough
Treść / Zawartość
The aim of this study was to perform molecular analysis of canine adenovirus 2 (CAV-2) E1B 19K gene fragment isolated from 20 dogs of various breeds (12 males and 8 females aged 1-9 years), with clinical symptoms of upper respiratory tract infections, from the Lubelszczyzna region. Nasal swabs were taken from dogs. DNA of CAV-2 was detected using the PCR method in 16 swabs. All PCR products were sequenced, and the obtained sequences were compared with each other and with the sequence of the E1B 19K gene of the CAV-2 strain from an online database of NCBI GenBank: AC 000003. Based on analysis of the obtained sequences, three polymorphic variants of CAV-2 (No.1-3) with homology of 78 - 100% were distinguished. The nucleotide and amino acid sequences of the most frequently represented polymorphic variant, No. 1, differed from the sequences of polymorphic variant No. 2 with one substitution. The nucleotide and amino acid sequence of the E1B 19K gene of CAV-2 AC 000003 differed from the analogous sequences of representatives of variant No. 1 with 44 nucleotide and 19 amino acid substitutions. The small number of nucleotide differences in the E1B 19K CAV-2 gene among the examined own isolates, compared with AC 000003, suggest that the infections in dogs were caused by a relatively genetically stable virus which occurs in eastern Poland.
- Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences Lublin, Gleboka 30, 20-612 Lublin, Poland
- Appel M, Binn LN (1987) Canine infectious tracheobronchitis. Short review: kennel cough. In: Appel MJ (ed) Virus infections of carnivores. Elsevier Science Publisher, Amsterdam, pp 201-211.
- Appel MJG, Pickerill RH, Menegus M, Percy DH, Parsonson IM, Sheffy BE (1970) Current status of canine respiratory disease. Procedings of the 20th Gaines Veterinary Symposium, Manhattan, USA, pp 15-23.
- Benetka V, Weissenbock H, Kudielka I, Pallan C, Rothmuller G, Mostl K (2006) Canine adenovirus type 2 infection in four puppies with neurological signs. Vet Rec 158: 91-94.
- Buonavoglia C, Martella V (2007) Canine respiratory viruses. Vet Res 38: 355-373.
- Cardoso FM, Kato SE, Huang W, Flint SJ, Gonzalez RA (2008) An early function of the adenoviral E1B 55 kDa protein is required for the nuclear relocalization of the cellular p53 protein in adenovirus-infected normal human cells. Virology 378: 339-346.
- Chaturvedi U, Tiwari AK, Ratta B, Ravindra PV, Rajawat YS, Palia SK, Rai A (2008) Detection of canine adenoviral infections in urine and faeces by the polymerase chain reaction. J Virol Methods 149: 260-263.
- Davison AJ, Benko M, Harrach B (2003) Genetic content and evolution of adenoviruses. J Gen Virol 84: 2895-2908.
- Ditchfield J, Macpherson LW, Zbitnew A (1962) Association of a canine adenovirus (Toronto A 26/61) with an outbreak of laryngotracheitis (“kennel cough”). A preliminary report. Can Vet J 3: 238-247.
- Fairchild GA, Cohen D (1969) Serologic study of a canine adenovirus (Toronto A26/61) infection in dogs. Am J Vet Res 30: 923-928.
- Flaschker N, Feyen O, Fend S, Simon E, Schadewaldt P, Wendel U (2007) Description of the mutations in 15 subjects with variant forms of maple syrup urine disease. J Inherit Metab Dis 30: 903-909.
- Gerhold RW, Allison AB, Temple DL, Chamberlain MJ, Strait KR, Keel MK (2007) Infectious canine hepatitis in a gray fox (Urocyon cinereoargenteus). J Wildl Dis 43: 734-736.
- Hamelin C, Jouvenne P, Assaf R (1985) Association of a type-2 canine adenovirus with an outbreak of diarrhoeal disease among a large dog congregation. J Diarrhoeal Dis Res 3: 84-87.
- Hamelin C, Marsolais G, Assaf R (1984) Interspecific differences between the DNA restriction profiles of canine adenoviruses. Experientia 40: 482.
- Harasawa R, Tohya Y, Yoshida M, Kataoka Y, Katae H (1994) Two genomic variations in the E1 region of canine adenovirus type 2 strains. Vet Microbiol 40: 373-378.
- Koncicki A, Krasnodębska-Depta A, Mazur-Gonkowska B (2006) New data concerning the significance of adenovirus in poultry pathogenicity. Med Weter 62: 739-743.
- Lynch JP 3rd, Fishbein M, EchavarriaM(2011) Adenovirus. Semin Respir Crit Care Med 32: 494-511.
- Macartney L, Cavanagh HM, Spibey N (1988) Isolation of canine adenovirus-2 from the faeces of dogs with enteric disease and its unambiguous typing by restriction endonuclease mapping. Res Vet Sci 44: 9-14.
- Marusyk RG (1972) Comparison of the immunological properties of two canine adenoviruses. Can J Microbiol 18: 817-823.
- Marusyk RG, Norrby E, Lundqvist U (1970) Biophysical comparison of two canine adenoviruses. J Virol 5: 507-512.
- Morrison MD, Onions DE, Nicolson L (1997) Complete DNA sequence of canine adenovirus type 1. J Gen Virol 78: 873-878.
- Rao L, Modha D, White E (1997) The E1B 19K protein associates with lamins in vivo and its proper localization is required for inhibition of apoptosis. Oncogene 15: 1587-1597.
- Schreiner S, Wimmer P, Groitl P, Chen SY, Blanchette P, Branton PE, Dobner T (2011) Adenovirus type 5 early region 1B 55K oncoprotein-dependent degradation of cellular factor Daxx is required for efficient transformation of primary rodent cells. J Virol 85: 8752-8765.
- Spurgeon ME, Ornelles DA (2009) The adenovirus E1B 55-kilodalton and E4 open reading frame 6 proteins limit phosphorylation of eIF2alpha during the late phase of infection. J Virol 83: 9970-9982.
- Swango LJ, Eddy GA, Binn LN (1969) Serologic comparisons of infectious canine hepatitis and Toronto A26/61 canine adenoviruses. Am J Vet Res 30: 1381-1387.
- Truszczyński M, Pejsak Z (2012) Diarrheal diseases in pigs caused by facultatively pathogenic viruses. Med Weter 68: 9-14.
- White E, Denton A, Stillman B (1988) Role of the adenovirus E1B 19,000-dalton tumor antigen in regulating early gene expression. J Virol 62: 3445-3454.
- Wieliczko A, Tomanek B, Kuczkowski M (2003) Prevalence of infectious diseases in ring-necked pheasant flocks in Poland. Pol J Vet Sci 6: 177-182.
- Wiśniewski E (2004) Adenoviral infections in horse. Med Weter 60: 1139-1142.
- Yamamoto R, Marusyk RG (1968) Morphological studies of a canine adenovirus. J Gen Virol 2: 191-194.