Toll-like receptor-4, but not toll-like receptor-2 mediates secretion of tumour necrosis factor alpha and interleukin-8 in lipopolysaccharide-stimulated mouse mammary epithelial cells

• Autorzy: He C.-L. , Yi Q. , Li Y. F. , Yang H. , Wang L.
• Języki publikacji: EN

Abstrakty

EN

Mammary epithelial cells (MECs) from Kunming mice were isolated and stimulated in vitro with 10 µg/mL of Escherichia coli lipopolysaccharide (LPS). The release of tumour necrosis factor α (TNF-α) and interleukin-8 (IL-8) into culture supernatants was measured by ELISA. Furthermore, blocking experiments with Toll-like receptor 2 (TLR2) and TLR4 antibodies were performed to verify whether cytokine secretion depended on LPS-induced activation of TLR2 or TLR4. The results revealed that LPS-stimulated mouse MECs significantly secreted TNF-α and IL-8. Blocking of the TLR4 pathway inhibited the secretion of TNF-α and IL-8, while inhibition of LPS-induced TNF-α and IL-8 production was not observed when TLR2 was blocked. Thus, TLR4 can mediate the LPS-induced expression of cytokines such as TNF-α and IL-8 in mouse MECs.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2013
• Tom: 57
• Numer: 3
• Opis fizyczny: p.393-397,fig.,ref.

Twórcy

autor

He C.-L.

• Department of Pharmacy, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, Sichuan, PR China

autor

Yi Q.

• Department of Veterinary Medicine, College of Animal Science, Guizhou University, Guiyang 550025, Guizhou, PR Chin

autor

Li Y. F.

• Department of Veterinary Medicine, College of Animal Science, Guizhou University, Guiyang 550025, Guizhou, PR Chin

autor

Yang H.

• Department of Veterinary Medicine, College of Animal Science, Guizhou University, Guiyang 550025, Guizhou, PR Chin

autor

Wang L.

• Biochemistry Engineering Center of Guizhou Province, Guizhou University, Guiyang 550025, Guizhou, PR China

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Identyfikatory

DOI

10.2478/bvip-2013-0068