A simplified method for generating oligodendrocyte progenitor cells from neural precursor cells isolated from the E16 rat spinal cord

• Autorzy: Fu S.-L. , Hu J.-G. , Li Y. , Wang Y.-X. , Jin J.-Q. , Xu X.-M. , Lu P.-H.
• Języki publikacji: EN

Abstrakty

EN

Conditioned medium obtained from B104 neuroblastoma cells (B104CM) has been used widely for inducing oligodendrocyte progenitor cells (OPCs) from neural precursor cells (NPCs). Our previous studies have demonstrated that E16 rat spinal cord-derived NPCs could be induced to differentiate into OPCs using a combination of B104CM and basic fibroblast growth factor (bFGF). Here we report the development of a more efficient and reliable approach to generate large quantities of highly purified OPCs from spinal cord-derived NPCs using a combination of platelet derived growth factor (PDGF) and bFGF. We demonstrated that, after the two factors application, over 90% cells displayed typical bipolar or tripolar morphology and expressed markers for OPCs including A2B5 (90.36 +/- 4.59%), NG2 (93.63 +/- 3.37%) and platelet derived growth factor alpha receptor (PDGFR; 90.35 +/- 1.95%). Our results indicated that the PDGF/bFGF combination is more efficient in generating OPCs than the B104CM/bFGF. And it is a more potent combination of factors in promoting proliferation of OPCs.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2007
• Tom: 67
• Numer: 4
• Opis fizyczny: p.367-377,fig.,ref.

Twórcy

autor

Fu S.-L.

• Department of Neurobiology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, PR China

autor

Hu J.-G.

• Department of Neurobiology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, People's Republic of China

autor

Li Y.

• Department of Neurobiology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, People's Republic of China

autor

Wang Y.-X.

• Department of Neurobiology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, People's Republic of China

autor

Jin J.-Q.

• Department of Neurobiology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, People's Republic of China

autor

Xu X.-M.

• Department of Neurobiology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, People's Republic of China
• University of Louisville School of Medicine, Louisville, KY 40292, USA

autor

Lu P.-H.

• Department of Neurobiology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, People's Republic of China

Uwagi

PL

Rekord w opracowaniu