Preclinical characteristics and regenerative potential of adipose – derived MSC

• Autorzy: Sarnowska A. , Figiel-Dabrowska A. , Szczepanik E. , Kuzma M. , Mierzewska H. , Antczak-Marach D. , Kruk M. , Terczynska I. , Krzesniak N. , Sawicka E. , Nowak A. , Noszczyk B. , Kaminska A. , Marchel A. , Domanska-Janik K.
• Języki publikacji: EN

Abstrakty

EN

While the rapid development of stem cell-based therapies are nowadays running, the reliable protocols leading to safe and effective way for cell isolation, expansion and commitment in vitro according to distinguished therapeutic purposes still need more consensus and standardization. In order to obtain particular disease-committed therapeutic cells we have screened various culture conditions, especially new systems involving lowered oxygen tension and small molecule treatments which may “rejuvenates” MSC. We also looked on the changes in grow dynamics of long-term cultures in various oxygen concentrations to dissect changes that predict genetic instability of cultivated cells. The standardization of such type of culture (by additional criteria beyond the framework developed by ISCT) should further enhance life-span, expansion and differentiation potential of MSCs needed for more effective regeneration of diseased brain. In recent years we have also entered into the clinic with individual medical experiments (in accordance with the guidelines described by A. Korczyn’ Sieratzki – Chair of Neurology Tel Aviv University 2010) based on mesenchymal regenerative cell therapy to elaborate save therapeutic procedures for autoimmune epilepsy, ALS and vast nerve injury. First results are promising and indicate regenerative and immunomodulatory properties of transplanted MSC. FINANCIAL SUPPORT: The work was supported by National Centre for Research and Development grant No STRATEGMED 1/234261/2/NCBR/2014.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2017
• Tom: 77
• Numer: Suppl.1
• Opis fizyczny: p.41

Twórcy

autor

Sarnowska A.

• Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland

autor

Figiel-Dabrowska A.

• Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland

autor

Szczepanik E.

• The Institute of Mother and Child, Warsaw, Poland

autor

Kuzma M.

• Medical University of Warsaw, Poland

autor

Mierzewska H.

• The Institute of Mother and Child, Warsaw, Poland

autor

Antczak-Marach D.

• The Institute of Mother and Child, Warsaw, Poland

autor

Kruk M.

• The Institute of Mother and Child, Warsaw, Poland

autor

Terczynska I.

• The Institute of Mother and Child, Warsaw, Poland

autor

Krzesniak N.

• Centre of Postgraduate Medical Education, Warsaw, Poland

autor

Sawicka E.

• The Institute of Mother and Child, Warsaw, Poland

autor

Nowak A.

• Medical University of Warsaw, Poland

autor

Noszczyk B.

• Centre of Postgraduate Medical Education, Warsaw, Poland

autor

Kaminska A.

• Medical University of Warsaw, Poland

autor

Marchel A.

• Medical University of Warsaw, Poland

autor

Domanska-Janik K.

• Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland