Development of a RAPD-based male-specific molecular marker in Japanese hop (Humulus japonicus Siebold and Zucc.)

• Autorzy: Mizia P. , Cygan M. , Denysenko M. , Kwolek D. , Chramiec-Glabik A. , Grabowska-Joachimiak A. , Joachimiak A.J.
• Języki publikacji: EN

Abstrakty

EN

The male-specific DNA markers are very useful in molecular sexing of non-flowering plants and seeds of dioecious species. In this paper we identified ten Y chromosome-specific RAPD primers suitable for identification of male plants in three Cannabaceae species with sex chromosomes (Humulus lupulus, XX/XY; H. japonicus, XX/ XY1Y2; Cannabis sativa, XX/XY). Basing on the nucleotide sequence of the OPJ-09 RAPD product we developed the HJY09 SCAR marker, which is very efficient in sexing of Japanese hop.

Słowa kluczowe

EN

Cannabaceae; Japanese hop; Humulus japonicus; SCAR marker; random amplified polymorphic DNA analysis; RAPD marker; molecular sexing; molecular analysis

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2016
• Tom: 58
• Numer: 2
• Opis fizyczny: p.21-27,fig.,ref.

Twórcy

autor

Mizia P.

• Department of Plant Cytology and Embryology, Institute of Botany, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland

autor

Cygan M.

• Department of Plant Cytology and Embryology, Institute of Botany, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland

autor

Denysenko M.

• Department of Plant Cytology and Embryology, Institute of Botany, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland

autor

Kwolek D.

• Department of Plant Cytology and Embryology, Institute of Botany, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland

autor

Chramiec-Glabik A.

• Department of Plant Cytology and Embryology, Institute of Botany, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland

autor

Grabowska-Joachimiak A.

• Department of Plant Breeding and Seed Science, University of Agriculture in Krakow, Lobzowska 24, 31-140 Krakow, Poland

autor

Joachimiak A.J.

• Department of Plant Cytology and Embryology, Institute of Botany, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland

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Identyfikatory

DOI

10.1515/abcsb-2016-001