EN
Ovulation rate, prolificacy, plasma IGF-I and steroid hormones concentrations were compared amoung two breeds of Polish ewes exposed to hCG. Both low (PLS) and high prolific group (BCP) ewes were separated randomly into three subgroups (10 ewes each): control animals injected with an equal volume of saline solution, once treated with hCG (300 IU, i.m.) on the 15th day of the oestrus cycle, and twice treated with 300 IU, i.m hCG on the 13th and 15th days of the oestrus cycle. Ovulation rate for once treated PLS and BCP groups (2.6 and 2.9 respectively) were always higher than in double treated groups (2.0 and 2.4 respectively). Stimulatory influence of hCG on ovulation rate was more pronounced in less prolific PLS breed than in BCP sheep. The prolificacy was augmented to a considerably greater extent in ewes exposed to double treatment with hCG, with significant differences noted only in BCP ewes. In all ewes used in this experiment, the number of ovulatory follicles rose together with the increment of plasma IGF-I concentration. The most influencal level of IGF-I on the number of ovulatory follicles following hCG treatment ranged between 28.0-31.0 ng/ml. The obtained results indicate that optimal time to induce extra ovulatory response to hCG is the beginning of estrus, whereas hCG given at the end of estrus time is effective in improving prolificacy in ewes.