A multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines

• Autorzy: Hu L. , Lin X.Y. , Yang Z.X. , Yao X.P. , Li G.L. , Peng S.Z. , Wang Y.
• Języki publikacji: EN

Abstrakty

EN

In this assay, we developed and evaluated a multiplex PCR (mPCR) for its ability in detecting multiple infections of swine simultaneously. Four pairs of primers were used to detect five viruses. Specific primers were designed for classical swine fever virus (CSFV), African swine fever virus (ASFV) and pseudorabies (PRV). A pair of primers was designed prudently for two different types of porcine reproductive and respiratory syndrome virus that respectively were porcine reproductive and respiratory syndrome virus (PRRSV), highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). The detection limits of the mPCR were 1.09×10⁴, 1.50×10³, 2.10×10³, 1.30×10³ and 8.97×10² copies/reaction for CSFV, ASFV, HP-PRRSV, PRRSV and PRV, respectively. A total of 49 clinical specimens were tested by the mPCR, and the result showed that co-infection by two or three viruses was 51%. In conclusion, the PCR is a useful tool for clinical diagnosis of not only single infections but also mixed infections in swines.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2015
• Tom: 18
• Numer: 4
• Opis fizyczny: p.715-723,fig.,ref.

Twórcy

autor

Hu L.

• Animal Quarantine Laboratory,College of Veterinary Medicine, Sichuan Agricultural University, Chengdu Sichuan 611130, China

autor

Lin X.Y.

• Animal Quarantine Laboratory,College of Veterinary Medicine, Sichuan Agricultural University, Chengdu Sichuan 611130, China

autor

Yang Z.X.

• Animal Quarantine Laboratory,College of Veterinary Medicine, Sichuan Agricultural University, Chengdu Sichuan 611130, China

autor

Yao X.P.

• Animal Quarantine Laboratory,College of Veterinary Medicine, Sichuan Agricultural University, Chengdu Sichuan 611130, China

autor

Li G.L.

• Animal Quarantine Laboratory,College of Veterinary Medicine, Sichuan Agricultural University, Chengdu Sichuan 611130, China

autor

Peng S.Z.

• Animal Quarantine Laboratory,College of Veterinary Medicine, Sichuan Agricultural University, Chengdu Sichuan 611130, China

autor

Wang Y.

• Animal Quarantine Laboratory,College of Veterinary Medicine, Sichuan Agricultural University, Chengdu Sichuan 611130, China
• Key laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu Sichuan 611130, China

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Identyfikatory

DOI

10.1515/pjvs-2015-0093