Microsatellite polymorphism in the study of genetic diversity of an experimental flock of Ayam Cemani breed

• Autorzy: Gruszczynska J. , Lukasiewicz M.
• Języki publikacji: EN

Abstrakty

EN

Microsatellite polymorphism in the study of genetic diversity of an experimental flock of Ayam Cemani breed. Blood samples were collected from pterygoidal vein of 53 birds (29 cocks and 24 hens) come from the experimental flock of Ayam Cemani hens and underwent molecular analysis. The phenol-chloroform method was used to extract genomic DNA from collected blood. Microsatellite sequences have been commonly used in molecular analysis of animal origin. On the basis of literature, 5 microsatellite sequences were chosen for further molecular analysis: MCW0210, MCW0184, LEI0071, MCW0145 and ADL0306. The automatic sequencer ALF Express (Pharmacia LKB) was used to genotype birds. Among all examined loci polymorphism was detected. Approximate analyzed microsatellite sequences lengths were: 156-168bp for MCW0210 (3 alleles); 240 and 260bp for MCW0184; 280-300bp for LEI0071 (3 alleles); 206-218bp for MC WO 145 (4 alleles) and 119-125bp for ADL0306 (3 alleles). The highest level of homozygosity was found as follows for MCW0184 (about 81%), the lowest one for ADL0306 (4%). The high level of Hₒ (0.19-0.96), HE (0.41-0.64) and PIC (0.32-0.56) were calculated and compared as not so high according to other scientific reports.

PL

Do badań krew pobrano z żyły skrzydłowej od 53 ptaków (29 kogutów i 24 kur) rasy Ayam Cemani pochodzących ze stada doświadczalnego. Genomowy DNA izolowano z krwi metodą fenolowo- hloroformową. W analizie molekularnej wykorzystano 5 sekwencji mikrosatelitarnych: MCW0210, MCW0184, LEI0071, MCW0145 i ADL0306. Ptaki zgenotypowano przy wykorzystaniu automatycznego sekwenatora ALF Express (Pharmacia LKB). Stwierdzono występowanie polimorfizmu we wszystkich badanych loci. Długość analizowanych sekwencji mikrostaelitarnych była następująca: 156-168pz dla MCW0210 (3 allele); 240pz i 260pz dla MCW0184; 280-300pz dla LEI0071 (3 allele); 206-218pz dla MCW0145 (4 allele) i 119-125 pz dla ADL0306 (3 allele). Najwyższą homozygotyczność odnotowano pod względem sekwencji MCW0184 (około 81%), a najniższą dla ADL0306 (4%). Wielkość współczynników Hₒ (0,19-0,96), HE (0,41-0,64) oraz wskaźnika PIC (0,32-0,56) nie była tak wysoka jak w badaniach innych autorów.

• Wydawca: -
• Czasopismo: Annals of Warsaw University of Life Sciences - SGGW. Animal Science
• Rocznik: 2010
• Tom: 47
• Opis fizyczny: p.39-44,ref.

Twórcy

autor

Gruszczynska J.

• Department of Genetics and Animal Breeding, Warsaw University of Life Sciences – SGGW, Ciszewskiego 8, 02-786 Warsaw, Poland

autor

Lukasiewicz M.

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