Effect of sodium chloride-induced stress on growth, proline, glycine betaine accumulation, antioxidative defence and bacoside A content in in vitro regenerated shoots of Bacopa monnieri (L.) Pennell

• Autorzy: Ahire M L , Walunj P. R. , Kishor P. B. K. , Nikam T. D.
• Języki publikacji: EN

Abstrakty

EN

Growth, osmotic adjustment, antioxidant enzyme defense and the principle medicinal component bacoside A were studied in the in vitro raised shoot cultures of Bacopa monnieri, a known medicinal plant, under different concentrations of NaCl [0.0 (control), 50, 100, 150 or 200 mM]. A sharp increase in Na+ content was observed at 50 mM NaCl level and it was about 6.4-fold higher when compared with control. While Na+ content increased in the shoots with increasing levels of NaCl in the medium, both K+ and Ca2+ concentrations decreased. Significant reduction was observed in shoot number per culture; shoot length, fresh weight (FW), dry weight (DW) and tissue water content (TWC) when shoots were exposed to increasing NaCl concentrations (50–200 mM) as compared with the control. Decrease in TWC was not significant at higher NaCl level (150 and 200 mM). At 200 mM NaCl, growth of shoots was adversely affected and microshoots died under prolonged stress. Minimum damage to the membrane as assessed by malondialdehyde (MDA) content was noticed in the controls in contrast to sharp increase of it in NaCl-stressed shoots. Higher amounts of free proline, glycinebetaine and total soluble sugars (TSS) accumulated in NaCl-stressed shoots indicating that it is a glycinebetaine accumulator. About 2.11-fold higher H2O2 content was observed at 50 mM NaCl as compared with control and it reached up to 7.1-folds more at 200 mM NaCl. Antioxidant enzyme activities (superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidase) also increased with a rise in NaCl level. Increase in bacoside A, a triterpene saponin content was observed only up to 100 mM NaCl level. Higher salt concentrations inhibited the accumulation of bacoside A. It appears from the data that accumulation of osmolytes, ions and elevated activities of antioxidant enzymes play an important role in osmotic adjustment in shoot cultures of Bacopa under salt stress.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 06
• Opis fizyczny: p.1943-1953,fig.,ref.

Twórcy

autor

Ahire M L

• Department of Botany, University of Pune, Pune 411 007, India

autor

Walunj P. R.

• Department of Botany, University of Pune, Pune 411 007, India

autor

Kishor P. B. K.

• Department of Genetics, Osmania University, Hyderabad 500 007, India

autor

Nikam T. D.

• Department of Botany, University of Pune, Pune 411 007, India

Bibliografia

• Abraham G, Dhar DW (2010) Induction of salt tolerance in Azolla microphylla Kaulf through modulation of antioxidant enzymes and ion transport. Protoplasma 245:105–111
• Ahire ML, Patil PP, Kavi Kishor PB, Nikam TD (2012) Micropropagation and assessment of antibiotic selection in vitro of Bacopa monnieri (L.) Pennell. Int J Plant Dev Biol 6:34–39
• Ajungla L, Patil PP, Barmukh RB, Nikam TD (2009) Influence of biotic and abiotic elicitors on accumulation of hyoscyamine and scopolamine in root cultures of Datura metel L. Ind J Biotechnol 8:317–322
• Alexieva V, Sergiev I, Mapelli S, Karanov E (2001) The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant Cell Environ 24:1337–1344
• Ali G, Srivastava PS, IqbalM(1998a) Effect of cadmium and copper on growth of Bacopa monniera regenerants. Biol Plant 41:635–639
• Ali G, Srivastava PS, Iqbal M (1998b) Morphogenic response and proline content in Bacopa monniera cultures grown under copper stress. Plant Sci 138:191–195
• Ali G, Srivastava PS, Iqbal M (1999) Proline accumulation, protein pattern and photosynthesis in Bacopa monniera regenerants grown under NaCl stress. Biol Plant 42:89–95
• Ali G, Srivastava PS, Iqbal M (2000) Influence of cadmium and zinc on growth and photosynthesis of Bacopa monniera cultivated in vitro. Biol Plant 43:599–601
• Ali MB, Hahn EJ, Paek KY (2006) Copper-induced changes in the growth, oxidative metabolism and saponin production in suspension culture roots of Panax ginseng in bioreactors. Plant Cell Rep 25:1122–1132
• Anbarasi K, Vani G, Balakrishna K, Shyamala Devi CS (2005) Effect of bacoside A on membrane-bound ATPases in the brain of rats exposed to cigarette smoke. J Biochem Mol Toxicol 19:59–65
• Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol 24:1–15
• Bajji M, Kintet JM, Lutts S (1998) Salt stress effects on roots and leaves of Atriplex halimus L. and their corresponding callus cultures. Plant Sci 137:131–142
• Basu S, Gangopadhyay G, Mukherjee BB (2002) Salt tolerance in rice in vitro: implication of accumulation of Na+, K+ and proline. Plant Cell Tiss Org Cult 69:55–64
• Bates L, Waldren RP, Teare JD (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207
• Becana M, Moran JF, Iturbe-Ormaetxe I (1986) Iron-dependent oxygen free radical generation in plants subjected to environmental stress: toxicity and antioxidant protection. Plant Soil 201:137–147
• Binzel ML, Reuveni M (1994) Cellular mechanisms of salt tolerance in plant cells. Hort Rev 16:33–69
• Bohnert HJ, Jensen RG (1996) Strategies for engineering water-stress tolerance in plants. Trends Biotechnol 14:89–97
• Bor M, Özdemir F, Türkan I (2003) The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar beet Beta vulgaris L. and wild beet Beta maritima L. Plant Sci 164:77–84
• Bowler C, Van Montagu M, Inze D (1992) Superoxide dismutase and stress tolerance. Annu Rev Plant Physiol Plant Mol Biol 43:83–116
• Bracci T, Minnocci A, Sebastiani L (2008) In vitro olive (Olea europaea L.) cvs Frantoio and Moraiolo microshoot tolerance to NaCl. Plant Biosys 142:563–571
• Cakmak I, Marschner H (1992) Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves. Plant Physiol 98:1222–1227
• Cano EA, Pérez-Alfocea F, Moreno V, Caro M, Bolarin MC (1998) Evaluation of salt tolerance in cultivated and wild tomato species through in vitro shoot apex culture. Plant Cell Tiss Org Cult 53:19–26
• Carpin S, Quelhazi L, Filali M, Cheniux JX, Rideau M, Hamdi S (1997) The relationship between the accumulation of 28 KD polypeptide and that of indole alkaloids in Catharanthus roseus cell suspension cultures. J Plant Physiol 150:452–457
• Chen THH, Murata N (2002) Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes. Curr Opin Plant Biol 5:250–257
• Cherian S, Reddy MP (2003) Evaluation of NaCl tolerance in the callus cultures of Suaeda nudiflora Moq. Biol Plant 46:193–198
• Chinnusamy V, Jagendorf A, Zhu JK (2005) Understanding and improving salt tolerance in plants. Crop Sci 45:437–448
• Debnath M (2008) Responses of Bacopa monnieri to salinity and drought stress in vitro. J Med Plants Res 2:347–351
• Deepak M, Amit A (2004) The need for establishing identities of bacoside A, and B, the putative major bioactive saponins of Indian medicinal plant Bacopa monnieri. Phytomed 11:264–268
• Errabii T, Gandonou CB, Essalmani H, Abrini J, Idaomor M, Senhaji NS (2007) Effects of NaCl and mannitol induced stress on sugarcane (Saccharum sp.) callus cultures. Acta Physiol Plant 29:95–102
• Flors V, Paradıs M, Garcıa-Andrade J, Cerezo M, González-Bosch C (2007) A tolerant behavior in salt-sensitive tomato plants can be mimicked by chemical stimuli. Plant Sign Behav 2:50–57
• Flowers TJ (2004) Improving crop salt tolerance. J Exp Bot 55:307–319
• Ghorpade RP, Chopra A, Nikam TD (2011) Influence of biotic and abiotic elicitors on four major isomers of boswellic acid in callus culture of Boswellia serrata Roxb. Plant Omics J 4:169–176
• Grieve CM, Grattan SR (1983) Rapid assay for determination of water soluble quaternary ammonium compounds. Plant Soil 70:303–307
• Halbrock K, Grisebach H (1979) Enzymic controls in the biosynthesis of lignin and flavonoids. Ann Rev Plant Physiol 30:105–130
• Heath RL, Packer L (1968) Photooxidation in isolated chloroplast. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
• Hemeda HM, Klein BP (1990) Effects of naturally occurring antioxidants on peroxidase activity of vegetable extracts. J Food Sci 55:184–185
• Jaleel CA, Manivannan P, Lakshmanan GMA, Sridharan R, Panneerselvam R (2007) NaCl as a physiological modulator of proline metabolism and antioxidant potential in Phyllanthus amarus. C R Biol 330:806–813
• Jin H, Sun Y, Yang Q, Chao Y, Kang J, Jin H, Li Y, Margaret G (2010) Screening of genes induced by salt stress from Alfalfa. Mol Biol Rep 37:745–753
• Jithesh MN, Prashanth SR, Sivaprakash KR, Parida AK (2006) Antioxidative response mechanisms in halophytes: their role in stress defence. J Genet 85:237–254
• Kavi Kishor PB, Sangam S, Amrutha RN, Sri Laxmi P, Naidu KR, Rao KRS, Rao Sreenath, Reddy KJ, Theriappan P, Sreenivasulu P (2005) Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: Its implications in plant growth and abiotic stress tolerance. Curr Sci 88:424–438
• Kim SY, Lim JH, Park MR, Kim YJ, Park TH, Sco YW, Choi KG, Yun SJ (2005) Enhanced antioxidant enzymes are associated with reduced hydrogen peroxide in barley roots under saline stress. J Biochem Mol Biol 38:218–224
• Kishore K, Singh M (2005) Effect of bacosides, a alcoholic extract of Bacopa monnieri Linn. (brahmi), on experimental amnesia in mice. Indian J Exp Biol 43:640–645
• Koca H, Bor M, O¨ zdemir F, Tu¨rkan I (2007) The effect of salt stress on lipid peroxidation, antioxidative enzymes and proline content of sesame cultivars. Environ Exp Bot 60:344–351
• Kumar V, Shriram V, Nikam TD, Jawali N, Shitole MG (2008) Sodium chloride-induced changes in mineral nutrients and proline accumulation in indica rice cultivars differing in salt tolerance. J Plant Nutr 31:1999–2017
• Kumar V, Shriram V, Nikam TD, Jawali N, Shitole MG (2009) Antioxidant enzyme activities and protein profiling under salt stress in indica rice genotypes differing in salt tolerance. Arch Agron Soil Sci 55:379–394
• Lokhande VH, Nikam TD, Suprasanna P (2010) Biochemical, physiological and growth changes in response to salinity in callus cultures of Sesuvium portulacastrum L. Plant Cell Tiss Org Cult 102:17–25
• Lokhande VH, Nikam TD, Patade VY, Ahire ML, Suprasanna P (2011) Effects of optimal and supra-optimal salinity stress on antioxidative defence, osmolytes and in vitro growth responses in Sesuvium portulacastrum L. Plant Cell Tiss Org Cult 104:41–49
• Lowry OH, Roenbrough NJ, Farr AL, Randal EJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
• Ma L, Li Y, Yu C, Wang Y, Li X, Li N, Chen Q, Bu N (2012) Alleviation of exogenous oligochitosan on wheat seedlings growth under salt stress. Protoplasma 249:393–399
• Mallik S, Nayak M, Sahu BB, Panigrahi AK, Shaw BP (2011) Response of antioxidant enzymes to high NaCl concentration in different salt-tolerant plants. Biol Plant 55:191–195
• Mandhania S, Madan S, Sawhney V (2006) Antioxidant defense mechanism under salt stress in wheat seedlings. Biol Plant 50:227–231
• Mills D, Zhang G, Benzioni A (2001) Effect of different salts and of ABA on growth and mineral uptake in Jojoba shoots grown in vitro. J Plant Physiol 158:1031–1039
• Mittal S, Kumari N, Sharma V (2012) Differential response of salt stress on Brassica juncea: photosynthetic performance, pigment, proline, D1 and antioxidant enzymes. Plant Physiol Biochem 54:17–26
• Mittova V, Tal M, Volokita M, Guy M (2002) Salt stress induces up regulation of an efficient chloroplast antioxidant system in the salt-tolerant wild tomato species Lycopersicon pennellii but not in the cultivated species. Physiol Plant 115:393–400
• Moseki B, Buru JC (2010) Ionic and water relations of Sesuvium portulacastrum (L). Sci Res Essay 5:35–40
• Munns R (2002) Comparative physiology of salt and water stress. Plant, Cell Environ 25:239–250
• Munns R, Tester M (2008) Mechanisms of salinity tolerance. Ann Rev Plant Biol 59:651–681
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol 15:473–497
• Murphy KST, Durako MJ (2003) Physiological effects of short-term salinity changes on Ruppia maritima. Aquat Bot 75:293–309
• Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880
• Nathan PJ, Clarke J, Lloyd J, Hutchinson CW, Downey L, Stough C (2001) The acute effects of an extract of Bacopa monniera (Brahmi) on cognitive function in healthy normal subjects. Human Psychopharmacol 16:345–351
• Nguyen HTT, Shim IS, Kobayashi K, Usui K (2005) Effects of salt stress on ion accumulation and antioxidant enzyme activities of Oryza sativa L. and Echinochloa oryzicola Vasing. Weed Biol Manage 5:1–7
• Noctor G, Foyer CH (1998) Ascorbate and glutathione: keeping active oxygen under control. Ann Rev Plant Physiol Plant Mol Biol 49:249–279
• O’Neill RA, Scott TK (1987) Rapid effects of IAA on cell surface proteins from intact carrot suspension culture cells. Plant Physiol 84:443–446
• Osbourn AE (2003) Saponins in cereals. Phytochem 62:1–4
• Ottow EA, Brinker M, Teichmann T, Fritz E, Kaiser W, Brosché M, Kangasjärvi J, Jiang X, Polle A (2005) Populus euphratica displays apoplastic sodium accumulation, osmotic adjustment by decreases in calcium and soluble carbohydrates, and develops leaf succulence under salt stress. Plant Physiol 139:1762–1772
• Parale A, Barmukh R, Nikam T (2010) Influence of organic supplements on production of shoots and callus biomass and accumulation of bacoside in Bacopa monniera (L.) Pennell. Physiol Mol Biol Plants 16:167–175
• Parida AK, Das AB, Mohanty P (2004) Defense potentials to NaCl in a mangrove, Bruguiera parviflora: differential changes of isoforms of some antioxidative enzymes. J Plant Physiol 161:531–542
• Pérez-Tornero O, Tallón CI, Porras I, Navarro JM (2009) Physiological and growth changes in micropropagated Citrus macrophylla explants due to salinity. J Plant Physiol 166:1923–1933
• Radhakrishnan R, Leelapriya T, Ranjitha Kumari BD (2012) Effects of pulsed magnetic field treatment of soybean seeds on calli growth, cell damage, and biochemical changes under salt stress. Bioelectromagnetics. doi:10.1002/bem.21735
• Raven JA (1985) Regulation of pH and generation of osmolarity in vascular land plants: costs and benefits in relation to efficiency of use of water, energy and nitrogen. New Phytol 101:25–77
• Rios-Gonzales K, Erdei L, Lips SH (2002) The activity of antioxidant enzymes in maize and sunflower seedlings as affected by salinity and different nitrogen sources. Plant Sci 162:923–930
• Sairam RK, Rao KV, Srivastava GC (2002) Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolytes concentration. Plant Sci 163:1037–1046
• Scandalios JG, Guan L, Polidoros AN (1997) Catalases in plants: gene structure, properties, regulation, and expression. In: Scandalios JG (ed) Oxidative stress and the molecular biology of antioxidant defenses. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp 343–406
• Sergio L, Paola AD, Cantore V, Pieralice M, Cascarano NA, Bianco VV, Venere DD (2012) Effect of salt stress on growth parameters, enzymatic antioxidant system, and lipid peroxidation in wild chicory (Cichorium intybus L.). Acta Physiol Plant. doi:10.1007/s11738-012-1038-3
• Shannon MC, Grieve CM, Francois LE (1994) Whole-plant response to salinity. In: Wilkinson RE (ed) Plant-environment interactions. Marcel Dekker, Inc., New York, pp 199–244
• Shekhawat GS, Verma K, Jana S, Singh K, Teotia P, Prasad A (2010) In vitro biochemical evaluation of cadmium tolerance mechanism in callus and seedlings of Brassica juncea. Protoplasma 239:31–38
• Shigeoka S, Ishikawa T, Tamoi M, Miyagawa Y, Takeda T, Yabuta Y, Yoshimura K (2002) Regulation and function of ascorbate peroxidase isoenzymes. J Exp Bot 53:1305–1319
• Shiyab SM, Shibli RA, Mohammad MM (2003) Influence of sodium chloride salt stress on growth and nutrient acquisition of sour orange in vitro. J Plant Nutr 26:985–996
• Slama I, Ghnaya T, Savoure0 A, Abdelly C (2008) Combined effects of long-term salinity and soil drying on growth, water relations, nutrient status and proline accumulation of Sesuvium portulacastrum. C R Biol 331:442–451
• Tester M, Davenport R (2003) Na? tolerance and Na+ transport in higher plants. Ann Bot 91:503–527
• Tripathi YB, Chaurasia S, Tripathi E, Upadhyay A, Dubey GP (1996) Bacopa monniera Linn. as an antioxidant: mechanism of action. Ind J Exp Biol 34:523–526
• Troncoso A, Matte C, Cantos M, Lavee S (1999) Evaluation of salt tolerance of in vitro grown grapevine rootstock varieties. Vitis 38:55–60
• Unlukara A, Kurunc A, Kesmez GD, Yurtseven E, Suarez DL (2010) Effects of salinity on eggplant (Solanum melongena L.) growth and evapotranspiration. Irrig Drain 59:203–214
• Verslues P, Juenger T (2011) Drought, metabolites and Arabidopsis natural variation: a promising combination for understanding adaptation to water-limited environments. Curr Opin Plant Biol 14:240–245
• Vijayan K, Chakraborti SP, Ghosh PD (2003) In vitro screening of mulberry (Morus spp.) for salinity tolerance. Plant Cell Rep 22:350–357
• Wahid A, Ghazanfar A (2006) Possible involvement of some secondary metabolites in salt tolerance of sugarcane. J Plant Physiol 163:723–730
• Watanabe S, Kojima K, Ide Y, Sasaki S (2000) Effects of saline and osmotic stress on proline and sugar accumulation in Populus euphratica in vitro. Plant Cell Tiss Org Cult 63:199–206
• Watoo P, Waraporn P, Hiroyuki T, Kanchalee J, Sakchai W, Kornkanok I (2007) Comparison of various extraction methods of Bacopa monniera. Naresuan Univ J 15:29–34
• Woodward AJ, Bennett IJ (2005) The effect of salt stress and abscisic acid on proline production, chlorophyll content and growth of in vitro propagated shoots of Eucalyptus camaldulensis. Plant Cell Tiss Org Cult 82:189–200
• Yasar F, Ellialtioglu S, Kusvuran S (2006) Ion and lipid peroxide content in sensitive and tolerant eggplant callus cultured under salt stress. Eur J Hort Sci 71:S169–S172
• Zayed MA, Zeid IM (1998) Effect of water and salt stresses on growth, chlorophyll, mineral ions and organic solutes contents, and enzymes activity in mung bean seedlings. Biol Plant 40:351–356
• Zhang F, Yang YL, He WL, Zhao X, Zhang LX (2004) Effects of salinity on growth and compatible solutes of callus induced from Populus euphratica. In Vitro Cell Dev Biol Plants 40:491–494
• Zhu JK (2001) Plant salt tolerance. Trends Plant Sci 6:66–71
• Zhu JK (2002) Salt and drought stress signal transduction in plants. Ann Rev Plant Biol 53:247–273

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