PTPN4 negatively regulates CRKI in human cell lines

• Autorzy: Zhou J. , Wan B. , Shan J. , Shi H. , Li Y. , Huo K.
• Języki publikacji: EN

Abstrakty

EN

PTPN4 is a widely expressed non-receptor protein tyrosine phosphatase. Although its overexpression inhibits cell growth, the proteins with which it interacts to regulate cell growth are unknown. In this study, we identified CrkI as a PTPN4-interacting protein using a yeast two-hybrid, and confirmed this interaction using in vitro GST pull-down and co-immunoprecipitation and co-localization assays. We further determined the interactional regions as the SH3 domain of CrkI and the proline-rich region between amino acids 462 and 468 of PTPN4. Notably, overexpression of PTPN4 inhibits CrkI-mediated proliferation and wound healing of HEK293T cells, while knockdown of PTPN4 by siRNA in Hep3B cells enhances CrkI-mediated cell growth and motility. Moreover, our data show that ectopic expression of PTPN4 reduces the phosphorylation level of CrkI in HEK293T cells. These findings suggest that PTPN4 negatively regulates cell proliferation and motility through dephosphorylation of CrkI.

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2013
• Tom: 18
• Numer: 2
• Opis fizyczny: p.297-314,fig.,ref.

Twórcy

autor

Zhou J.

• State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life sciences, Fudan University, 220 Handan Rd., Shanghai 200433, China

autor

Wan B.

autor

Shan J.

autor

Shi H.

autor

Li Y.

autor

Huo K.

Bibliografia

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