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2015 | 42 |
Tytuł artykułu

RAPD-PCR as a molecular discriminative technique for human pathogenic bacteria- A Review

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Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The application of random amplified polymorphic DNA- polymerase chain reaction (RAPDPCR) was found to be a simple, cheap and rapid tool to discriminate human pathogenic bacterial isolates especially at intraspecific level. This molecular biological technique relies on the use of random oligonucleotide primers that arbitrarily amplifies specific regions of the genome which gives rise to a unique genomic fingerprint of the strains under investigations. With continued development of novel molecular-based technologies for rapid, high-throughput detection of food borne pathogenic bacteria, the future of conventional microbiological methods such as viable cell enumeration, selective isolation of bacteria on commercial media, and immunoassays seems tenuous. Approaches that enhance recovery of sub lethally injured bacteria, differentiation among species, differentiation among bacteria of interest using biochemical profiling, enumeration using impedance technology, techniques to confirm the presence of target pathogens using immunological methods, and bioluminescence applications for hygiene monitoring are of utmost need in identifying and combating the human pathogenic isolates. The aim of this study is to estimate the efficiency of RAPD-PCR technique in assessing the genetic diversity of diseases causing bacterial isolates. The use of RAPD-PCR in evaluating the genomic variability among the pathogenic strains belonging to different genus are also been discussed in the present report.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
42
Opis fizyczny
p.13-17,ref.
Twórcy
autor
  • Department of Zoology, Scottish Church College, 1 and 3 Urquhart Square, Kolkata - 700006, India
Bibliografia
  • [1] N. Botteldoorn, L. Herman, N. Rijpens, et al.: Phenotypic and Molecular Typing of Salmonella Strains Reveal Different Contamination Source in Two Commercial Pig Slaughterhouses. Applied and Environmental Microbiology 70 (2004) 5305-5314.
  • [2] R. M.Weigel, B. Z. Qiaoa, B. Teferedegneb, D. K. Suh, D. A. Barber, et al.: Comparison of Pulsed Field Gel Electrophoresis and Repetitive Sequence Polymerase Chain Reaction as Genotyping Methods for Detection of Genetic Diversity and Inferring Transmission of Salmonella. Veterinary Microbiology 100 (2004) 205-217.
  • [3] H. Y. Tsen and J. S. Lin: Analysis of Salmonella Enteritidis Strains Isolated from Food-Poisoning Cases in Taiwan by Pulsed Field Gel Electrophoresis, Plasmid Profile and Phage Typing. Journal of Applied Microbiology 91 (2001) 72-79.
  • [4] J. Garaizar, N. López-Molina, I. Laconcha, D. L. Baggesen, A. Rementeria, A. Vivanco, et al.: Suitability of PCR Fingerprinting, Infrequent-Restriction Site PCR, and Pulsed-Field Gel Electrophoresis, Combined with Computerized Gel Analysis, in Library Typing of Salmonella Enterica Serovar Enteriditis. Applied and Environmental Microbiology 66 (2000) 5273-5281.
  • [5] B. Saran, Z. C. Karahan, H. Ağirbaşli, A. Tekeli, A. M. Aksoy: Comparison of different primers used for the genotyping of Candida albicans clinical isolates by randomly amplified polymorphic DNA method. Mikrobiyol Bul 42 (2008) 645–654.
  • [6] B. Krawczyk, J. Leibner-Ciszak, A. Mielech, M. Nowak, J. Kur: PCR melting profile (PCR MP) — a new tool for differentiation of Candida albicans strains. BMC Infect. Dis .9 (2009) 177–189.
  • [7] K. L. Bacelo, K. R. da Costa, J. C. Ferreira, R. C. Candido: Biotype stability of Candida albicans isolates after culture storage determined by randomly amplified polymorphic DNA and phenotypical methods. Mycoses 53 (2010) 468–474.
  • [8] B. Gültekin, M. Eyigör, Y. Tiryaki, S. Kırdar, N. Aydın: Investigation of antifungal susceptibilities and some virulence factors of Candida strains isolated from blood cultures and genotyping by RAPD-PCR. Mikrobiyol Bul 45 (2011) 306–317.
  • [9] Y. H. Samaranayake, L. P. Samaranayake, R. S. Dassanayake, J. Y. Yau, W. K. Tsang, B. P. Cheung, Y. Shiren, S. Y. Issa, E. F. Badran, K. F. Akl, A. A Shebabi: Epidemiological characteristics of Candida species colonizing oral and rectal sites of Jordanian infants. BMC Pediatr. 11(2011) 1–6.
  • [10] J. Xu, C. M. Boyd, E. Livingston, W. Meyer, J. F. Madden, T. G. Mitchell: Species and genotypic diversities and similarities of pathogenic yeasts colonizing women. J. Clin. Microbiol. 37 (1999) 3835–3843.
  • [11] P. P. Chong, Y. L. Lee, B. C. Tan, K. P. Ng: Genetic relatedness of Candida strains isolated from women with vaginal candidiasis in Malaysia. Journal of Medical Microbiology 52 (2003) 657–666.
  • [12] T. M. Diaz-Guerra, E. Mellado, M. Cuenca-Estrella, L. Gaztelurrutia, J. I. Navarro, J. L. Tudela: Genetic Similarity among one Aspergillus flavus Strain Isolated from a Patient Who Underwent Heart Surgery and Two Environmental Strains Obtained from the Operating Room. Journal of Clinical Microbiology 38 (2000) 2419-2422.
  • [13] Y. Hara Kudo, K. Sugiyama, M. Nishibuchi, et al.: Prevalence of Pandemic Thermostable Direct Hemolysin Producing Vibrio parahaemolyticus O3:K6 in Seafood and the Coastal Environment in Japan. Applied and Environmental Microbiology 69 (2003) 3883-3891.
  • [14] N. C. Leal, S. C. da Silva, V. O. Cavalcanti, et al.: Vibrio parahaemolyticus Serovar O3:K6 Gastroenteritis in Northeast Brazil. Journal of Applied Microbiology 105 (2008) 691-697.
Typ dokumentu
Bibliografia
Identyfikator YADDA
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