Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2012 | 15 | 2 |
Tytuł artykułu

Effect of infection of turkeys with haemorrhagic enteritis adenovirus isolate on the selected parameters of cellular immunity and the course of colibacillosis

Treść / Zawartość
Warianty tytułu
Języki publikacji
The aim of the study was to determine the effect of a Polish low-virulence isolate of haemorrhagic enteritis adenovirus (HEV) on the immune system in turkeys and on the course of colibacillosis in birds infected under laboratory conditions. Turkeys were infected per os with HEV at the dose of 104.3EID50/mL and with E. coli (APEC) (serotypes 078:K80:H9) at the dose of 4×109CFU/mL by injection to the thoracic air sac. The birds infected with the HEV were infected with the APEC either simultaneously or after 5 days. Five days after HEV infection, the percentages of subpopulations of the CD3+CD4+ and CD3+CD8α+ T cells and the IgM+ B cells were determined in blood and spleens of the HEV-infected turkeys and in the control (uninfected) birds. The course of colibacillosis was more severe in turkeys infected with the APEC 5 days after infection with the HEV than in those infected with the HEV and APEC simultaneously and than in those infected only with APEC. Five turkeys out of the 18 infected with the APEC 5 days after infection with HEV, died. Their body weights were statistically significantly lower with higher FCR values 41 days after the infection in comparison to turkeys in the other groups. A considerable decrease in the percentage of the T and B cells subpopulations in the blood were found in turkeys infected with the HEV and while the percentage of CD3+CD4+ T cells subpopulation in the spleen increased significantly, the contribution of the CD3+CD8α+ T cells and IgM+ B cells subpopulations were decreased. These changes in the immune system of turkeys, occurring 5 days after infection with the HEV, made them more susceptible to infection with the APEC.
Słowa kluczowe
Opis fizyczny
  • Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-718 Olsztyn, Poland
  • Barnes HJ, Vaillancourt J-P, Gross WB (2003) Collibacillosis. 11th ed In: Saif YM, Barnes HJ, Glisson JR, Fadly AM, McDougald LR, Swayne DE (eds) Disease of Poultry. Ames, Iowa State Press, pp 631-656.
  • Göbel TW, Fluri M (1997) Identification and analysis of the chicken CD3 epsilon gene. Eur J Immunol 27: 194-198.
  • Guiro S, Koncicki A (2004) Vaccination against NDV in turkeys infected by haemorrhagic enteritis virus. Med Weter 60: 871-873.
  • Hess M, Raue R, Hafez HM (1999) PCR for specific detection of haemorrhagic enteritis virus of turkeys, an avian adenovirus. J Virol Methods 81: 199-203.
  • Koncicki A (1990) First incidences of adenoviral haemorrhagic enteritis in turkeys in Poland. Med Weter 46: 16-17.
  • Koncicki A (1996) Characteristics of domestic adenovirus isolates of hemorrhagic enteritis (HE) in turkeys and evaluation of epizootic situation in Poland. Acta Acad Agricult Tech Olst Veterinaria 22: 1-43.
  • Koncicki A, Jankowski J, Zduńczyk Z, Mazur-Gonkowska B, Krasnodębska-Depta A (2004) Effect of mannano-oligosacharides on the course of turkey infection with HEV and E. coli. Med Weter 60: 968-971.
  • Kothlow S, Mannes NK, Schaerer B, Rebeski DE, Kaspers B, Schultz U (2005) Characterization of duck leucocytes by monoclonal antibodies. Dev Comp Immunol 29: 733-748.
  • Krasnodębska-Depta A, Koncicki A, Mazur-Gonkowska B (2003) Haematological and biochemical indices in the serum of turkeys infected with a pathogenic strain of E.coli. Med Weter 59: 623-625.
  • Li Z, Nestor KE, Saif YM, Fan Z, Luhtala M, Vainio O (1999) Cross-reactive anti-chicken CD4 and CD8 monoclonal antibodies suggest polymorphism of the turkey CD8alpha molecule. Poult Sci 78: 1526-1531.
  • Li Z, Nestor KE, Saif YM, Luhtala M (2000) Flow cytometric analysis of T lymphocyte subpopulations in large-bodied turkey lines and a randombred control population. Poult Sci 79: 219-223.
  • Mazur-Lech B, Koncicki A, Janta M (2009) Investigations on using a polymerase chain reaction (PCR) to detect and evaluate the distribution of haemorrhagic enteritis virus in turkeys. Med Weter 65: 413-415.
  • Newberry LA, Skeeles JK, Kreider DL, Beasley JN, Story JD, McNew RW, Berridge BR (1993) Use of virulent hemorrhagic enteritis virus for the induction of colibacillosis in turkeys. Avian Dis 37: 1-5.
  • Pierson FW, Larsen CT, Domermuth CH (1996) The production of colibacillosis in turkeys following sequential exposure to Newcastle disease virus or Bordetella avium, avirulent hemorrhagic enteritis virus, and Escherichia coli. Avian Dis 40: 837-840.
  • Powell F, Lawson M, Rothwell L, Kaiser P (2009) Development of reagents to study the turkey’s immune response: identification and molecular cloning of turkey CD4, CD8alpha and CD28. Dev Comp Immunol 33: 540-546.
  • Saunders GK, Pierson FW, Hurk JV (1993) Haemorrhagic enteritis virus infection in turkeys: a comparison of virulent and avirulent virus infections, and a proposed pathogenesis. Avian Pathol 22: 47-58.
  • Sponenberg DP, Domermuth CH, Larsen CT (1985) Field outbreaks of colibacillosis of turkeys associated with hemorrhagic enteritis virus. Avian Dis 29: 838-842.
  • Suresh M, Sharma JM (1995) Hemorrhagic enteritis virus induced changes in the lymphocyte subpopulations in turkeys and the effect of experimental immunodeficiency on viral pathogenesis. Vet Immunol Immunopathol 45: 139-150.
  • Suresh M, Sharma JM (1996) Pathogenesis of type II avian adenovirus infection in turkeys: in vivo immune cell tropism and tissue distribution of the virus. J Virol 70: 30-36.
  • Van den Hurk J, Allan BJ, Riddell C, Watts T, Potter AA (1994) Effect of infection with hemorrhagic enteritis virus on susceptibility of turkeys to Escherichia coli. Avian Dis 38: 708-716.
  • Van Nerom A, Ducatelle R, Haesebrouck F, Arnouts S, Goddeeris B, Davison TF, Kaspers B (1997) Monoclonal and polyclonal antibodies to chicken immunoglobulin isotypes specifically detect turkey immunoglobulin isotypes. Vet Immunol Immunopathol 57: 305-314.
  • Yogaratnam V (1995) Analysis of the causes of high rates of carcase rejection at a poultry processing plant. Vet Rec 137: 215-217
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.