PCR-RFLP method to distinguish Frankliniella occidentalis, Frankliniella intonsa, Frankliniella pallida and Frankliniella tenuicornis

• Autorzy: Przybylska A. , Fiedler Z. , Obrepalska-Steplowska A.
• Języki publikacji: EN

Abstrakty

EN

Thrips from the genus Frankliniella (Thysanoptera, Thripidae) are phytophagous on crops and wild plants. Some of them cause slight economic damage, however, others including F. occidentalis and F. intonsa are responsible for considerable losses in crop production. Moreover, they constitute a double threat for host plants by not only feeding on them but also vectoring viruses, some of which are on the quarantined list of the European Plant Protection Organization. The rapid detection and differentiation between more and less harmful Frankliniella species is, therefore, important in order to combat the pests at the time of their appearance. In this study, we have undertaken to develop a method of detecting F. occidentalis, F. intonsa, F. pallida, and F. tenuicornis. The protocol is based on PCR amplification of ITS1 rDNA fragments of these insects using universal primers pair giving products of slightly distinct length for studied insects. Restriction enzymes digestion which is easy to interpret, allows for visible differentiation of all these Frankliniella species. The method was shown to be species-specific and sensitive. Even single specimens in either the larvae or adult stage could be distinguished.

Słowa kluczowe

EN

PCR-RFLP method; Frankliniella occidentalis; Frankliniella intonsa; Frankliniella pallida; Frankliniella tenuicornis; thrip; molecular diagnostics; pest control; detection

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2016
• Tom: 56
• Numer: 1
• Opis fizyczny: p.60-66,fig.,ref.

Twórcy

autor

Przybylska A.

• Interdepartmental Laboratory of Molecular Biology, Institute of Plant Protection - National Research Institute, Wegorka 20, 60-318 Poznan, Poland

autor

Fiedler Z.

• Department of Biological Pest Control, Institute of Plant Protection - National Research Institute, Wegorka 20, 60-318 Poznan, Poland

autor

Obrepalska-Steplowska A.

• Interdepartmental Laboratory of Molecular Biology, Institute of Plant Protection - National Research Institute, Wegorka 20, 60-318 Poznan, Poland

Bibliografia

• Brunner P., Fleming C., Frey J. 2002. A molecular identification key for economically important thrips species (Thysanoptera: Thripidae) using direct sequencing and a PCR-RFLP-based approach. Agricultural and Forest Entomology 4 (2): 127–136.
• EPPO A. List of pests recommended for regulation as quarantine pests. EPPO (version 2014-09). Available on: http://www.ppo.org/QUARANTINE/listA2.html. [Accessed: August 28, 2015]
• Hall T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
• Huang C.-G., Hsu J.-C., Haymer D.S., Lin G.-C., Wu W.-J. 2009. Rapid identification of the Mediterranean fruit fly (Diptera: Tephritidae) by loop-mediated isothermal amplification. Journal of Economic Entomology 102 (3): 1239–1246.
• Huang K., Lee S., Yeh Y., Shen G., Mei E., Chang C. 2010. Taqman real-time quantitative PCR for identification of western flower thrip (Frankliniella occidentalis) for plant quarantine. Biology Letters 6 (4): 555–557.
• Kakkar G., Seal D.R., Jha V.K. 2010. Common blossom thrips, Frankliniella schultzei Trybom (Insecta: Thysanoptera: Thripidae). Available on: http://edis.ifas.ufl.edu/pdffiles/IN/IN86000.pdf [Accessed: August 28, 2015]
• Kraus M.S.G., Moritz G. 1998. Molecular genetic studies of thrips species. p. 77–80. In: Proceedings of the 6th International Symposium on Thysanoptera, Akdeniz University, Antalya, Turkey, 27 April–1 May 1998.
• Kucharczyk H. 2007. Wciornastki. [“Thrips“]. p. 391–398. In: "Fauna Polski: charakterystyka i wykaz gatunków” [“Fauna of Poland. Characteristics and Checklist of Species”]. Vol. 2. (W. Bogdanowicz, E. Chudzicka, I. Pilipiuk, E. Skibińska, eds.), Muzeum i Instytut Zoologii PAN, Warszawa, 509 pp.
• Mainali B.P., Shrestha S., Lim U.T., Kim Y. 2008. Molecular markers of two sympatric species of the genus Frankliniella (Thysanoptera: Thripidae). Journal of Asia-Pacific Entomology 11 (1): 45–48.
• Marullo R. 2002. Impact of an introduced pest thrips on the indigenous natural history and agricultural systems of southern Italy. p. 285–288. In: “Thrips and Tospoviruses”. Proceedings of the 7th International Symposium on Thysanoptera, Australian National Insect Collection, Reggio Calabria, Italy, 02–07 July 2001.
• Marullo R. 2003. Conoscere i Tisanotteri. Guide al riconoscimento delle specie dannose alle colture agrarie. Edagricole, Bologne, Italy, 75 pp.
• Moritz G., Mound L., Morris D., Goldgrazena A. 2004. Pest thrips of the world. An identification and information system using molecular and microscopical methods. CD ROM, University of Qeensland, Brisbane.
• Moritz G.D.C., Paulsen M., Mound L.A., Burgermeister W. 2000. Modern methods for identification of Thysanoptera. EPPO Bulletin 30: 591–593.
• Nakahara S. 1997. Annotated list of the Frankliniella species of the world (Thysanoptera: Thripidae). Contributions on Entomology, International 2 (4): 355–389.
• Nicholas K.B., Nicholas Jr H.B., Deerfield II D.W. 1997. Gene Doc: analysis and visualization of genetic variation. Embnet News 4: 1–4.
• Obrepalska-Steplowska A., Nowaczyk K., Holysz M., Gawlak M., Nawrot J. 2008. Molecular techniques for the detection of granary weevil (Sitophilus granarius L.) in wheat and flour. Food Additives and Contaminants 25 (10): 1179–1188.
• Pobożniak M. 2011. Dynamika populacji Frankliniella intonsa (Thysanoptera) na warzywach bobowatych. [Population dynamic of Frankliniella intonsa (Thysanoptera) on legumes vegetables]. Progress in Plant Protection 51 (3): 1058–1062.
• Przybylska A., Fiedler Ż., Kucharczyk H., Obrępalska-Stęplowska A. 2015. Detection of the quarantine species Thrips palmi by loop-mediated isothermal amplification. PloS ONE 10 (3): e0122033.
• Pumariño L., Alomar O., Agustí N. 2011. Development of specific ITS markers for plant DNA identification within herbivorous insects. Bulletin of Entomological Research 101 (3): 271–276.
• Rebijith K., Asokan R., Kumar N.K., Krishna V., Ramamurthy V. 2012. Development of species-specific markers and molecular differences in mtDNA of Thrips palmi Karny and Scirtothrips dorsalis Hood (Thripidae: Thysanoptera), vectors of tospoviruses (Bunyaviridae) in India. Entomological News 122 (3): 201–213.
• Sierka W., Gocyła A. 2004. A survey and geographical distribution of the thrips (Insecta, Thysanoptera) in Poland. Thysanopteron 1: 24–57.
• Toda S., Komazaki S. 2002. Identification of thrips species (Thysanoptera: Thripidae) on Japanese fruit trees by polymerase chain reaction and restriction fragment length polymorphism of the ribosomal ITS2 region. Bulletin of Entomological Research 92 (4): 359–363.
• Ullah M.S., Lim U.T. 2015. Life history characteristics of Frankliniella occidentalis and Frankliniella intonsa (Thysanoptera: Thripidae) in constant and fluctuating temperatures. Journal of Economic Entomology 108 (3): 1000–1009.
• Varadínová Z., Wang Y., Kučerová Z., Stejskal V., Opit G., Cao Y., Li F., Li Z. 2015. COI barcode based species-specific primers for identification of five species of stored-product pests from genus Cryptolestes (Coleoptera: Laemophloeidae). Bulletin of Entomological Research 105 (2): 202–209.
• Wang C.-L., Lin F.-C., Chiu Y.-C., Shih H.-T. 2010. Species of Frankliniella Trybom (Thysanoptera: Thripidae) from the Asian-Pacific Area. Zoological Stuies 49 (6): 824–848.
• Zhang G., Meng X., Min L., Qiao W., Wan F. 2012. Rapid diagnosis of the invasive species, Frankliniella occidentalis (Pergande): a species – specific COI marker. Journal of Applied Entomology 136 (6): 410–420.

Identyfikatory

DOI

10.1515/jppr-2016-0009