Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 36 | 10 |
Tytuł artykułu

Analysis of emergence stage facilitates the evaluation of chickpea (Cicer arietinum L.) genotypes for salinity tolerance imparted by mycorrhizal colonization

Treść / Zawartość
Warianty tytułu
Języki publikacji
Salinity is an ever-increasing constraint limiting crop production in arid and semi-arid regions. Arbuscular mycorrhiza (AM) helps host plant to cope with detrimental effects of salinity. Experiments were aimed to examine the hypothesis that emergence is a better stage to determine salt tolerance of chickpea genotypes than germination and genotypic variability in their tolerance ability at emergence and subsequent vegetative growth is the manifestation of differential benefits imparted by mycorrhiza. Investigations were carried out at germination and emergence stage of genotypes (PBG 5, GPF 2, PBG 1, BG 1053, L 550) at 0, 40, 60, 80 mM NaCl. Significant genotypic variations in salt tolerance were observed at emergence rather than germination because of greater inhibitory effects on seedling emergence. Percent mycorrhizal colonization (MC) and its resulting impact on respiration rate (RR) and salt tolerance index (STI) at emergence indicated that PBG 5, with lowest RR, highest STI and mycorrhiza benefit percentage was the most tolerant whereas, L 550 the most sensitive genotype. Genotypic variability recorded at 30 days was consistent with that at emergence stage. Superior salt tolerance of PBG 5 than L 550 could be attributed to higher correlation between MC and physio-biochemical traits (RWC, chlorophyll a/b, proline accumulation, antioxidant activities). The study supported the hypothesis that both emergence stage and mycorrhizal effectiveness are important determinants of salt tolerance in chickpea genotypes. Evaluation of genotypes for relative adaptation to salinity should include estimation of their differential salt tolerance at different growth stages and symbiotic effectiveness of AM.
Słowa kluczowe
Opis fizyczny
  • Department of Botany, Panjab University, Chandigarh, 160014, India
  • Department of Botany, Panjab University, Chandigarh, 160014, India
  • Department of Botany, Panjab University, Chandigarh, 160014, India
  • Abbott LK, Robson AD (1981) Infectivity and effectiveness of vesicular–arbuscular fungi: effect of inoculums type. Aust J Agric Res 32:631–639
  • Aebi H (1984) Catalase in vitro. In: Packer L (ed) Methods in enzymology. Academic press, Orlando, 105, pp 121–126
  • Al–Karaki GN, Hammad R, Rusan M (2001) Response of two tomato cultivars differing in salt tolerance to inoculation with mycorrhizal fungi under salt stress. Mycorrhiza 11:41–47
  • Al–Khaliel AS (2010) Effect of salinity stress on mycorrhizal association and growth response of peanut infected by Glomus mosseae. Plant Soil Env 56:318–324
  • Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenyloxidase in Beta vulgaris. Plant Physiol 24:1–15
  • Aroca R, Bago A, Sutka M, Paz JA, Cano C, Amodeo G, Ruiz-Lozano JM (2009) Expression analysis of the first arbuscular mycorrhizal fungi aquaporin described reveals concerted gene expression between salt–stressed and nonstressed mycelium. Mol Plant Microbe Int 22:1169–1178
  • Ashraf M, Foolad MR (2005) Pre–sowing seed treatment–a shotgun approach to improve germination growth and crop yield under saline and none–saline conditions. Advan Agron 88:223–271
  • Ashraf M, Harris P (2004) Potential biochemical indicators of salinity tolerance in plants. Plant Sci 166:3–16
  • Aydemir T, Erez Z (2010) Physiological and biochemical response to NaCl stress in Lens culinaris. CBU J Sci 6:89–104
  • Barloy J (1984) Phase germination levée et implantation. In: Gallais A (ed) Physiologie du Maïs. INRA, CNRS, AGPM, Paris, pp 13–48
  • Bates LS, Waldran RP, Teare ID (1973) Rapid determination of free proline for water studies. Plant Soil 39:205–208
  • Beltrano J, Ruscitti M, Arango MC, Ronco M (2013) Effects of arbuscular mycorrhiza inoculation on plant growth, biological and physiological parameters and mineral nutrition in pepper grown under different salinity and P levels. J Soil Sci Plant Nutr 13:123–141
  • Boughanmi N, Michonneau P, Daghfous D, Fleurat-Lessard P (2005) Adaptation of Medicago sativa cv. Gabes to long–term NaCl stress. J Plant Nutr Soil Sci 168:262–268
  • Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye-binding. Anal Biochem 72:248–254
  • Cantrell IC, Linderman RG (2001) Preinoculation of lettuce and onion with VA mycorrhizal fungi reduces deleterious effects of soil salinity. Plant Soil 233:269–281
  • Castillo FI, Penel I, Greppin H (1984) Peroxidase release induced by ozone in Sedum album leaves. Plant Physiol 74:846–851
  • Chapman HD, Pratt PF (1961) Methods of analysis for soil, plant and waters, first ed. Division of Agricultural Sciences, University of California, Riverside, Berkley, USA, pp 150 - 210
  • Colla G, Rouphael Y, Cardarelli M, Tullio M, Rivera CM, Rea E (2008) Alleviation of salt stress by arbuscular mycorrhizal in zucchini plants grown at low and high phosphorus concentration. Biol Fert Soils 44:501–509
  • Dankov AK, Mira B, Detelin S, Emilia LA (2009) Relationship between the degree of carotenoid depletion and function of the photosynthetic apparatus. J Photochem Photobiol B Biol 96:49–56
  • Dhindsa RS, Plumb-Dhindsa P, Throne TA (1981) Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation and decreased levels of superoxide dismutase and catalase. J Exp Bot 32:93–101
  • Dubey RS (1997) Photosynthesis in plants under stressful conditions. In: Pessarakli M (ed) Handbook of Photosynthesis. Marcel Dekker, New York, pp 859–875
  • Dudhane MP, Borde MY, Jite PK (2011) Effect of arbuscular mycorrhizal fungi on growth and antioxidant activity in Gmelina arborea Roxb. under salt stress condition. Not Sci Biol 3:71–78
  • El Naim AM, Mohammed KE, Ibrahim EA, Suleiman NN (2012) Impact of salinity on seed germination and early seedling growth of three sorghum (Sorghum biolor L. Moench) cultivars. Sci Tech 2:16–20
  • El–Hendawy SE, Hu Y, Sakagami JI, Schmidhalter U (2005) Growth, ion content, gas exchange and water relations of wheat genotypes differing in salt tolerances. Aust J Agric Res 56:123–134
  • Esechie HA, Al–Saidi A, Al–Khanjari S (2002) Effect of sodium chloride salinity on seedling emergence in chickpea. J Agron Crop Sci 188:155–160
  • Evelin H, Kapoor R, Giri B (2009) Arbuscular mycorrhizal fungi in alleviation of salt stress: a review. Ann Bot 104:1263–1280
  • FAO (2011) The state of the world’s land and water resources for food and agriculture (SOLAW) – Managing systems at risk. Food and Agriculture Organization of the United Nations, Rome and Earthscan, London
  • FAOSTAT (2010) FAO (Food and Agricultural Organization of the United Nations). Rome, Italy.
  • Feng G, Zhang FS, Li XI, Tian CY, Tang C, Rengel Z (2002) Improved tolerance of maize plants to salt stress by arbuscular mycorrhiza is related to higher accumulation of soluble sugars in roots. Mycorrhiza 12:185–190
  • Flowers TJ, Galal HK, Bromham L (2010) Evolution of halophytes: multiple origins of salt tolerance in land plants. Funct Plant Biol 37:604–612
  • Garg N, Baher N (2013) Role of arbuscular mycorrhizal symbiosis in proline biosynthesis and metabolism of Cicer arietinum L. (Chickpea) genotypes under salt stress. J Plant Growth Regul 32:767–778
  • Geol N, Varshney KA (1987) Note on seed germination and early seedling growth of two chickpea varieties under saline conditions. Legume Res 10:34–36
  • Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930
  • Giri B, Kapoor R, Mukerji KG (2007) Improved tolerance of Acacia nilotica to salt stress by arbuscular mycorrhiza, Glomus fasciculatum, may be partly related to elevated K⁺/Na⁺ ratios in root and shoot tissues. Microb Ecol 54:753–760
  • Grigore MN, Boscaiu M, Vicente O (2011) Assessment of the relevance of osmolyte biosynthesis for salt tolerance of halophytes under natural conditions. Eur J Plant Sci Biotech 5:12–19
  • Hajiboland R, Aliasgharzadeh A, Laiegh SF, Poschenrieder C (2010) Colonization with arbuscular mycorrhizal fungi improves salinity tolerance of tomato (Solanum lycopersicum L.) plants. Plant Soil 331:313–327
  • Heath RL, Packer I (1968) Photoperoxidation in isolated chloroplast I, Kinetics and stochiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
  • Hiscox TD, Israelstam GF (1979) A method for extraction of chlorophyll from leaf tissue without maceration. Can J Bot 57:1332–1334
  • Hossain MA,Mostofa MG,Fujita M (2013) Crossprotectionby cold–shock to salinity and drought stress–induced oxidative stress in mustard (Brassica campestris L.) seedlings. Mol Plant Breeding 4:50–70
  • Javot H, Maurel C (2002) The role of aquaporins in root water uptake. Ann Bot 90:301–313
  • Juniper S, Abbott L (1993) Vesicular and arbuscular mycorrhizae and soil salinity. Mycorrhiza 4:45–57
  • Juniper S, Abbott LK (2006) Soil salinity delays germination and limits growth of hyphae from propagules of arbuscular mycorrhizal fungi. Mycorrhiza 16:371–379
  • Kapoor R, Sharma D, Bhatnagar AK (2008) Arbuscular mycorrhizae in micropropogation systems and their potential applications. Sci Hort 116:227–239
  • Karlidag H, Yildirim E, Turan M (2011) Role of 24–epibrassinolide in mitigating the adverse effects of salt stress on stomatal conductance, membrane permeability, and leaf water content, ionic composition in salt stressed strawberry (Fragaria × ananassa). Sci Hortic 130:133–140
  • Kavi Kishor PB, Sreenivasulu N (2014) Is proline accumulation per se correlated with stress tolerance or is proline homeostasis a more critical issue? Plant Cell Environ 37:300–311
  • Khajeh-Hosseini M, Powell AA, Bingham IJ (2003) The interaction between salinity stress and seed vigour during germination of soybean seeds. Seed Sci Technol 31:715–725
  • Koshal AK (2012) Satellite image analysis of salinity areas through GPS, Remote sensing and GIS. In: 14th Annual International Conference and Exhibition on Geospatial Technology and Applications, India Geospatial Forum
  • Kumar A, Mangla C, Aggarwal A, Parkash V (2010) Arbuscular mycorrhizal fungal dynamics in the rhizospheric soil of five medicinal plant species. Middle-East J Scientific Res 6:281–288
  • Lambais MR, Rios-Ruiz WF, Andrade RM (2003) Antioxidant responses in bean (Phaseolus vulgaris) roots colonized by arbuscular mycorrhizal fungi. New Phytol 160:421–428
  • Läuchli A, Grattan SR (2007) Plant growth and development under salinity stress. In: Jenks MA, Hasegawa PM, Jain SM (eds) Advances in molecular breeding toward drought and salt tolerant crops. Springer, The Netherlands, pp 1–32
  • Li Y (2008) Effect of salt stress on seed germination and seedling growth of three salinity plants. Pak J Biol Sci 11:1268–1272
  • Li T, Hu Y-J, Hao Z-P, Li H, Wang Y-S, Chen B-D (2013) First cloning and characterization of two functional aquaporin genes from an arbuscular mycorrhizal fungus Glomus Intraradices. New Phytol 197:617–630
  • Liu RJ, Luo XS (1994) A new method to quantify the inoculum potential of arbuscular mycorrhizal fungi. New Phytol 128:89–92
  • Lopez-Climent M, Arbona V, Perez-Clemente RM, Gomez-Cadenas A (2008) Relationship between salt tolerance and photosynthetic machinery performance in citrus. Environ Exp Bot 62:176–184
  • Maathuis FJM (2006) The role of monovalent cation transporters in plant responses to salinity. J Exp Bot 57:1137–1147
  • Mahajan S, Tuteja N (2005) Cold, salinity and drought stresses: an overview. Arch Biochem Biophy 444:139–158
  • McGonigle TP, Miller MH, Evans DG, Fairchild GL, Swan JA (1990) A new method which gives an objective measure of colonization of roots by vesicular–arbuscular mycorrhizal fungi. New Phytol 115:495–501
  • Mehlich A (1953) Determination of P, Ca, Mg, K, Na and NH₄. In: Short test methods used in Soil Testing Division, Department of Agriculture, Raleigh, North Carolina
  • Miyamoto S, Piela K, Patticrew J (1985) Salt effects on germination and seedling emergence of several vegetable crops and guayule. Irrig Sci 6:159–170
  • Miyamoto S, Foster M, Trostle C, Glenn E (2012) Salt tolerance of oilseed crops during establishment. J Arid Land Studies 22:147–151
  • Moud AM, Maghsoudi K (2008) Salt stress effects on respiration and growth of germinated seeds of different wheat (Triticum aestivum L.) cultivars. World J Agric Sci 4:351–358
  • Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annu Rev Plant Biol 59:651–681
  • Munns R, James RA, Läuchli A (2006) Approaches to increasing the salt tolerance of wheat and other cereals. J Exp Bot 57:1025–1043
  • Namdari A, Baghbani A (2013) Seed priming with salicylic acid induces salinity tolerance in Smooth Vetch (Vicia dasycarpa) seedling by enhanced antioxidant activities. Int J Agron Plant Produc 4:1798–1805
  • Naser L, Kourosh V, Bahman K, Reza A (2010) Soluble sugars and proline accumulation play a role as effective indices for drought tolerance screening in Persian walnut (Juglans regia L.) during germination. Fruits 65:97–112
  • Nelson DW, Sommers LE (1973) Determination of total nitrogen in plant material. Agron J 65:109–112
  • Olsen SR, Sommers LE (1982) Phosphorus. In: Page AL (ed) Methods of soil analysis, Agron. No. 9, Part 2-Chemical and microbiological properties, 2nd edn. American Society of Agronomy, Madison, Wisconsin, USA, pp 403–430
  • Pandey N, Archana N (2013) Antioxidant responses and water status in Brassica seedlings subjected to boron stress. Acta Physiol Plant 35:697–706
  • Parvaiz A, Satyawati S (2008) Salt stress and phyto biochemical responses of plants. Plant Soil Environ 54:89–99
  • Phillips JM, Hayman DS (1970) Improved procedures for clearing and staining parasitic and vesicular–arbuscular mycorrhizal fungi for rapid assessment of infection. T Brit Mycol Soc 55:158–161
  • Porcel R, Barea JM, Ruiz-Lozano JM (2003) Antioxidant activities in mycorrhizal soybean plants under drought stress and their possible relationship to the process of nodule senescence. New Phytol 157:135–143
  • Rabie GH, Almadini AM (2005) Role of bioinoculants in development of salt–tolerance of Vicia faba plants under salinity stress. Afr J Biotechnol 4:210–222
  • Raju PS, Clark RB, Ellis JR, Duncan RR, Maranville JW (1990) Benefit and cost analysis and phosphorus mycorrhizal fungi colonizations with sorghum (Sorghum bicolor) genotypes grown at varied phosphorus levels. Plant Soil 124:199–204
  • Ruan CJ, da Silva JAT, Mopper S, Qin P, Lutts S (2010) Halophyte improvement for a salinized world. Crit Rev Plant Sci 29:329–359
  • Ruiz-Lozano JM (2003) Arbuscular mycorrhizal symbiosis and alleviation of osmotic stress: new perspectives for molecular studies. Mycorrhiza 13:309–317
  • Ruiz-Lozano JM, Porcel R, Azcón C, Aroca R (2012) Regulation by arbuscular mycorrhizae of the integrated physiological response to salinity in plants: new challenges in physiological and molecular studies. J Exp Bot 63:4033–4044
  • Ruth B, Khalvati M, Schmidhalter U (2011) Quantification of mycorrhizal water uptake via high–resolution on–line water content sensors. Plant Soil 342:459–468
  • Sade N, Gebretsadik M, Seligmann R, Schwartz A, Wallach R, Moshelion M (2010) The role of tobacco aquaporin1 in improving water use efficiency, hydraulic conductivity, and yield production under salt stress. Plant Physiol 152:245–254
  • Saha P, Kunda P, Biswas AK (2012) Influence of sodium chloride on the regulation of Krebs cycle intermediates and enzymes of respiratory chain in mungbean (Vigna radiata L. Wilczek) seedlings. Plant Physiol Biochem 60:214–222
  • Samineni S, Siddique KHM, Gaur PM, Colmer TD (2011) Salt sensitivity of the vegetative and reproductive stages in chickpea (Cicer arietinum L.): podding is a particularly sensitive stage. Env Exp Bot 71:260–268
  • Shabala S (2013) Learning from halophytes: physiological basis and strategies to improve abiotic stress tolerance in crops. Ann Bot. doi:10.1093/aob/mct205
  • Shabala L, Mackay A, Tian Y, Jacobsen SE, Zhou DW, Shabala S (2012) Oxidative stress protection and stomatal patterning as components of salinity tolerance mechanism in quinoa (Chenopodium quinoa). Physiol Plant 146:26–38
  • Sharifi M, Ghorbanli M, Ebrahimzadeh H (2007) Improved growth of salinity–stressed soybean after inoculation with pre–treated mycorrhizal fungi. J Plant Physiol 164:1144–1151
  • Sharma P, Jha AB, Dubey RS, Pessarakli M (2012) Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Hindawi Publishing Corporation. J. Bot Volume 2012:26. doi:10.1155/2012/217037
  • Sharma P, Sardana V, Banga SS (2013) Salt tolerance of Indian mustard (Brassica juncea) at germination and early seedling growth. Environ Exp Biol 11:39–46
  • Sheng M, Tang M, Chan H, Yang B, Zhang F, Huang Y (2008) Influence of arbuscular mycorrhizae on photosynthesis and water status of maize plants under salt stress. Mycorrhiza 18:287–296
  • Smith SE, Facelli E, Pope S, Smith FA (2010) Plant performance in stressful environments: interpreting new and established knowledge of the roles of arbuscular mycorrhiza. Plant Soil 326:3–20
  • Thiam M, Champion A, Diouf D, Mame Ourèye SY (2013) NaCl effects on in vitro germination and growth of some senegalese cowpea (Vigna unguiculata (L.) Walp.) cultivars. Hindawi Publishing Corporation. ISRN Biotech 2013:11
  • Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidative systems in acid rain treated bean plants. Plant Sci 151:59–66
  • Vysotskaya L, Hedley PE, Sharipova G, Veselov D, Kudoyarova G, Morris J, Jones HG (2010) Effect of salinity on water relations of wild barley plants differing in salt tolerance. AoB PLANTS. doi:10.1093/aobpla/plq006
  • Walkley A (1947) A critical examination of a rapid method for determining organic carbon in soils: effects of variations in digestion conditions and of organic soil constituents. Soil Sci 63:251–264
  • Weatherley PE (1950) Studies in the water relations of cotton plant. I. The field measurement of water deficits in leaves. New Phytol 49:81–97
  • Wu QS, Zou YN, Liu W, Ye XF, Zai HF, Zhao LJ (2010) Alleviation of salt stress in citrus seedlings inoculated with mycorrhiza: changes in leaf antioxidant defense systems. Plant Soil Env 56:470–475
  • Wu C, Wang Q, Xie B, Wang Z, Cui J, Hu T (2011) Effects of drought and salt stress on seed germination of three leguminous species. Afr J Biotechnol 10:17954–17961
  • Yadav HD, Yadav OP, Dhankar OP, Oswal MC (1989) Effect of chloride salinity and boron on germination, growth, and mineral composition of chickpea (Cicer arietinum L.). Ann Arid Zone 28:63–67
  • Yildirim E, Turan M, Guvenc I (2008) Effect of foliar salicylic acid applications on growth, chlorophyll and mineral content of cucumber (Cucumis sativus L.) grown under salt stress. J Plant Nutr 31:593–612
  • Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:247–273
  • Zwiazek JJ, Blake TJ (1991) Early detection of membrane injury in black spruce (Picea mariana). Can J For Res 21:401–404
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.