Specificity of mass spectrometry (MALDI-TOF) in the diagnosis of Babesia canis regarding to other canine vector-borne diseases

• Autorzy: Dziegiel B. , Adaszek L. , Banach T. , Winiarczyk S.
• Języki publikacji: EN

Abstrakty

EN

The canine vector-borne diseases (CVBD) is a term, which describes a range of infectious or/and parasitic diseases whose etiological agents are transmitted by vectors. CVBD are becoming more widely in the world in relation to global warming and the increasing number of infected vectors. The aim of this study was to assess rapid mass spectrometry (MS) – based proteomics analyses for diagnosis of Babesia canis, Anaplasma phagocytophilum and Borrelia burgdorferi infections in dogs. The study was conducted on four groups of dogs – healthy dogs (group 1, n=10) and dogs infected with B. canis (group 2, n=20), A. phagocytophilum (group 3, n=20) and B. burgdorferi (group 4, n=20) which demonstrated symptoms of the diseases. The MALDI-TOF (Matrix Assisted Laser Desorption Ionization with Time of Flight detector) MS technique revealed the presence of specific protein fraction of 51–52 kDa only in the blood serum of all the animals infected with the B. canis protozoa. The proteins are suspected to be disease markers, whereas the MALDI-TOF technique itself has high specificity and sensitivity and can be applied in the diagnosis of canine babesiosis.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2016
• Tom: 62
• Numer: 2
• Opis fizyczny: p.101–105,fig.,ref.

Twórcy

autor

Dziegiel B.

• Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland

autor

Adaszek L.

• Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland

autor

Banach T.

• Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland

autor

Winiarczyk S.

• Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland

Bibliografia

• [1] Otranto D., Dantas-Torres F., Breitschwerdt E.B. 2009. Managing canine vector-borne diseases of zoonotic concern: part one. Trends in Parasitology 25:157-163.
• [2] Otranto D., Dantas-Torres F., Breitschwerdt E.B. 2009. Managing canine vector-borne diseases of zoonotic concern: part two. Trends in Parasitology 25:228-235.
• [3] Day M.J. 2011. The immunopathology of canine vector-borne diseases. Parasites and Vectors 4:48.
• [4] Billeter S.A, Levy M.G., Chomel B.B., Breitschwerdt E.B. 2008. Vector transmission of Bartonella species with emphasis on the potential for tick transmission. Medical and Veterinary Entomology 22:1-15.
• [5] Perez C., Maggi R.G., Diniz P.P., Breitschwerdt E.B. 2011. Molecular and serological diagnosis of Bartonella infection in 61 dogs from the United States. Journal of Veterinary Internal Medicine 25:805-810.
• [6] Adaszek Ł., Winiarczyk S. 2010. Application of the SYBR Green real-time HRM PCR technique in the differentiation of the Babesia canis canis protozoa isolated in areas of eastern Poland. Parasitology Research 106:1253-1256.
• [7] Zahler M., Schein E., Rinder H., Gothe R. 1998. Characteristic genotypes discriminate between Babesia canis isolates of differing vector specificity and pathogenicity to dogs. Parasitology Research 84:544-548.
• [8] Matijatko V., Torti M., Schetters T.P. 2012. Canine babesiosis in Europe: how many diseases? Trends in Parasitology 28:99-105.
• [9] Zygner W., Rapacka G., Gójska-Zygner O., Długosz E., Wedrychowicz H. 2007. Biochemical abnormalities observed in serum of dogs infected with large Babesia in Warsaw (Poland). Polish Journal in Veterinary Sciences 10:245-253.
• [10] Adaszek Ł., Martinez A.C., Winiarczyk S. 2011. The factors affecting the distribution of babesiosis in dogs in Poland. Veterinary Parasitology 181:160-165.
• [11] Cardoso L., Costa A., Tuna J., Vieira L., Eyal O., Yisaschar-Mekuzas Y., Baneth G. 2008. Babesia canis canis and Babesia canis vogeli infections in dogs from northern Portugal. Veterinary Parasitology 156:199-204.
• [12] Solano-Gallego L., Trotta M., Carli E., Carcy B., Caldin M., Furlanello T. 2008. Babesia canis canis and Babesia canis vogeli clinicopathological findings and DNA detection by means of PCR-RFLP in blood from Italian dogs suspected of tick-borne disease. Veterinary Parasitology 157:211-221.
• [13] Kubelová M., Tkadlec E., Bednář M., Roubalová E., Siroký P. 2011. West-to-east differences of Babesia canis canis prevalence in Dermacentor reticulatus ticks in Slovakia. Veterinary Parasitology 180:191-196.
• [14] Milczak A., Riha T., Abramowicz B., Madej E. 2004. Hemostatic disorders during the course of canine babesiosis. Medycyna Weterynaryjna 60:1067-1070.
• [15] Adaszek Ł., Winiarczyk S., Skrzypczak M. 2009. The clinical course of babesiosis in 76 dogs infected with protozoan parasites Babesia canis canis. Polish Journal in Veterinary Sciences 12:81-87.
• [16] Zygner W., Górski P., Wędrychowicz H. 2009. Detection of the DNA of Borrelia afzelii, Anaplasma phagocytophilum and Babesia canis in blood samples from dogs in Warsaw. Veterinary Record 164:465-467.
• [17] Dzięgiel B., Adaszek Ł., Winiarczyk M., García-Bocanegra I., Carbonero A., Dębiak P., Winiarczyk S. 2013. Comparative analysis of 16S RNA nucleotide sequences of Anaplasma phagocytophilum detected in the blood of horses from various parts of Europe. Journal of Medical Microbiology 62:1891-1896.
• [18] Adaszek Ł., Klimiuk P., Skrzypczak M., Górna M., Ziętek J., Winiarczyk S. 2012. The identification of Anaplasma spp. isolated from fallow deer Dama dama on a free-range farm in eastern Poland. Polish Journal in Veterinary Sciences 15:393-394.
• [19] Dzięgiel B., Adaszek Ł., Krzysiak M., Skrzypczak M., Adaszek M., Furmaga B., Winiarczyk S. 2015. The occurrence of Anaplasma phagocytophilum in wild bison from the Bialowieza Primeval Forest in Eastern Poland. Berliner und Münchener Tierärztliche Wochenschrift 128:310-314.
• [20] Dzięgiel B., Kubrak T., Adaszek Ł., Dębiak P., Wyłupek D., Bogucka-Kocka A., Lechowski J., Winiarczyk S. 2014. Prevalence of Babesia canis, Borrelia burgdorferi sensu lato, and Anaplasma phagocytophilum in hard ticks collected from meadows in the Lubelskie Voivodeship eastern Poland. Bulletin of the Veterinary Institute in Puławy 58:29-33.
• [21] Miró G., Montoya A., Roura X., Gálvez R., Sainz A. 2013. Seropositivity rates for agents of canine vectorborne diseases in Spain: a multicentre study. Parasites and Vectors 6:117.
• [22] Müller H., Aysul N., Liu Z., Salih D.A., Karagenc T., Beyer D., Kullmann B., Ahmed J.S., Seitzer U. 2010. Development of a loop-mediated isothermal amplification (LAMP) assay for rapid diagnosis of Babesia canis infections. Transboundary and Emerging Diseases 57:63-65.
• [23] Adaszek Ł., Jankowska M., Kalinowski M., Banach T., Wułupek D., Winiarczyk S. 2013. The loopmediated isothermal amplification assay for rapid diagnosis of Babesia canis canis infections in dogs. Polish Journal in Veterinary Sciences 16:131-133.
• [24] Meiser C., Piechura H., Werner T., Dittmeyer-Schäfer S., Meyer H., Warscheid B., Schaub G., Balczun C. 2010. Kazal-type inhibitors in the stomach of Panstrongylus megistus (Triatominae, Reduviidae). Insect Biochemistry and Molecular Biology 40:345-353.
• [25] Ahmad F., Babalola O., Tak H. 2012. Potential of MALDI-TOF mass spectrometry as a rapid detection technique in plant pathology: identification of plantassociated microorganisms. Analytical and Bioanalytical Chemistry 404:1247-1255.
• [26] Ndao M. 2012. Biomarker discovery in serum/plasma using surface enhanced laser desorption ionization time of flight (SELDI-TOF) mass spectrometry. Methods in Molecular Biology 818:67-79.
• [27] Dzięgiel B., Adaszek Ł., Carbonero A., Łyp P., Winiarczyk M., Dębiak P., Winiarczyk S. 2016. Detection of canine vector-borne diseases in eastern Poland by ELISA and PCR. Parasitology Research 115:1039-1044.
• [28] Adaszek Ł., Winiarczyk S., Puchalski A., Skrzypczak M. 2010. First cases of dog borreliosis in eastern Poland. Veterinarija ir Zootechnika 50:102-104.
• [29] Stokes M.E., Davis C.S., Koch G.G. 2000. Categorical data analysis using the SAS system. 2nd ed., SAS Publishing.
• [30] Yssouf A., Flaudrops C., Drali R., Kernif T., Socolovschi C., Berenger J. M., Raoult D., Parola P. 2013. Matrix-assisted laser desorption ionizationtime of flight mass spectrometry for rapid identification of tick vectors. Journal of Clinical Microbiology 51:522-528.
• [31] Fotso Fotso A., Mediannikov O., Diatta G., Almeras L., Flaudrops C., Parola P., Drancourt M. 2014. MALDI-TOF mass spectrometry detection of pathogens in vectors: the Borrelia crocidurae/Ornithodoros sonrai paradigm. PLoS Neglected Tropical Diseases.doi:10.1371/journal. pntd. 0002984.
• [32] Yssouf A., Almeras L., Terras J., Socolovschi C., Raoult D., Parola P. 2015. Detection of Rickettsia spp in ticks by MALDI-TOF MS. PLoS Neglected Tropical Diseases.doi:10.1371/journal.pntd.0003473.
• [33] Norris S.J. 2006. The dynamic proteome of Lyme disease Borrelia. Genome Biology 7:209.
• [34] Kahlon A., Ojogun N., Ragland S.A., Seidman D., Troese M.J., Ottens A.K., Mastronunzio J.E., Truchan H.K., Walker N.J., Borjesson D.L., Fikrig E., Carlyon J.A. 2013. Anaplasma phagocytophilum Asp14 is an invasin that interacts with mammalian host cells via its C terminus to facilitate infection. Infection and Immunity 81:65-79.
• [35] Kuleš J., Mrljak V., Barić Rafaj R., Selanec J., Burchmore R., Eckersall P.D. 2014. Identification of serum biomarkers in dogs naturally infected with Babesia canis canis using a proteomic approach. BMC Veterinary Research 10:111.
• [36] Adaszek Ł., Puchalski A., Dec M., Winiarczyk S. 2012. Analysis of the culture-derived soluble Babesia canis canis antigens derived from the Polish strains of the parasites. Tierärztliche Praxis. Ausgabe K, Kleintiere/Heimtiere 40:399-403.
• [37] Adaszek Ł., Wernicka-Furmaga R., Winiarczyk S. 2012. Preliminary study on the safety of a new vaccine against canine babesiosis containing soluble parasitic antigen (SPA). Bulletin of the Veterinary Institute in Puławy 56:145-148.
• [38] Adaszek Ł., Banach T., Bartnicki M., Winiarczyk D., Łyp P., Winiarczyk S. 2014. Application the mass spectrometry MALDI-TOF technique for detection of Babesia canis canis infection in dogs. Parasitology Research 13:4293-4295.

Identyfikatory

DOI

10.17420/ap6202.39