Protein differential expression in the latex from Hevea brasiliensis between self-rooting juvenile clones and donor clones

• Autorzy: Li H.L. , Guo D. , Lan F. Y. , Tian W. M. , Peng S. Q.
• Języki publikacji: EN

Abstrakty

EN

To better understand molecular mechanism underlying the difference between self-rooting juvenile clones and donor clones, a proteomic approach was used to profile protein changes in the latex between self-rooting juvenile clones and donor clones. Total soluble proteins were extracted from latex in self-rooting juvenile clones and donor clones. Two-dimensional gel electrophoresis (2-DE) was used to identify proteins that were differentially expressed in self-rooting juvenile clones and donor clones and image analysis was used to determine which proteins were up- or down-regulated. Twenty-four differentially expressed protein spots were identified by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Among the 24 proteins identified, 13 proteins were up-regulated, 11 proteins were decreased in self-rooting juvenile clones. These proteins were classified as carbohydrate and energy metabolism, secondary metabolism, signal translocation, transcriptional regulation-related, protein synthesis and degradation, transport, nucleoside acid process, lipid metabolism. Perhaps, the present study contributes towards an understanding of the molecular mechanism underlying the difference between self-rooting juvenile clones and donor clones.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 5
• Opis fizyczny: p.1853-1859,fig.,ref.

Twórcy

autor

Li H.L.

• Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China

autor

Guo D.

• Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China

autor

Lan F. Y.

• Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China

autor

Tian W. M.

• Key Laboratory of Rubber Biology, Ministry of Agriculture, Institute of Rubber, Chinese Academy of Tropical Agricultural Sciences, Danzhou, 571737, China

autor

Peng S. Q.

• Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China

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