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2013 | 22 | 1 |
Tytuł artykułu

Immune stress up regulates TLR4 and Tollip gene expression in the hypothalamus of ewes

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Bacterial endotoxin, LPS, is recognized by Toll-like receptor-4 (TLR4) and induces a signaling cascade leading to synthesis of proinflammatory cytokines and induction of sickness behavior in animals. Transduction of the TLR4 signal is controlled by a potent negative regulator—Toll-interacting protein. The presented study concerns the effect of intravenously injected LPS on the level of expression of TLR4 and Tollip genes in the hypothalamus of ewes. Endotoxin increased (P < 0.01) cortisol release and expression of TLR4 and Tollip genes in the preoptic area (1.87 ± 0.42 and 1.31 ± 0.15), anterior hypothalamus (1.77 ± 0.22 and 1.27 ± 0.13), medial basal hypothalamus (2.53 0.65 and 1.43 ± 0.15), and median eminence (2.93 ± 0.46 and 1.73 ± 0.10), respectively, in comparison with non-treated animals. Our results show that immune stress increases TLR4 gene expression in the hypothalamus. Increased transcription of Tollip may be an attempt to reduce the effect of TLR4 stimulation.
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  • The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jablonna, Poland
  • The Academy of Cosmetics and Health Care in Warsaw, 00-252 Warsaw, Poland
  • The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jablonna, Poland
  • The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jablonna, Poland
  • Banks W.A., Robinson S.M., 2010. Minimal penetration of lipopolysaccharide across the murine blood-brain barrier. Brain Behav. Immun. 24, 102–109
  • Besedovsky H.O., del Rey A., 1996. Immune-neuro-endocrine interactions: facts and hypotheses. Endocrine Rev. 17, 64–102
  • Bluthé R.M., Castanon N., Pousset F., Bristow A., Ball C., Lestage J., Michaud B., Kelley K.W., Dantzer R., 1999. Central injection of IL-10 antagonizes the behavioural effects of lipopolysaccharide in rats. Psychoneuroendocrinology 24, 301–311
  • Chakravarty S., Herkenham M., 2005. Toll-like receptor 4 on nonhematopoietic cells sustains CNS inflammation during endotoxemia, independent of systemic cytokines. J. Neurosci. 25, 1788–1796
  • Coleman E.S., Elsasser T.H., Kemppainen R.J., Coleman D.A., Sartin J.L., 1993. Effect of endotoxin on pituitary hormone secretion in sheep. Neuroendocrinology 58, 111–122
  • Dadoun F., Guillaume V., Sauze N., Farisse J., Velut J.G., Orsoni J.C., Gaillard R., Oliver C., 1998. Effect of endotoxin on the hypothalamic-pituitary-adrenal axis in sheep. Eur. J. Endocrinology 138, 193–197
  • Dantzer R., 2004. Cytokine-induced sickness behaviour: a neuroimmune response to activation of innate immunity. Eur. J. Pharmacol. 500, 399–411
  • Ebisui O., Fukata J., Tominaga T., Murakami N., Kobayashi H., Segawa H., Muro S., Naito Y., Nakai Y., Masui Y., 1992. Roles of interleukin-1α and -1β in endotoxin-induced suppression of plasma gonadotropin levels in rats. Endocrinology 130, 3307–3313
  • Esposito P., Gheorghe D., Kandere K., Pang X., Connolly R., Jacobson S., Theoharides T.C., 2001. Acute stress increases permeability of the blood–brain-barrier through activation of brain mast cells. Brain Res. 888, 117–127
  • Feng S.Y.S., Yu V.Y.H., Walker A.M., 2007. Endotoxin-induced circulatory changes in the newborn brain: A review. Hong Kong J. Pediat. (New Series) 12, 111–117
  • Kaisho T., Akira S., 2006. Toll-like receptor function and signalling. J. Allerg. Clin. Immunol. 117, 979–987
  • Kokot F., Stupnicki R. (Editors), 1985. Radioimmunological and Radiocompetitive Methods Used in Clinical Studies. (in Polish). 2nd Edition. Warsaw, PZWL
  • Laflamme N., Rivest S., 2001. Toll-like receptor 4: the missing link of the cerebral innate immune response triggered by circulating gram-negative bacterial cell wall components. Feder. Amer. Soc. Exp. Biol. J. 15, 155–163
  • Leon C.G., Tory R., Jia J., Sivak O., Wasan K.M., 2008. Discovery and development of toll-like receptor 4 (TLR4) antagonists: a new paradigm for treating sepsis and other diseases. Pharmaceut. Res. 25,1751–1761
  • Li T., Hu J., Li L., 2004. Characterization of Tollip protein upon Lipopolysaccharide challenge. Mol. Immunol. 41, 85–92
  • Melmed S., 2001. The immuno-neuroendocrine interface. J. Clin. Invest. 108, 1563–1566
  • Muzio M., Bosisio D., Polentarutti N., D’amico G., Stoppacciaro A., Mancinelli R., Van’t Veer C., Penton-Rol G., Ruco L.P., Allavena P., Mantovani A., 2000. Differential expression and regulation of toll-like receptors (TLR) in human leukocytes: selective expression of TLR3 in dendritic cells. J. Immunol. 164, 5998–6004
  • Nguyen M.D., Julien J.P., Rivest S., 2002. Innate immunity: the missing link in neuroprotection and neurodegeneration? Nat. Rev. Neurosci. 3, 216–227
  • Nomura F., Akashi S., Sakao Y., Sato S., Kawai T., Matsumoto M., Nakanishi K., Kimoto M., Miyake K., Takeda K., Akira S., 2000. Cutting edge: Endotoxin tolerance in mouse peritoneal macrophages correlates with down-regulation of surface TollLike receptor 4 expression. J. Immunol. 164, 3476–3479
  • Otte J.M., Cario E., Podolsky D.K., 2004. Mechanisms of cross hyporesponsiveness to toll-like receptor bacterial ligands in intestinal epithelial cells. Gastroenterology 126, 1054–1070
  • Pedchenko T.V., Park G.Y., Joo M., Blackwell T.S., Christman J.W., 2005. Inducible binding of PU.1 and interacting proteins to the Toll-like receptor 4 promoter during endotoxemia. Amer. J. Physiol. – Lung Cell M. Ph. 289, L429–L437
  • Pfaffl M.W., Horgan G.W., Dempfle L., 2002. Relative Expression Software Tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucl. Acids Res. 30, E36
  • Pfaffl M.W., Tichopad A., Prgomet C., Neuvians T.P., 2004. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – excel-based tool using pair-wise correlations. Biotechnol. Lett. 26, 509–515
  • Singh A.K., Jiang Y., 2004. How does peripheral lipopolysaccharide induce gene expression in the brain of rats? Toxicology 201, 197–207
  • Tsatsanis C., Androulidaki A., Alissafi T., Charalampopoulos I., Dermitzaki E., Roger T., Gravanis A., Margioris A.N., 2006. Corticotropin-releasing factor and the urocortins induce the expression of TLR4 in macrophages via activation of the transcription factors PU.1 and AP-1. J. Immunol. 176, 1869–1877
  • Tsuji M., Matsuda H., Miwa H., Miyazaki S., 2003. Antimicrobialinduced release of endotoxin from Pseudomonas aeruginosa: comparison of in vitro and animal models. J. Antimicrob. Chemother. 51, 353–359
  • Turgut M., Erdogan S., Ergin K., Serter M., 2007. Melatonin ameliorates blood-brain barrier permeability, glutathione, and nitric oxide levels in the choroid plexus of the infantile rats with kaolininduced hydrocephalus. Brain Res. 1175, 117–125
  • Wilson A.C., Clemente L., Liu T., Bowen R.L., Meethal S.V., Atwood C.S., 2008. Reproductive hormones regulate the selective permeability of the blood-brain barrier. BBA – Mol. Basis Dis. 1782, 401–407
  • Zhang G., Ghosh S., 2002. Negative regulation of toll-like receptormediated signaling by Tollip. J. Biol. Chem. 277, 7059–7065
  • Zhou Q., Tan X., Wang J., Zhou B., Guo P., 2011. Increased permeability of blood-brain barrier caused by inflammatory mediators is involved in high altitude cerebral edema. Sci. Res. Essays 6, 607–615
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