Flow cytometric assessment of activation of peripheral blood platelets in dogs with normal platelet count and asymptomatic thrombocytopenia

• Autorzy: Zmigrodzka M. , Guzera M. , Winnicka A.
• Języki publikacji: EN

Abstrakty

EN

Platelets play a crucial role in hemostasis. Their activation has not yet been evaluated in healthy dogs with a normal and low platelet count. The aim of this study was to determine the influence of activators on platelet activation in dogs with a normal platelet count and asymptomatic thrombocytopenia. 72 clinically healthy dogs were enrolled. Patients were allocated into three groups. Group 1 consisted of 30 dogs with a normal platelet count, group 2 included 22 dogs with a platelet count between 100 and 200×109/l and group 3 consisted of 20 dogs with a platelet count lower than 100×109/l. Platelet rich-plasma (PRP) was obtained from peripheral blood samples using tripotassium ethylenediaminetetraacetic acid (K3-EDTA) as anticoagulant. Next, platelets were stimulated using phorbol-12-myristate-13-acetate or thrombin, stabilized using procaine or left unstimulated. The expression of CD51 and CD41/CD61 was evaluated. Co-expression of CD41/CD61 and Annexin V served as a marker of platelet activation. The expression of CD41/CD61 and CD51 did not differ between the 3 groups. Thrombin-stimulated platelets had a significantly higher activity in dogs with a normal platelet count than in dogs with asymptomatic thrombocytopenia. Procaine inhibited platelet activity in all groups. In conclusion, activation of platelets of healthy dogs in vitro varied depending on the platelet count and platelet activator.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2016
• Tom: 19
• Numer: 2
• Opis fizyczny: p.407-414,fig.,ref.

Twórcy

autor

Zmigrodzka M.

• Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, ul. Nowoursynowska 159, 02-776 Warsaw, Poland

autor

Guzera M.

• Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, U.K.

autor

Winnicka A.

• Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, ul. Nowoursynowska 159, 02-776 Warsaw, Poland

Bibliografia

• Alberio L, Dale GL (1999) Platelet-collagen interactions: membrane receptors and intracellular signalling pathways. Eur J Clin Invest 29: 1066-1076.
• Beardow AW, Buchanan JW (1993) Chronic mitral valve disease in cavalier King Charles spaniels: 95 cases (1987-1991). J Am Vet Med Assoc 203: 1023-1029.
• Bertazzolo W, Corazzi S, Sesso L, Scarpa P, Ru G, Paltrinieri S (2007) Comparison of methods for determining platelet numbers and volume in Cavalier King Charles spaniels. J Small Anim Pract 48: 556-561.
• Blockmans D, Deckmyn H, Vermylen J (1995) Platelet activation. Blood Rev 9: 143- 156.
• Bode AP, Orton SM, Frye MJ, Udis BJ (1991) Vesiculation of platelets during in vitro aging. Blood 77: 877-895.
• Boudreaux MK, Weiss DJ, Wardrop KJ, Brooks MB, Callan MB, Messick JB, Modiano JF, Moritz A, Raskin RE, Teske E, Wellman ML (2010) Platelets in Schalm’s Veterinary Hematology. Lippincott Williams and Wilkins 2-476.
• Briddon SJ, Melford SK, Turner M, Tubylewicz V, Waston SP (1999) Collagen mediates changes in intracellular calcium in primary mouse megakaryocytes through sykdependent and – independent pathways. Blood 93: 3847-3855.
• Brown SJ, Simpson KW, Baker S, Spagnoletti MA, Elwood CM (1994) Macrothrombocytosis in cavalier King Charles spaniels. Vet Rec 135: 281-283.
• Casonato A, Bertomoro A, Pontara E, Dannhauser D, Lazzaro AR, Girolami A (1994) EDTA dependent pseudothrombocytopenia caused by antibodies against the cytoadhesive receptor of platelet gpIIB-IIIA. J Clin Pathol 47: 625-630.
• Cowan SM, Bartges JW, Gompf RE, Hayes JR, Moyers TD, Snider CC, Gerard DA, Craft RM, Muenchen RA, Carroll RC (2004) Giant platelet disorder in the Cavalier King Charles Spaniel. Exp Hematol 32: 344-350.
• Dachary-Prigent J, Freyssinet JM, Pasquet JM, Carron JC, Nurden AT (1993) Annexin V as a probe of aminophospholipid exposure and platelet membrane vesiculation: a flow cytometry study showing a role for free sulfhydryl groups. Blood 10: 2554-2565.
• Davis B, Toivio-Kinnucan M, Schuller S, Boudreaux MK (2008) Mutation in beta1-tubulin correlates with macrothrombocytopenia in Cavalier King Charles Spaniels. J Vet Intern Med 22: 540-545.
• Idriss NK, Blann AD, Sayed DM, Gaber MA, Hassen HA, Kish YT (2014) Circulating Endothelial Cells and Platelet Microparticles in Mitral Valve Disease With and Without Atrial Fibrillation. Angiology 66: 631-637.
• Lara-Garcia A, Couto CG, Iazbik MC, Brooks MB (2008) Postoperative Bleeding in Retired Racing Greyhounds. J Vet Intern Med 22: 525-533.
• Lebrec H, O’Lone R, Freebern W, Komocsar W, Moore P (2012) Survey: immune function and immunotoxicity assessment in dogs. J Immunotoxicol 9: 1-14.
• Moritz A, Walcheck BK, Weiss DJ (2003) Flow cytometric detection of activated platelets in the dog. Vet Clin Pathol 32: 6-12.
• Olsen LH, Kristensen AT, Haggstrom J, Jensen AL, Klitgaard B, Hansson H, Pedrsen HD (2001) Increased platelet aggregation response in Cavalier King Charles Spaniels with mitral valve prolapse. J Vet Intern Med 15: 209-216.
• Philips DR, Charo IF, Parise LV, Fitzgerald LA (1988) The platelet membrane glycoprotein IIb-IIIa complex. Blood 71: 831-843.
• Poncz M, Eisman R, Heidenreich R, Silver SM, Vilaire G, Surrey S, Schwartz E, Bennett JS (1987) Structure of the platelet membrane glycoprotein IIb. Homology to the alpha subunits of the vitronectin and fibronectin membrane receptors. J Biol Chem 262: 8476-8482.
• Preissner KT, Seiffert D (1998) Role of vitronectin and its receptors in haemostasis and vascular remodeling. Thromb Res 89: 1-21.
• Schlossman SF, Boumsell L, Gilks W, Harlan JM, Kishimoto T, Morimoto C, Ritz J, Shaw S, Silverstein R, Springer T (1995) Leukocyte Typing V. New York: Oxford University Press, pp 283-290.
• Singh MK, Lamb WA (2005) Idiopathic thrombocytopenia in Cavalier King Charles Spaniels. Aust Vet J 83: 700-703.
• Steiss J, Brewer WG, Welles E, Wright J (2000) Hematologic and serum biochemical reference values in retired Greyhounds. Compend Cont Educ Pract Vet 22: 243-248.
• Watała C, Boncler M, Golanski J, Koziołkiewicz W, Walkowiak B, Cierniewski CS (1999) Release of Calcium and P-Selectin from Intraplatelet Granules Is Hampered by Procaine. Thromb Res 94: 1-11.
• Whiss PA, Andersson RG, Srinivas U (1998) Kinetics of platelet P-selectin mobilization: concurrent surface expression and release induced by thrombin or PMA, and inhibition by the NO donor SNAP. Cell Adhes Commun 6: 289-300.
• Wilkerson MJ, Shuman W (2001) Alterations in normal canine platelets during storage in EDTA anticoagulated Blood. Vet Clin Pathol 30: 107-113.
• Zucker MB, Nachmias VT (1985) Platelet activation. Arteriosclerosis 5: 2-18.

Identyfikatory

DOI

10.1515/pjvs-2016-0051