Influence of endogenous and exogenous RNases on the variation of pollen cytosolic-free Ca2+ in Pyrus serotina Rehd

• Autorzy: Xu G. , Zhang S. , Yang Y. , Zhao C. , Wolukau J. N.
• Języki publikacji: EN

Abstrakty

EN

The objective of this study was to examine whether S-RNase plays a specific role in the pre-germinated Pyrus pollen. Effects of exogenous RNase and endogenous S-RNase on concentration of cytosolic-free calcium ([Ca⁺²]i) variation of pre-germinated Pyrus pollen were studied. [Ca⁺²]i variation caused by different Rnases were complex. In 1 h after being cultured, exogenous RNase, RNase T1 and RNase A, and endogenous incompatible ‘Hohsui’ RNase promoted the [Ca⁺²]i of ‘Hohsui’ pollen. Acid proteins of ‘Hohsui’ had no remarkable influence on the [Ca⁺²]i of self-pollen. Endogenous compatible ‘Kohsui’ RNase reduced the [Ca⁺²]i of ‘Hohsui’ pollen, but compatible ‘Hohsui’ RNase can stimulate the [Ca⁺²]i of ‘Kohsui’ pollen. RNase T1, RNase A and incompatible ‘Kohsui’ S-RNase can also make ‘Kohsui’ pollen [Ca⁺²]i increase. Different from ‘Hohsui’ pollen, acid proteins of ‘Hohsui’ pull down the ‘Kohsui’ pollen [Ca⁺²]i remarkably. Conclusion can be made that during the prophase of pollen germination, endogenous S-RNase has no specific effect on pollen [Ca⁺²]i changes.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2008
• Tom: 30
• Numer: 2
• Opis fizyczny: p.233-241,fig.,ref.

Twórcy

autor

Xu G.

• College of Horticulture, Nanjing Agricultural University, 210095 Nanjing, Jiangsu, People's Republic of China
• College of Life Science, Nanjing University, Nanjing, 210093 Nanjing, Jiangsu, People's Republic of China

autor

Zhang S.

• College of Horticulture, Nanjing Agricultural University, 210095 Nanjing, Jiangsu, People's Republic of China

autor

Yang Y.

• College of Life Science, Nanjing University, Nanjing, 210093 Nanjing, Jiangsu, People's Republic of China

autor

Zhao C.

• College of Horticulture, Nanjing Agricultural University, 210095 Nanjing, Jiangsu, People's Republic of China

autor

Wolukau J. N.

• Department of Horticulture, Egerton University, 536 Njoro, Kenya

Bibliografia

• Ai Y, Kron E, Kao T-H (1991) S-alleles are retained and expressed in a self-compatible cultivar of Petunia hybrida. Mol Gen Genet 230:353–358
• Anderson M, McFadden G, Bernatzky R et al (1989) Sequence variability of three alleles of the self-incompatibility gene of Nicotiana alata. Plant Cell 1:483–491
• Bernatzky R, Glaven RH, Rivers BA (1995) S-related protein can be recombined with self-compatibility in interspecific derivatives of Lycopersicon. Biochem Genet 33:215–225
• Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantitation of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–245
• Franklin-Tong VE, Ride JP, Read ND et al (1993) The selfincompatibility response in Papaver rhoeasis mediated by cytosolic free calcium. Plant J 4:163–177
• Franklin-Tong VE, Holdaway-Clarke TL, Straatman KR et al (2002) Involvement of extracellular calcium influx in the self-incompatibility response of Papaver rhoeas. Plant J 29(3):333–345
• de Graaf BHJ, Lee C, McClure BA et al (2006) Cellular Mechanisms for Pollen Tube Growth Inhibition in Gametophytic Selfincompatibility. Plant Cell Monographs 3:1861–1370
• Grosberg R (1988) The evolution of allorecognition specificity in clonal invertebrates. Q Rev Biol 63:377–412
• Hancock CN, Kent L, McClure BA (2005) The stylar 120 kDa glycoprotein is required for S-specific pollen rejection in Nicotiana. Plant J 43(5):716–723
• Hiratsuka S, Okada Y, Kawai Y et al (1995) Stylar basic proteins corresponding to 5 self-incompatibility alleles of Japanese pears. J Jpn Soc Hortic Sci 64:471–478
• Hiratsuka S, Zhang SL, Nakagawa E et al (2001) Selective inhibition of the growth of incompatible pollen tubes by S-protein in the Japanese pear. Sex Plant Reprod 13:209–215
• Jahnen W, Batterham MP, Clarke AE et al (1989) Identification, isolation, and N-terminal sequencing of style glycoproteins associated with self-incompatibility in Nicotiana alata. Plant Cell 1:493–499
• Kim HT, Hirata Y, Nou IS (2002) Determination of S-genotypes of pear (Pyrus pyrifolia) cultivars by S-RNase sequencing and PCR-RFLP analyses. Mol Cells 13(3):444–451
• Kim MH, Shin DI, Park HS et al (2001) In vitro function of S Rnases in Lycopersicon peruvianum. Mol Cells 12(3):329–335
• Kronstad J, Leong S (1990) The b mating-type locus of Ustilago maydis contains variable and constant regions. Genes Dev 4:1384–1395
• Matton DP, Luu DT, Xike Q et al (1999) Production of an S-RNase with dual specificity suggests a novel hypothesis for the generation of new S alleles. Plant Cell 11:2087–2098
• McClure BA, Mou B, Canevascini S et al (1999) A small asparaginerich protein required for S-allele-specific pollen rejection in Nicotiana. Proc Natl Acad Sci USA 96:13548–13553
• McClure BA, Franklin-Tong V (2006) Gametophytic self-incompatibility: understanding the cellular mechanisms involved in ‘‘self’’ pollen tube inhibition. Planta 224(2):233–245
• McClure BA, Gray JE, Anderson MA et al (1990) Self-incompatibility in Nicotiana alata involves degradation of pollen rRNA. Nature 347:757–760
• de Nettancourt D (1977) Incompatibility in angiosperms. Springer, New York
• O’Brien M, Kapfer C, Major G et al (2002) Molecular analysis of the stylar-expressed Solanum chacoense asparagine-rich protein family related to the HT modifier of gametophytic self-incompatibility in Nicotiana. Plant J 32:1–12
• Roiz L, Ozeri U, Goren R et al (2000) Characterization of Aspergillus nige B-1 RNase and its inhibitory effect on pollen germination and pollen tube growth in selected tree fruit. J Am Soc Hortic Sci 125:9–14
• Takasaki T, Okada K, Castillo C et al (2004) Sequence of the S9-RNase cDNA and PCR-RFLP system for discriminating S1- to S9-allele in Japanese pear. Euphytica 135(2):157–167
• Thomas SG, Franklin-Tong VE (2004) Self-incompatibility triggers programmed cell death in Papaver pollen. Nature 429:249–250
• Tsukamoto T, Ando T, Kokubun H et al (2003) Breakdown of selfincompatibility in a natural population of Petunia axillaris caused by a modifier locus that suppresses the expression of an S-RNase gene. Sex Plant Reprod 15:255–263
• Wilson CM (1971) Plant nuclease III. Polyacrylamide gel electrophoresis of corn ribonucleases isoenzymes. Plant Physiol 48:64–68
• Xu GH, Zhang SL, Zhang CY (2003) Changes in free Ca⁺² distribution in pollen cell after self- and cross-pollination in Pyrus serotina Rehd. J Plant Physiol Mol Biol 29(2):97–103 (in Chinese with English abstract)
• Zhang SL, Hiratsuka S, Xu GH et al (2001) Expression and possible role of S4 (S4 SM) genes in the styles of self-incompatible pear cultivar and its self-compatible mutant. Acta Botanica Sinica 43(11):1172–1178 (in Chinese with English abstract)
• Zhang SL, Hiratsuka S (2000a) Effects of the stylar S-glycoproteins on the pollen germination and the tube growth in pears (Pyrus serotina Rhed.) in vitro. Acta Horticulturae Sinica 27(4):251–256 (in Chinese with English abstract)
• Zhang SL, Hiratsuka S (2000b) Varietal differences in S-protein concentration and pollen tube growth in developing style of Japanese pears. HortScience 35(5):917–920
• Zhang WH, Rengel Z (1998) Determination of intracellular Ca⁺² in cells of intact wheat root: loading of acetoxymethyl ester of fluo-3 under low temperature. Plant J 15(1):147–151

Uwagi

Rekord w opracowaniu