Plant endophytic fungi as a source of paclitaxel

• Autorzy: Michalczyk A. , Cieniecka-Roslonkiewicz A. , Cholewinska M.

Warianty tytułu

PL

Roślinne grzyby endofityczne jako źródło paklitakselu

• Języki publikacji: EN

Abstrakty

EN

Endophytic fungi were isolated from different parts of the plants Corylus avellana (Corylaceae) and Ocimum basilicum (Lamiaceae) and then identified to the genus level based on the morphology of the fungal culture and the characteristics of the spores. The fungicidal and antitumor activity of isolates and extracts obtained from them was determined. We found that a few isolates from Corylus avellana and Ocimum basilicum produced metabolites that inhibited the growth of Oomycetes fungi to a highly significant extent. In the potato disc bioassay only an extract from the isolate C-9 showed an inhibitory action in tumor development. Paclitaxel presence in extract from the isolate C-9 was confirmed using the thin layer chromatography method (TLC) and UV-VIS spectroscopic analysis. The above results indicated that the tested samples revealed an antitumor activity. The detection of paclitaxel in the isolate C-9 suggests that the antitumor activity resulted probably from the presence of this taxane in the tested material.

PL

Endofity grzybowe izolowano z różnych części tkanek Corylus avellana (Corylaceae) i Ocimum basilicum (Lamiaceae). Następnie przypisywano do rodzaju w oparciu o cechy morfologiczne i budowę struktur zarodnikujących. Sprawdzano aktywność grzybobójczą oraz przeciwnowotworową izolatów oraz uzyskanych z nich ekstraktów. Stwierdzono, że niektóre izolaty z Corylus avellana i Ocimum basilicum wytwarzają metabolity hamujące w znacznym stopniu wzrost grzybów z klasy Oomycetes. W badaniu na krążkach ziemniaków, tylko ekstrakt z izolatu C-9 wykazywał działanie hamujące rozwój tumorów. Obecność paklitakselu w ekstrakcie z izolatu C-9 potwierdzono, stosując metodę chromatografii TLC oraz analizy spektroskopowej UV-VIS. Powyższe wyniki sugerują, że grzyby endofityczne mogą wytwarzać paklitaksel, który jest odpowiedzialny za aktywność przeciwnowotworową badanych próbek i że grzyby endofityczne mogą znaleźć zastosowanie do produkcji paklitakselu w procesie fermentacji.

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2014
• Tom: 60
• Numer: 4
• Opis fizyczny: p.22-33,fig.,ref.

Twórcy

autor

Michalczyk A.

• Institute of Industrial Organic Chemistry, Annopol 6, 03-236 Warsaw, Poland

autor

Cieniecka-Roslonkiewicz A.

• Institute of Industrial Organic Chemistry, Annopol 6, 03-236 Warsaw, Poland

autor

Cholewinska M.

• Institute of Industrial Organic Chemistry, Annopol 6, 03-236 Warsaw, Poland

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Identyfikatory

DOI

10.1515/hepo-2015-0002