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2012 | 34 | 5 |
Tytuł artykułu

Molecular characteristics and expression of cytoplasmic genes in cytoplasmic male sterility of pepper (Capsicum annuum L.)

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
This study compared the sequence variations and expressions of 12 chloroplastic and 8 mitochondrial genes in three pepper (Capsicum annuum L.) cytoplasmic male sterile (CMS) lines, their maintainers and two control cultivars. The results showed that the three CMS lines were highly similar in chloroplastic and mitochondrial fragment sequences, with average similarities of 96.81 and 98.73 %, respectively, and their chloroplastic trnH–psbA intergenic spacer, photosystem II 47 kDa protein (psbB) genes, mitochondrial apocytochrome b (cob) and RNAD fragments have 1, 9, 8 and 7 distinct sites from the maintainer lines, respectively, and could be used as informative sites to distinguish CMS lines from the maintainer lines. Meanwhile, the expressions of mitochondrial cob, RNAD and pvs in the reproductive organs (flowers) of CMS lines are different from those of the maintainer lines, but their expressions in the vegetative organs (roots and leaves) are similar. The results indicate that cytoplasmic DNA polymorphisms are rare in CMS lines, and mitochondrial cob, RNAD and pvs genes are closely related to pollen abortion.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
34
Numer
5
Opis fizyczny
p.2033-2037,fig.,ref.
Twórcy
autor
  • Vegetable Institution of Hunan Academy of Agricultural Science, 410125 Changsha, Hunan, China
  • Key Laboratory of Human Province for Study and Utilization of Ethnic Medicinal Plants Resources, Huaihua University, 418008 Huaihua, Hunan, China
  • Department of Life Sciences, Huaihua University, 418008 Huaihua, Hunan, China
autor
  • Vegetable Institution of Hunan Academy of Agricultural Science, 410125 Changsha, Hunan, China
autor
  • Vegetable Institution of Hunan Academy of Agricultural Science, 410125 Changsha, Hunan, China
autor
  • Vegetable Institution of Hunan Academy of Agricultural Science, 410125 Changsha, Hunan, China
Bibliografia
  • Ashutosh Kumar P, Kumar VD, Sharma PC, Prakash S, Bhat SR (2008) A novel orf108 co-transcribed with the atpA gene is associated with cytoplasmic male sterility in Brassica juncea carrying moricandia arvensis cytoplasm. Plant Cell Physiol 49:284–289
  • Fujii S, Toriyama K (2008) DCW11, down-regulated gene 11 in cw-type cytoplasmic male sterile rice, encoding mitochondrial protein phosphatase 2C is related to cytoplasmic male sterility. Plant Cell Physiol 49:633–640
  • Gehlhar SB, Simons KJ, Maan SS, Klanian SF (2005) Genetic analysis of the species cytoplasm specific gene (scsd) derived from durum wheat. J Hered 96:404–409
  • Hanson MR (1991) Plant mitochondrial mutations and male sterility. Annu Rev Genet 25:461–486
  • Hanson MR, Bentolila S (2004) Interactions of mitochondrial and nuclear genes that affect male gametophyte development. Plant Cell 16:154–169
  • Johns C, Lu MQ, Lyznik A, Mackenzie SA (1992) Mitochondrial DNA sequence is associated with abnormal pollen development in cytoplasmic male sterile bean plants. Plant Cell 4:435–449
  • Kim DS, Kim DH, Yoo JH, Kim BD (2006) Cleaved amplified polymorphic sequence and amplified fragment length polymorphism markers linked to the fertility restorer gene in chili pepper (Capsicum annuum L.). Mol Cells 21:135–140
  • Kress WJ, Wurdack KJ, Zimmer EA, Weigt LA, Janzen DH (2005) Use of DNA barcodes to identify flowering plants. Proc Natl Acad Sci USA 102:8369–8374
  • Lin XY, Zhou PJ, Hang QY, Guan HX, Zhu YG (2000) Isolation and sequence analysis of a mitochondrial DNA fragment associated with CMS in Hong Lian type rice. Acta Biol Exp Sin 33:151–155
  • Liu F, Cui XQ, Horner HT, Weiner H, Schnable PS (2001) Mitochondrial aldehyde dehydrogenase activity is required for male fertility in maize. Plant Cell 13:1063–1078
  • Liu ZY, Peter SO, Long MH, Weingartner U, Stamp P, Kaeser O (2002) A PCR assay for rapid discrimination of sterile cytoplasm types in maize. Crop Sci 42:566–569
  • Ou LJ, Huang GW, Li WJ, Kang GP, Chen JL, Luan S, Chen LB (2009) Chloroplast DNA polymorphism in different types of cytoplasmic male sterile rice. Biol Plantarum 53:593–596
  • Satoh Y, Ito K, Mikami T (1989) PCR amplification of the mitochondrial DNA sequence specific to the male-sterile and normal cytoplasm in onion. J Hered 80:143–145
  • Saxena KB, Ravikoti VK, Dalvi VA, Pandey LB, Gaddikeri G (2010) Development of cytoplasmic–nuclear male sterility, its inheritance, and potential use in hybrid pigeonpea breeding. J Hered 101:497–503
  • Shaw J, Lickey EB, Beck JT, Farmer SB, Liu WS, Miller J, Siripun KC, Winder CT, Schilling E, Randall L (2005) The tortoise and the hare II: relative utility of 21 non-coding chloroplast DNA sequences for phylogenetic analysis. Amer J Bot 92:142–166
  • Wang LH, Zhang BX, Lefebvre V, Huang SW, Daubèze AM (2004) QTL analysis of fertility restoration in cytoplasmic male sterile pepper. Theor Appl Genet 109:1058–1063
  • Wu ZM, Hu KL, Qiao AM (2009) Differential expression analysis in flower buds of CMS and its maintainer line of Capsicum annuum L. Acta Hort Sin 36:1311–1316
  • Yao FY, Li GX, Zhu CX, Wen FJ (2001) RAPD analysis for mitochondrial DNAs of the BT cytoplasmic male sterile (CMS) line and its associated lines in rice (Oryza sativa). Acta Bot Boreal Occident Sin 21:839–843
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-301aee75-149b-4470-bdcb-db9a60e59972
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