Pre- and postnatal differential gene expression with relevance for meat and carcass traits in pigs - a review

• Autorzy: Wimmers K. , Murani E. , Ponsuksili S.

Warianty tytułu

PL

Pre- i postnatalne różnice w ekspresji genów w odniesieniu do cech mięsa i tuszy u trzody chlewnej – praca przeglądowa

• Języki publikacji: EN

Abstrakty

EN

Pre- and postnatal processes determine the final outcome of breeding of pigs in terms of traits related to carcass and meat quality at slaughter. In particular, the number of myofibers and to a large extent their metabolic and contractile properties, which also influence their size, are determined prenatally during the process of myogenesis. By this, postnatal muscle growth and parameters of meat quality are modulated. The metabolic balance, biochemical and biophysical preslaughter properties of muscle prior to slaughter determine the process of maturation of muscle to meat.Thus, differential regulation of the abundance of transcripts of biological networks in prenatal and postnatal muscle affect biochemical processes of meat maturation. In general, because the traits of interest are typically not expressed at prenatal stages, no direct relationship between phenotype and gene expression pattern can be established. However, trait-related differential expression within any prenatal developmental stage can be assessed based on known estimated breeding values, known QTL-genotypes and/or based on breed differences. Expression profiles of muscle at slaughter can directly be linked to meat quality. A suitable experimental design of “matched samples” is the discordant sib pair design. Here it is exemplarily discussed that differentially expressed transcript profiles of M. longissimus dorsi at prenatal and postnatal stages offer an insight into the biological processes in the live muscle that affect the process of meat maturation and finally meat quality.

PL

Procesy pre- i postnatalne determinują ostateczny wynik w produkcji mięsa wieprzowego, tak w odniesieniu do jakości tuszy, jak i samego mięsa. Szczególnie, liczba miofibryli, ale także w znacznym stopniu ich właściwości metaboliczne i zdolności skurczowe, determinowane są prenatalnie w czasie miogenezy. procesy prenatalne oddziałują także na postnatalny przyrost mięśni i jakość mięsa. Równowaga metaboliczna, biochemiczne i biofizyczne właściwości mięsa przed ubojem warunkują dalszy proces dojrzewania mięsa. Stąd, zróżnicowana regulacja mnogości transkryptów w szlakach biologicznych w pre- i postnatalnej mięśniówce wpływa na biochemiczne procesy dojrzewania mięsa. Ogólnie, ponieważ cechy mięsa, którymi jesteśmy zainteresowani, nie są ujawniane w stadium prenatalnym, nie można wyznaczyć bezpośrednich zależności między fenotypem a modelem ekspresji genów. Jednakże prenatalne zróżnicowanie ekspresji genów związane z poszczególnymi cechami może być opisane w oparciu o znane wartości hodowlane, genotypy QTL i różnice rasowe. Profile ekspresji w mięśniach mogą być bezpośrednio związane z jakością mięsa. Odpowiednim układem doświadczalnym w tym przypadku jest analiza różnic między rodzeństwem (discordant sib pair design). W niniejszym opracowaniu dyskutowane są przykładowo zróżnicowane profile transkrypcyjne w m. longissimus dorsi w stadium prei postnatalnym, jako umożliwiające wgląd w procesy biologiczne w funkcjonujących mięśniach, które następnie wpływają na proces dojrzewania mięsa, a tym samym na jego jakość.

Słowa kluczowe

EN

prenatal process; postnatal process; gene expression; meat; carcass; pig; microarray; transcriptome; meat quality; muscle; myogenesis

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2010
• Tom: 28
• Numer: 2
• Opis fizyczny: p.115-122,ref.

Twórcy

autor

Wimmers K.

• Research Institute for the Biology of Farm Animals, FBN Dummerstorf, Wilhelm-Stahl-Allee 2, 18916 Dummerstorf, Germany

autor

Murani E.

autor

Ponsuksili S.

Bibliografia

• BAIBAI Q., mcGILLIVRAY ., Da COSTA N., DORNAN S., EVANS G., STEAR M.J., CHANG K.C. 2003 – Development of a porcine skeletal muscle cDNA microarray: analysis of differentia transcript epresssion in phenotypically distinct muscles. BMC Genomics 4, 8.
• BERNARD C., CASSAR-MALEK I., Le CUNFF M., DUBROEUCQ H., RENAND G.,HOCQUETTE J.F., 2007 – New indicators of beef sensory quality revealed by expression of specific genes. Journal of Agricultural and Food Chemistry 55, 5229-5237.
• CAGNAZZO M., Te PAS M.F., PRIEM J., de WIT A.A., POOL M.H., DAVOLI R., RUSSO V. 2006 – Comparison of prenatal muscle tissue expression profiles of two pig breeds differing in muszle characteristics. Journal of Animal Science 84, 1-10.
• DAVOLI R., COLOMBO M., SCHIAVINA S., FONTANESI L., BUTTAZONI L., RUSSO V.,2007 – Transcriptome analysis of porcine skeletal muscle: differentially expressed genes in Italia Large White pigs with divergent values for glycolytic potential. Italian Journal of Animal Science 6 (Suppl. 1), 113-115.
• ERNST C.W., RILINGTON V.D., RANEY N.E., YAO J., SIPKOWSKY S.S., SAAMA P.M., TEMPELMAN R.J., COUSSENS P.M., 2002 – Use of cdna microarrays to detect differentially expressed genes in developing pig skeletal muscle. Abstracts for the Plant and Animal Genomes X Conference, San Diego, USA, 702.
• LIU G., JENNEN D.G., THOLEN E., JUENGST H., KLEINWACHTER T., HOLKER M.,TESFAYE D., UN G., SCHREINEMACHERS H.J., MURANI E., PONSUKSILI S., KIM J.J.,SCHELLANDER K., WIMMERS K., 2007 – A genome scan reveals QTL for growth, fatness, leanness and meat quality in a Duroc-Pietrain resource population. Animal Genetics 38, 241-252.
• LIU G., KIM J.J., JONAS E., WIMMERS K., PONSUKSILI S., MURANI E., PHATSARA C.,THOLEN E., JUENGST H., TESFAYE D., CHEN J.L., SCHELLANDER K., 2008 – Combined line-cross and half-sib QTL analysis in Duroc-Pietrain population. Mammalian Genome 19, 429-438.
• MAAK S., JAESERT S., NEUMANN K., YERLE M., LENGERKEN G.V., 2001 – Isolation of expressed sequence tags of skeletal muscle of neonatal healthy and splay leg piglets and mapping by somatic cell hybrid analysis. Animal Genetics 32, 303-307.
• MURANI E., MURANIOVA M., PONSUKSILI S., SCHELLANDER K., WIMMERS K., 2007 – Identification of genes differentially expressed during prenatal development of skeletal muscle In two pig breeds differing in muscularity. BMC Developmental Biology 7, 109.
• PONSUKSILI S., JONAS E., MURANI E., PHATSARA C., SRIKANCHAI T., WALZ C.,SCHWERIN M., SCHELLANDER K., WIMMERS K., 2008b – Trait correlated expression combined with expression QTL analysis reveals biological pathways and candidate genes affecting water holding capacity of muscle. BMC Genomics 9, 367.
• PONSUKSILI S., MURANI E., PHARSARA C., JONAS E., WALZ C., SCHWERIN M., SCELLANDER K., WIMMERS K., 2008a – Expression profiling of muscle reveals transcripts differentially expressed in muscle that affect water-holding capacity of pork. Journal of Agricultural and Food Chemistry 56, 10311-10317.
• PONSUKSILI S., MURANI E., SCHELLANDER K., SCHWERIN M., WIMMERS K., 2005 – Identification of functional candidate genes for body composition by expression analyses and evidencing impact by association analysis and mapping. Biochemica and Biophysica Acta 1730,31-40.
• Ponsuksili S., Murani E., Walz C., Schwerin M., Wimmers K., 2007 - Pre- and postnatal hepatic gene expression profiles of two pig breeds differing in body composition: insight into pathways of metabolic regulation. Physiological Genomics 29, 267-279.
• PONSUKSILI S., WIMMERS K., SCHMOLL F., ROBIC A., SCHELLANDER K., 2000 – Porcie ESTs detected by differential display representing possible candidates for carcass traits, Journal of Animal Breeding and Genetics 117, 25-35.
• SEO K., BEEVER J.E., 2001 – Monitoring gene expression in swine skeletal muscle. Abstracts for the Plant and Animal Genomes IX Conference, San Diego, USA, 207.
• Te PAS M., WIT A.A.C., de PRIEM J., CAGNAZZO M., DAVOLI R., RUSSO V., POOL M.H.,2005 – Transcriptome expression profiles in prenatal pigs in relation to myogenesis. Journal of Muscle Research and Cell Motility 26, 157-165.
• WIMMERS K., FIEDLER I., HARDGE T., MURANI E., SCHELLANDER K., PONSUKSILI S.,2006 – QTL for microstructural and biophysical muscle properties and body composition in pigs.BMC Genetics 7, 15.
• WIMMERS K., MURANI E., PONSUKSILI S., YERLE M., SCHELLANDER K., 2002b – Detection of quantitative trait loci for carcass traits in the pig by using AFLP. Mammalian Genome 13, 206-210.
• WIMMERS K., MURANI E., Te PAS M.F., CHANG K.C., DAVOLI R., MERKS J.W., HENNE H.,MURANIOVA M., da COSTA N., HARLIZIUS B., SCHELLANDER K., BOLL I., BRAGLIA S.,de WIT A.A., CAGNAZZO M., FONTANESI L., PRINS D., PONSUKSILI S., 2007 – Associations of functional candidate genes derived from gene-expression profiles of prenatal porcine muscle tissue with meat quality and muscle deposition. Animal Genetics 38, 474-484.
• WIMMERS K., Te PAS M.F.W., CHANG K.C., DAVOLI R., MERKS J.W.M., WORNER R.,EPING H., MURANI E., PONSUKSILI S., SCHELLANDER K., PRIEM J.M., CAGNAZZO M.,FONTANESI L., LAMA B., HARLIZIUS B., HENNE H., 2002a – An European initiative towards identification of genes controlling pork quality. Proceedings of the 7th WCGALP, Montpellier,France, 11-13.
• WU G, BAZER F.W., Datta S., Gao H., Johnson G.A., Lassala A., Li P., Satterfield M.C., Spencer T.E., 2008 - Intrauterine growth retardation in livestock: Implications, mechanisms and solutions. Archiv fuer Tierzucht 51 (Special Issue), 1-7.
• ZHAO S., NETTLETON D., LIU W., FITZIMMONS C., ERNST C.W., RANEY N.E., TUGGLE C.K., 2003 – cDNA macroarray analyses of differential gene expression in porcine fetal and postnatal muscle. Abstracts for the Plant and Animal Genomes XI Conference, San Diego, USA, 623.
• ZHAO S., PAN P., YANG S., LIU B., Li K., 2001 – Identification of differentially expressed genes in longissimus muscle between Duroc and Erhualian pig by mRNA differential display. Abstracts for the Plant and Animal Genomes IX Conference, San Diego, USA, 602.