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2014 | 36 | 10 |
Tytuł artykułu

The response of rbcL , rbcS and rca genes in cucumber (Cucumis sativus L.) to growth and induction light intensity

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Low light is one of the most important factors affecting cucumber growth in winter solar greenhouses in northern China. Whether induction light intensity has an effect on expressions of rbcL, rbcS and rca genes in cucumber leaves grown under low light for long periods of time remains unclear. Here, two genotypes of cucumber were used to determine the effects of growth and induction light levels on photosynthetic induction. It was found that regardless of growth and induction light intensity, the transcript levels of rbcL, rbcS and rca increased quickly once the plant was exposed to light. The variations of the photosynthesis (PN), stomatal conductance (gₛ), actual photosystem II efficiency (ΦPS II) and Rubisco activity were similar to transcript levels. Plants grown at a photon flux density (PFD) of 100 µmol m⁻² s⁻¹ had lower PN, and initial and total Rubisco activity than those grown at 600 µmol m⁻² s⁻¹ , regardless of induction PFD; for plants grown at the same PFD, PN, gₛ and Rubisco activation rate induced under PFD of 100 µmol m⁻² s⁻¹ were lower than those under 600 µmol m⁻² s⁻¹ . Rubisco, especially RCA, played a great role on photosynthesis during photosynthetic induction. Compared with Jinyan No. 2, Deltastar may possess potential low-light tolerance with a lower decrease of PN, and initial and total Rubisco activities when grown and induced at 100 µmol m⁻² s⁻¹ than 600 µmol m⁻² s⁻¹ . Moreover, the mechanism of Rubisco activase in regulating Rubisco during photosynthetic induction is also discussed.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
36
Numer
10
Opis fizyczny
p.2779-2791,fig.,ref.
Twórcy
autor
  • Beijing Key Laboratory of Growth and Development Regulation for Protected Vegetable Crops, College of Agriculture and Biotechnology, China Agricultural University, Beijing, 100193, People’s Republic of China
  • Shandong Key Laboratory of Greenhouse Vegetable Biology, Shandong Branch of National Vegetable Improvement Center, Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Jinan, 250100, People’s Republic of China
autor
  • Beijing Key Laboratory of Growth and Development Regulation for Protected Vegetable Crops, College of Agriculture and Biotechnology, China Agricultural University, Beijing, 100193, People’s Republic of China
autor
  • Plant Science and Technology Department, Beijing University of Agriculture, Beijing, 102206, People’s Republic of China
autor
  • Beijing Key Laboratory of Growth and Development Regulation for Protected Vegetable Crops, College of Agriculture and Biotechnology, China Agricultural University, Beijing, 100193, People’s Republic of China
autor
  • Beijing Key Laboratory of Growth and Development Regulation for Protected Vegetable Crops, College of Agriculture and Biotechnology, China Agricultural University, Beijing, 100193, People’s Republic of China
autor
  • Beijing Key Laboratory of Growth and Development Regulation for Protected Vegetable Crops, College of Agriculture and Biotechnology, China Agricultural University, Beijing, 100193, People’s Republic of China
autor
  • Beijing Key Laboratory of Growth and Development Regulation for Protected Vegetable Crops, College of Agriculture and Biotechnology, China Agricultural University, Beijing, 100193, People’s Republic of China
Bibliografia
  • Bilger W, Bjorkman O (1990) Role of the xanthophyll cycle in photoprotection elucidated by measurements of light-induced absorbency changes, fluorescence and photosynthesis in leaves of Hedera-canariensis. Photosynth Res 25:173–185
  • Bradford MM (1976) A rapid and sensitive for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 72:248–254
  • Butz ND, Sharkey TD (1989) Activity ratios of ribulose-1,5-bisphosphate carboxylase accurately reflect carbamylation ratios. Plant Physiol 89:735–739
  • Carmo-Silva AE, Salvucci ME (2013) The regulatory properties of Rubisco activase differ among species and affect photosynthetic induction during light transitions. Plant Physiol 161:1645–1655
  • Chen JW, Zhang QA, Li XS, Cao KF (2011) Steady and dynamic photosynthetic responses of seedlings from contrasting successional groups under low-light growth conditions. Physiol Plant 141:84–95
  • Cheng LL, Fuchigami LH (2000) Rubisco activation state decreases with increasing nitrogen content in apple leaves. J Exp Bot 51:1687–1694
  • Ernstsen J, Woodrow IE (1997) Responses of Rubisco activation and deactivation rates to variations in growth-light conditions. Photosynth Res 52(2):117–125
  • Ernstsen J, Woodrow IE, Mott KA (1999) Effects of growth-light quantity, growth-light quality and CO₂ concentration on Rubisco deactivation during low PFD or darkness. Photosynth Res 61:65–75
  • Fukayama H, Ueguchi C, Nishikawa K, Katoh N, Ishikawa C, Masumoto C, Hatanaka T, Misoo S (2012) Overexpression of Rubisco activase decreases the photosynthetic CO₂ assimilation rate by reducing Rubisco content in rice leaves. Plant Cell Physiol 53:976–986
  • Gao LH, Qu M, Ren HZ, Sui XL, Chen QY, Zhang ZX (2010) Structure, function, application, and ecological benefit of a single-slope, energy-efficient solar greenhouse in China. HortTech 20:626–631
  • Genty B, Briantais JM, Baker NR (1989) The relationship between the quantum yield of photosynthetic electron-transport and quenching of chlorophyll fluorescence. Biochim Biophys Acta 990:87–92
  • Han QM, Yamaguchi E, Odaka N, Kakubari Y (1999) Photosynthetic induction responses to variable light under field conditions in three species grown in the gap and understory of a Fagus crenata forest. Tree Physiol 19:625–634
  • Hudson GS, Evans JR, Voncaemmerer S, Arvidsson YBC, Andrews TJ (1992) Reduction of ribulose-1,5-bisphosphate carboxylase oxygenase content by antisense RNA reduces photosynthesis in transgenic tobacco plants. Plant Physiol 98(1):294–302
  • Izumi M, Tsunoda H, Suzuki Y, Makino A, Ishida H (2012) RBCS1A and RBCS3B, two major members within the Arabidopsis RBCS multigene family, function to yield sufficient Rubisco content for leaf photosynthetic capacity. J Exp Bot 63:2159–2170
  • Kosvancova M, Urban O, Sprtova M, Hrstka M, Kalina J, Tomaskova I, Spunda V, Marek MV (2009) Photosynthetic induction in broadleaved Fagus sylvatica and coniferous Picea abies cultivated under ambient and elevated CO₂ concentrations. Plant Sci 177:123–130
  • Krall JP, Sheveleva EV, Pearcy RW (1995) Regulation of photosynthetic induction state in high-light-grown and low-light-grown soybean and Alocasia-Macrorrhiza (L.) Don. G. Plant Physiol 109:307–317
  • Parry MAJ, Keys AJ, Madgwick PJ, Carmo-Silva AE, Andralojc PJ (2008) Rubisco regulation: a role for inhibitors. J Exp Bot 59:1569–1580
  • Patel M, Berry JO (2008) Rubisco gene expression in C4 plants. J Exp Bot 59:1625–1634
  • Pearcy RW, Seemann JR (1990) Photosynthetic induction state of leaves in a soybean canopy in relation to light regulation of ribulose-1-5-bisphosphate carboxylase and stomatal conductance. Plant Physiol 94:628–633
  • Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29:2002–2007
  • Portis AR, Li CS, Wang DF, Salvucci ME (2008) Regulation of Rubisco activase and its interaction with Rubisco. J Exp Bot 59:1597–1604
  • Prins A, van Heerden PD, Olmos E, Kunert KJ, Foyer CH (2008) Cysteine proteinases regulate chloroplast protein content and composition in tobacco leaves: a model for dynamic interactions with ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) vesicular bodies. J Exp Bot 59:1935–1950
  • Roden JS, Pearcy RW (1993) Photosynthetic gas-exchange response of poplars to steady-state and dynamic light environments. Oecologia 93(2):208–214
  • Rodermel S (1999) Subunit control of Rubisco biosynthesis: a relic of an endosymbiotic past? Photosynth Res 59:105–123
  • Rodermel SR, Abbott MS, Bogorad L (1988) Nuclear–organelle interactions: nuclear antisense gene inhibits ribulose bisphosphate carboxylase enzyme levels in transformed tobacco plants. Cell 55:673–681
  • Sassenrathcole GF, Pearcy RW (1992) The role of ribulose-1,5-bisphosphate regeneration in the induction-requirement of photosynthetic CO₂ exchange under transient light conditions. Plant Physiol 99:227–234
  • Sassenrathcole GF, Pearcy RW (1994) Regulation of photosynthetic induction state by the magnitude and duration of low-light exposure. Plant Physiol 105:1115–1123
  • Sui XL, Sun JL, Wang SH, Li W, Hu LP, Meng FZ, Fan YY, Zhang ZX (2011) Photosynthetic induction in leaves of two cucumber genotypes differing in sensitivity to low-light stress. Afr J Biotechnol 10:2238–2247
  • Suzuki Y, Makino A (2013) Translational downregulation of RBCL is operative in the coordinated expression of Rubisco genes in senescent leaves in rice. J Exp Bot 4:1145–1152
  • Suzuki Y, Makino A, Mae T (2001) Changes in the turnover of Rubisco and levels of mRNAs of rbcL and rbcS in rice leaves from emergence to senescence. Plant, Cell Environ 24:1353–1360
  • Tinocoojanguren C, Pearcy RW (1992) Dynamic stomatal behavior and its role in carbon gain during lightflecks of a gap phase and an understory Piper species acclimated to high and low light. Oecologia 92:222–228
  • Urban O, Kosvancova M, Marek MV, Lichtenthaler HK (2007) Induction of photosynthesis and importance of limitations during the induction phase in sun and shade leaves of five ecologically contrasting tree species from the temperate zone. Tree Physiol 27:1207–1215
  • Valladares F, Allen MT, Pearcy RW (1997) Photosynthetic responses to dynamic light under field conditions in six tropical rainforest shrubs occurring along a light gradient. Oecologia 111:505–514
  • Woodrow IE, Mott KA (1989) Rate limitation of non-steady-state photosynthesis by ribulose-1,5-bisphosphate carboxylase in spinach. Aust J Plant Physiol 16:487–500
  • Wostrikoff K, Stern DB (2007) Rubisco large-subunit translation is autoregulated in response to its assembly state in tobacco chloroplasts. Proc Natl Acad Sci USA 104:6466–6471
  • Yamori W, Von Caemmerer S (2009) Effect of Rubisco activase deficiency on the temperature response of CO₂ assimilation rate and Rubisco activation state: insights from transgenic tobacco with reduced amounts of Rubisco activase. Plant Physiol 151:2073–2082
  • Yamori W, Masumoto C, Fukayama H, Makino A (2012) Rubisco activase is a key regulator of non-steady-state photosynthesis at any leaf temperature and, to a lesser extent, of steady state photosynthesis at high temperature. Plant J 71:871–880
  • Zhang N, Portis AR (1999) Mechanism of light regulation of Rubisco: a specific role for the larger Rubisco activase isoform involving reductive activation by thioredoxin-f. Proc Natl Acad Sci USA 96:9438–9443
  • Zhang N, Schurmann P, Portis AR (2001) Characterization of the regulatory function of the 46-kDa isoform of Rubisco activase from Arabidopsis. Photosynth Res 68:29–37
  • Zhang N, Kallis RP, Ewy RG, Portis AR (2002) Light modulation of Rubisco in Arabidopsis requires a capacity for redox regulation of the larger Rubisco activase isoform. Proc Natl Acad Sci USA 99:3330–3334
  • Zhou YH, Yu JQ, Huang LF, Nogues S (2004) The relationship between CO₂ assimilation, photosynthetic electron transport and water–water cycle in chill-exposed cucumber leaves under low light and subsequent recovery. Plant Cell Environ 27:1503–1514
  • Zhang LD, Zhang LX, Sun JL, Zhang ZX, Ren HZ, Sui XL (2013) Rubisco gene expression and photosynthetic characteristics of cucumber seedlings in response to water deficit. Sci. Hortic 161:81–87
  • Zhou YH, Yu JQ, Mao WH, Huang LF, Song XS, Nogues S (2006) Genotypic variation of Rubisco expression, photosynthetic electron flow and antioxidant metabolism in the chloroplasts of chill-exposed cucumber plants. Plant Cell Physiol 47:192–199
Typ dokumentu
Bibliografia
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