In vitro effects of prolonged exposure to quercetin and epigallocatechin gallate of the peripheral blood mononuclear cell membrane

• Autorzy: Margina D. , Ilie M. , Manda G. , Neagoe I. , Danciulescu-Miulescu R. , Purdel C. N. , Gradinaru D.
• Języki publikacji: EN

Abstrakty

EN

The study aimed to assess biophysical changes that take place in the peripheral blood mononuclear cell (PBMC) membranes when exposed in vitro to 10 μM quercetin or epigallocatechin gallate (EGCG) for 24 and 48 h. PBMCs isolated from hypercholesterolemia patients were compared to those from normocholesterolemia subjects. The membrane fluidity and transmembrane potential were evaluated and the results were correlated with biochemical parameters relevant to oxidative stress, assessed in the patients’ plasma. The baseline value of PBMC membrane anisotropy for the hypercholesterolemia patients was lower than that of the control group. These results correlated with the plasma levels of advanced glycation end products, which were significantly higher in the hypercholesterolemia group, and the total plasma antioxidant status, which was significantly higher in normocholesterolemia subjects. In the case of normocholesterolemia cells in vitro, polyphenols induced a decrease in membrane anisotropy (7.25–11.88% at 24 h, 1.82–2.26% at 48 h) and a hyperpolarizing effect (8.30–8.90% at 24 h and 4.58–13.00% at 48 h). The same effect was induced in hypercholesterolemia cells, but only after 48 h exposure to the polyphenols: the decrease in membrane anisotropy was 5.70% for quercetin and 2.33% for EGCG. After 48 h of in vitro incubation with the polyphenols, PBMCs isolated from hypercholesterolemia patients exhibited the effects that had been registered in cells from normocholesterolemia subjects after 24 h exposure. These results outlined the beneficial action of the studied polyphenols, quercetin and EGCG, as dietary supplements in normocholesterolemia and hypercholesterolemia patients.

Słowa kluczowe

EN

in vitro effect; exposure; quercetin; epigallocatechin gallate; peripheral blood; mononuclear cell; cell membrane

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2014
• Tom: 19
• Numer: 4
• Opis fizyczny: p.542-560,ref.

Twórcy

autor

Margina D.

• Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6 Traian Vuia St., 020956, Bucharest, Romania

autor

Ilie M.

• Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6 Traian Vuia St., 020956, Bucharest, Romania

autor

Manda G.

• Victor Babes National Institute of Pathology, Bucharest, 99-101 Splaiul Independentei, 050096, Bucharest, Romania

autor

Neagoe I.

• Victor Babes National Institute of Pathology, Bucharest, 99-101 Splaiul Independentei, 050096, Bucharest, Romania

autor

Danciulescu-Miulescu R.

• N.Paulescu National Institute for Diabetes, Nutrition and Metabolic Diseases, 5-7 Ion Movila St., 020475, Bucharest, Romania

autor

Purdel C. N.

• Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6 Traian Vuia St., 020956, Bucharest, Romania

autor

Gradinaru D.

• Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6 Traian Vuia St., 020956, Bucharest, Romania

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Identyfikatory

DOI

10.2478/s11658-014-0211-7