A rapid ion exchange procedure that facilitates spectrophotometric assays of phosphorylated metabolites in potato extracts

• Autorzy: Dorion S. , Rivoal J.
• Języki publikacji: EN

Abstrakty

EN

A variety of metabolites are routinely assayed after perchloric acid extraction of plant tissues. A common technique uses coupled enzyme assays that produce or consume pyridine nucleotides together with spectrophotometric detection at 340 nm. Because of the presence of pigments in plant tissues, the high absorbance of such extracts usually limits the amount of extract that can be assayed spectrophotometrically. Here, we show that after batch adsorption with AG50WX8 (H⁺ form), the absorbance of potato root perchloric acid extracts at 340 nm is significantly reduced. This clean up procedure does not interfere with the assay or the recovery of anionic metabolites such as hexose phosphates. It therefore facilitates spectrophotometric assays of metabolites in plant extracts with high absorbance.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2009
• Tom: 31
• Numer: 4
• Opis fizyczny: p.855-859,fig.,ref.

Twórcy

autor

Dorion S.

• IRBV, Universite de Montreal, 4101 rue Sherbrooke est, Montreal, QC H1X 2B2, Canada

autor

Rivoal J.

• IRBV, Universite de Montreal, 4101 rue Sherbrooke est, Montreal, QC H1X 2B2, Canada

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