Molekularne metody wykrywania Listeria monocytogenes w żywności

• Autorzy: Lachtara B. , Wieczorek K. , Osek J.

Warianty tytułu

EN

Molecular methods for the detection of Listeria monocytogenes in food

• Języki publikacji: PL

Abstrakty

EN

Listeria monocytogenes is an important foodborne pathogen that causes a disease known as listeriosis, which is especially dangerous for pregnant women. Infection with L. monocytogenes may also result in stillbirths, abortions and premature deliveries, as well as meningitis, septicaemia, encephalitis, and meningoencephalitis. Conventional detection methods of these bacteria are time-consuming; therefore, rapid alternative methods, including those based on molecular tests are needed. PCR is sensitive and specific; however, it requires the use of an agarose gel, which increases the time of analysis. A technique that allows the elimination of this step is real-time PCR, which enables the quantitative determination of L. monocytogenes in foods. A modification of PCR is multiplex PCR that allows detection of several genes at the same time and distinguishes between different species of microorganisms. Techniques such as RT-PCR or NASBA, where the target molecule is RNA, are used to detect viable cells and also allow quantitative analyses to be performed. Another rapid and specific method is LAMP, which can be performed in one hour in a water bath or heating block, without the use of a thermocycler. Biosensors and microarrays are examples of new technologies that due to the possibility to use anywhere and immediate interpretation of the results can be routinely used in the future for identification of L. monocytogenes in food.

Słowa kluczowe

PL

zywnosc; zanieczyszczenia bakteryjne; Listeria monocytogenes; wykrywanie; identyfikacja; metody molekularne; lancuchowa reakcja polimerazy; metoda multiplex PCR; metoda real time PCR; metoda RT-PCR; metoda NASBA; metoda LAMP; hybrydyzacja DNA; mikromacierze DNA; biosensory

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2016
• Tom: 72
• Numer: 01
• Opis fizyczny: s.12-17,tab.,bibliogr.

Twórcy

autor

Lachtara B.

• Zakład Higieny Żywności Pochodzenia Zwierzęcego, Państwowy Instytut Weterynaryjny - Państwowy Instytut Badawczy w Puławach, Al. Partyzantów 57, 24-100 Puławy

autor

Wieczorek K.

• Zakład Higieny Żywności Pochodzenia Zwierzęcego, Państwowy Instytut Weterynaryjny - Państwowy Instytut Badawczy w Puławach, Al. Partyzantów 57, 24-100 Puławy

autor

Osek J.

• Zakład Higieny Żywności Pochodzenia Zwierzęcego, Państwowy Instytut Weterynaryjny - Państwowy Instytut Badawczy w Puławach, Al. Partyzantów 57, 24-100 Puławy

Bibliografia

• Alarcón B., García-Cañas V., Cifuentes A., González R., Aznar R.: Simultaneous and sensitive detection of three foodborne pathogens by multiplex PCR, capillary gel electrophoresis, and laser-induced fluorescence. J. Agric. Food Chem. 2004, 52, 7180-7186.
• Aznar R., Alarcón B.: PCR detection of Listeria monocytogenes: a study of multiple factors affecting sensitivity. J. Appl. Microbiol. 2003, 95, 958-966.
• Barbau-Piednoir E., Botteldoorn N., Yde M., Mahillon J., Roosens N. H.: Development and validation of qualitative SYBR®Green Real-Time PCR for detection and discrimination of Listeria spp. and Listeria monocytogenes. Appl. Microbiol. Biotechnol. 2013, 97, 4021-4037.
• Bhagwat A. A.: Simultaneous detection of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella strains by real-time PCR. Int. J. Food Microbiol. 2003, 84, 217-224.
• Blais B. W., Turner G., Sooknanan R., Malek L. T.: A nucleic acid sequence-based amplification system for detection of Listeria monocytogenes hlyA sequences. Appl. Environ. Microbiol. 1997, 63, 310-313.
• Bubert A., Hein I., Rauch M., Lehner A., Yoon B., Goebel W., Wagner M.: Detection and differentiation of Listeria spp. by a single reaction based multiplex PCR. Appl. Environ. Microbiol. 1999, 65, 4688-4692.
• Call D. R., Borucki M. K., Loge F. J.: Detection of bacterial pathogens in environmental samples using DNA microarrays. J. Microbiol. Methods 2003, 53, 235-243.
• Churchill R. L. T., Lee H., Hall J. C.: Detection of Listeria monocytogenes and the toxin listeriolysin O in food. J. Microbiol. Methods 2006, 64, 141-170.
• Cocolin L., Rantsiou K., Iacumin L., Cantoni C., Comi G.: Direct identification in food samples of Listeria spp. and Listeria monocytogenes by molecular methods. Appl. Environ. Microbiol. 2002, 68, 6273-6282.
• Collins R. A., Ko L.-S., So K.-L., Ellis T., Lau L.-T., Cheung Hoi Yu A.: Detection of highly pathogenic and low pathogenic avian influenza subtype H5 (Eurasian lineage) using NASBA. J. Virol. Methods 2002, 103, 213-225.
• Cook N.: The use of NASBA for the detection of microbial pathogens in food and environmental samples. J. Microbiol. Methods 2003, 53, 165-174.
• EFSA Panel on EFSA Biological Hazards (BIOHAZ): Scientific Opinion on the evaluation of molecular typing methods for major food-borne microbiological hazards and their use for attribution modelling, outbreak investigation and scanning surveillance: Part 1 (evaluation of methods and applications). EFSA J. 2013, 11, 3502.
• European Food Safety Authority: The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2012. EFSA J. 2014, 12, 3547.
• Fakruddin M. D., Mazumdar R. M., Chowdhury A., Bin Mannan K. S.: Nucleic and acid sequence based amplification (NASBA) – prospects and applications. Int. J. Pharm. Life Sci. 2012, 2, 106-121.
• Gasanov U., Hughes D., Hansbro P. M.: Methods for the isolation and identification of Listeria spp. and Listeria monocytogenes: a review. FEMS Microbiol. Rev. 2005, 29, 851-875.
• Hein I., Klein D., Lehner A., Bubert A., Brandl E., Wagner M.: Detection and quantification of the iap gene of Listeria monocytogenes and Listeria innocua by a new real-time quantitative PCR assay. Res. Microbiol. 2001, 152, 37-46.
• Ivnitski D., Abdel-Hamid I., Atanasov P., Wilkins E.: Biosensors for detection of pathogenic bacteria. Biosens. Bioelectron. 1999, 14, 599-624.
• Jadhav S., Bhave M., Palombo E. A.: Methods used for the detection and subtyping of Listeria monocytogenes. J. Microbiol. Methods 2012, 88, 327-341.
• Janzten M. M., Navas J., Corujo A., Moreno R., López V., Martínez-Suárez J. V.: Review. Specific detection of Listeria monocytogenes in foods using commercial methods: from chromogenic media to real-time PCR. Span. J. Agric. Res. 2006, 4, 235-247.
• Jeyaletchumi P., Tunung R., Margaret S. P., Son R., Farinazleen M. G., Cheah Y. K.: Detection of Listeria monocytogenes in foods. Int. Food Res. J. 2010, 17, 1-11.
• Jofré A., Martin B., Garriga M., Hugas M., Pla M., Rodríguez-Lázaro D., Aymerich T.: Simultaneous detection of Listeria monocytogenes and Salmonella by multiplex PCR in cooked ham. Food Microbiol. 2005, 22, 109-115.
• Jung Y. S., Frank J. F., Brackett R. E., Chen J.: Polymerase chain reaction detection of Listeria monocytogenes in frankfurters using oligonucleotide primers targetting the genes encoding internalin AB. J. Food Prot. 2003, 66, 237-241.
• Keer J. T., Birch L.: Molecular methods for the assessment of bacterial viability. J. Microbiol. Methods 2003, 53, 175-183.
• Klein P. G., Juneja V. K.: Sensitive detection of viable Listeria monocytogenes by reverse transcription-PCR. Appl. Environ. Microbiol. 1997, 63, 4441-4448.
• Lei I. F., Roffey P., Blanchard C., Gu K.: Development of a multiplex PCR method for the detection of six common foodborne pathogens. J. Food Drug Anal. 2008, 16, 37-43.
• Liu D.: Identification, subtyping and virulence determination of Listeria monocytogenes, an important foodborne pathogen. J. Med. Microbiol. 2006, 55, 645-659.
• Liu D., Ainsworth A. J., Austin F. W., Lawrence M. L.: Identification of Listeria innocua by PCR targeting a putative transcriptional regulator gene. FEMS Microbiol. Lett. 2003, 223, 205-210.
• Liu D., Ainswortha A. J., Austinb F. W., Lawrence M. L.: Use of PCR primers derived from a putative transcriptional regulator gene for species-specific determination of Listeria monocytogenes. Int. J. Food Microbiol. 2004, 91, 297-304.
• Momtaz H., Yadollahi S.: Molecular characterization of Listeria monocytogenes isolated from fresh seafood samples in Iran. Diagn. Pathol. 2013, 8, 149.
• Nogva H. K., Rudi K., Naterstad K., Holck A., Lillehaug D.: Application of 5’-nuclease PCR for quantitative detection of Listeria monocytogenes in pure cultures, water, skim milk, and unpasteurized whole milk. Appl. Environ. Microbiol. 2000, 66, 4266-4271.
• Ohk S. H., Koo O. K., Sen T., Yamamoto C. M., Bhunia A. K.: Antibody-aptamer functionalized fibre-optic biosensor for specific detection of Listeria monocytogenes from food. J. Appl. Microbiol. 2010, 109, 808-817.
• Pochop J., Kačániová M., Hleba L., Petrová J., Lopašovský L., Pavelková A., Bobková A.: Real-time PCR Detection of Listeria monocytogenes in food samples of animal origin. J. Microb. Biotech. Food Sci. 2013, Special issue 1, 1550-1558.
• Raszka A., Ziembińska A., Wiechetek A.: Metody i techniki biologii molekularnej w biologii środowiskowej. Czasopismo Techniczne 2-Ś 2009, 2, 101-114.
• Rawool D. B., Malik S. V. S., Shakuntala I., Sahare A. M., Barbuddhe S. B.: Detection of multiple virulence-associated genes in Listeria monocytogenes isolated from bovine mastitis cases. Int. J. Food Microbiol. 2007, 113, 201-207.
• Rodríguez-Lázaro D., Jofré A., Aymerich T., Hugas M., Pla M.: Rapid quantitative detection of Listeria monocytogenes in meat products by real-time PCR. Appl. Environ. Microbiol. 2004, 70, 6299-6301.
• Romanek J.: Real-time PCR – nowoczesna technika analizy ekspresji genów na poziomie transkryptu. Wiad. Zoot. 2011, 1, 125-129.
• Rossmanith P., Krassnig M., Wagner M., Hein I.: Detection of Listeria monocytogenes in food using a combined enrichment/real-time PCR method targeting the prfA gene. Res. Microbiol. 2006, 157, 763-771.
• Rozporządzenie Komisji (WE) NR 2073/2005 z dnia 15 listopada 2005 r. w sprawie kryteriów mikrobiologicznych dotyczących środków spożywczych. Dz. Urz. UE L 338 z dnia 22 grudnia 2005 r., 1-26.
• Singh J., Batish V. K., Grover S.: Molecular beacon based real-time PCR assay for simultaneous detection of Listeria monocytogenes and Salmonella spp. in dairy products. Dairy Sci. Technol. 2011, 91, 373-382.
• Strom M. S.: Phenotypic and genetic characterization of a non-hemolytic variant of Listeria monocytogenes from cold-smoked salmon. Food Microbiol. 1998, 15, 329-337.
• Studzińska A., Tyburski J., Daca P., Tretyn A.: PCR w czasie rzeczywistym. Istota metody i strategie monitorowania przebiegu reakcji. Biotechnologia 2008, 80, 71-85.
• Suo B., He Y., Paoli G., Gehring A., Tu S.-I., Shi X.: Development of an oligonucleotide-based microarray to detect multiple foodborne pathogens. Mol. Cell. Probes 2010, 24, 77-86.
• Tang M. J., Zhou S., Zhang X. Y., Pu J. H., Ge Q. L., Tang X. J., Gao Y. S.: Rapid and sensitive detection of Listeria monocytogenes by loop-mediated-isothermal amplification. Curr. Microbiol. 2011, 63, 511-516.
• Uyttendaele M., Schukkink R., van Gemen B., Debevere J.: Development of NASBA®, a nucleic acid amplification system, for identification of Listeria monocytogenes and comparison to ELISA and a modified FDA method. Int. J. Food Microbiol. 1995, 27, 77-89.
• Vasconcelos R. M. de, de Almeida A. E. C. C., Hofer E., da Silva N. M. M., Marin V. A.: Multiplex-PCR serotyping of Listeria monocytogenes isolated from human clinical specimens. Mem. Inst. Oswaldo Cruz. 2008, 103, 836-838.
• Vázquez-Boland J. A., Kuhn M., Berche P., Chakraborty T., Domínguez-Bernal G., Goebel W., González-Zorn B., Wehland J., Kreft J.: Listeria pathogenesis and molecular virulence determinants. Clin. Microbiol. Rev. 2008, 14, 584-640.
• Wang C., Hong C.: A rapid PCR-based hybridization assay for the detection of Listeria monocytogenes in channel catfish. Food Microbiol. 1999, 16, 291-297.
• Wang L., Li Y., Chu J., Xu Z., Zhong Q.: Development and application of a simple loop-mediated isothermal amplification method on rapid detection of Listeria monocytogenes strains. Mol. Biol. Rep. 2012, 39, 445-449.
• Zarei M., Basiri N., Jamnejad A., Eskandari M. H.: Prevalence of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella spp. in beef, buffalo and lamb using multiplex PCR. Jundishapur J. Microbiol. 2013, 6, 7244.
• Zhang D., Zhang H., Yang L., Guo J., Li X., Feng Y.: Simultaneous detection of Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica and Escherichia coli O157:H7 in food samples using multiplex PCR method. J. Food Safety 2009, 29, 348-363.
• Zhou X., Jiao X.: Polymerase chain reaction detection of Listeria monocytogenes using oligonucleotide primers targeting actA gene. Food Control 2005, 16, 125-130.