Genetic transformation of the sweet orange cultivars from explants of adlut plants

• Autorzy: Favero P. , de Assis Alves Mourao Filho F. , Stipp L. C. , Mendes B. M.
• Języki publikacji: EN

Abstrakty

EN

The difficulty in adult tissue genetic transformation in woody species is still an obstacle to be overcome, including in most sweet orange cultivars of the Brazilian citrus industry. This work reports that, after in vitro culture adjustments, transgenic adventitious buds of ‘Hamlin’, ‘Pêra’, and ‘Valencia’ sweet oranges (Citrus sinensis L. Osbeck) were recovered using adult material as explant source, in genetic transformation experiments via Agrobacterium tumefaciens. The transgenic buds were identified by the GUS histochemical analysis and confirmed by PCR analysis, which indicated the presence of an amplified fragment of 817 bp corresponding to the uidA gene sequence. The efficiencies of genetic transformation for ‘Hamlin’, ‘Pêra’, and ‘Valencia’ sweet orange cultivars were 2.5, 1.4, and 3.7%, respectively. Media supplemented with auxins and cytokinins during co-culture, and media with high concentrations of cytokinins (3 mg L⁻¹) during transgenic selection led to the transformation and, consequently, the regeneration of adequate number of adventitious buds for the three cultivars. The use of sonication during the explant disinfection was not effective to reduce endophytic contamination and reduced transformation efficiency.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 2
• Opis fizyczny: p.471-477,fig.,ref.

Twórcy

autor

Favero P.

• Escola Superior de Agricultura "Luiz de Queiroz", Universidade de Sao Paulo, Piracicaba, 13418-900 Sao Paulo, Brazil

autor

de Assis Alves Mourao Filho F.

• Escola Superior de Agricultura "Luiz de Queiroz", Universidade de Sao Paulo, Piracicaba, 13418-900 Sao Paulo, Brazil

autor

Stipp L. C.

• Escola Superior de Agricultura "Luiz de Queiroz", Universidade de Sao Paulo, Piracicaba, 13418-900 Sao Paulo, Brazil

autor

Mendes B. M.

• Centro de Energia Nuclear na Agricultura, Universidade de Sao Paulo, Piracicaba, 13416-000 Sao Paulo, Brazil

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